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21.
Yan-Hong He Guo-Gui Ning Ya-Lin Sun Yan Hu Xing-Yu Zhao Man-Zhu Bao 《Molecular breeding : new strategies in plant improvement》2010,26(1):19-29
A male sterile line was isolated in marigold (Tagetes erecta L.) and cytological analysis determined this to be a novel genic male sterility trait (Tems). Through the use of amplified fragment length polymorphisms (AFLPs) and bulked segregant analysis (BSA), tightly linked
markers of Tems were identified with a view towards a map-based cloning strategy. It was found that spontaneous homeotic conversion of floral
organs was the underlying cause of the male sterility in this marigold line. Thus, petals of male sterile plants resembled
sepal-like structures and the stamens were partially converted to styles, although without the full characteristics or function
of the true style organs. We have constructed a fine marker-based map for the Tems gene. This is intended to provide a tool for marker assisted selection (MAS) strategies in hybrid breeding and map-based
cloning strategies for the male sterility locus. We discuss the significance of this spontaneously derived genic male sterility
trait relating to the homeotic conversion of floral organs in marigold. 相似文献
22.
23.
Scavenging effect of extracts of green tea and natural antioxidants on active oxygen radicals 总被引:14,自引:0,他引:14
With the use of the spin trapping methods, the scavenging effects of the extracts of green tea and other natural foods are studied. In stimulated polymorphonuclear leukocytes (PMN) system, water extract fraction 6 (F6) from green tea and green tea polyphenols (GTP) have the strongest scavenging effect on the active oxygen radicals, much stronger than vitamin C (Vc) and vitamin E (VE). Rosemary antioxidants (RA) and Curcumin (Cur) have weaker scavenging effects than Vc, but stronger than VE. In Fenton Reaction, Cur has the strongest scavenging effect (69%) on hydroxyl radicals. In irradiation, riboflavin system F6(74%) and GTP(72%) have very strong scavenging effects that are weaker than Vc, but much stronger than VE (23%). With the use of spin probe oxymetry, the oxygen consumption in respiratory burst of stimulated PMN were measured when the antioxidants existed in these systems. The results demonstrated that these antioxidants did not affect the respiratory burst of human polymorphonuclear leukocytes stimulated with PMA. 相似文献
24.
延胡索分类的化学证据 总被引:2,自引:0,他引:2
东阳产延胡索与大连产齿瓣延胡索经成分分离和TLC、HPLC对比,发现延胡索以啊扑啡类生物碱如glaucine为主,而齿瓣延胡索则含corynoline类生物碱。根据生物碱的类型及含量比较,二者有明显差异,结合延胡索的植物形态和植化分类特征判断,将延胡索作为与齿瓣延胡索近缘的独立种处理较为合理,即为Corydalis yanhusuo W. T. Wang ex Z.Y. Su et C. Y. Wu 相似文献
25.
大壁虎的染色体及减数分裂联会复合体的研究 总被引:7,自引:1,他引:6
大壁虎(Gekko gecko)的染色体数目为2n=38,核型由2对中着丝粒(Nos.1.4.)、3对亚中着丝粒(Nos.2.3.5)及14对端着丝粒和亚端着丝粒(Nos.6—19)染色体组成。一对核仁组织者(NOR_s),位于第7对端着丝粒染色体的末端。同时,本文还对大壁虎的减数分裂以及联会复合体(S.C)的结构和组型,进行了详细的观察和分析。 相似文献
26.
Assay of L-3-hydroxyacyl-coenzyme A dehydrogenase with substrates of different chain lengths 总被引:1,自引:0,他引:1
A method for assaying L-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) which permits rate measurements with L-3-hydroxyacyl-CoA substrates of various chain lengths at physiological pH is described. The method is based on a coupled assay system in which 3-ketoacyl-CoA compounds formed by the dehydrogenase are cleaved by 3-ketoacyl-CoA thiolase (EC 2.3.1.16) in the presence of CoASH. The advantages of this assay method are its irreversibility and elimination of product inhibition. The assay procedure was used to determine the kinetic parameters (Km, Vmax) of pig heart L-3-hydroxyacyl-CoA dehydrogenase with several substrates of various chain lengths. The data obtained show the enzyme to be most active with medium-chain substrates whereas Km values for medium-chain and long-chain substrates are almost equal but much lower than those previously reported. 相似文献
27.
Effects of acetylene on hydrogenases from the sulfate reducing and methanogenic bacteria 总被引:1,自引:0,他引:1
S H He S B Woo D V DerVartanian J Le Gall H D Peck 《Biochemical and biophysical research communications》1989,161(1):127-133
The effect of acetylene on the activity of the three types of hydrogenase from the anaerobic sulfate reducing bacteria has been investigated. The (Fe) hydrogenase is resistant to inhibition by acetylene while the nickel-containing hydrogenases are inhibited by acetylene with the (NiFe) hydrogenase being 10-50 fold more sensitive than the (NiFeSe) hydrogenase. In addition the Ni(III) EPR signal (g approximately 2.3) of the "as isolated" (NiFe) hydrogenase was significantly decreased in intensity upon exposure to acetylene. 相似文献
28.
29.
Endurance and swimming speed were measured in mackerel, herring and saithe when they were induced by the optomotor response to swim at prolonged speeds along a 28-m circular track through still water in a 10-m diameter gantry tank. The maximum sustained swimming speed ( U ms was measured as body lengths per second ( b.l.s −1 ) for each species and for saithe of different size groups. Herring with U ms of 4.06 b.l.s −1 (25.3 cm, 13.5°C) were the fastest, mackerel U ms was 3.5 b.l.s 1 (33 cm, 11.7°C) and saithe (14.4°C) showed a size effect where U ms at 25 cm was 3.5 b.l.s 1 and at 50 cm 2.2 b.l.s 1 . When swimming at speeds higher that U ms , all three species showed reduced endurance as speed increased. How the curved track reduces the swimming speed is discussed. 相似文献
30.
对根霉(Rhizopus)属的十个种或变种共二十四株菌的菌体可溶性蛋白和酯酶同工酶进行了电泳的研究得到对这个属分类上更多的依据.在严格控制培养,提取和电泳条件的情况下,同一株菌不同批次所得菌体蛋白电泳图谱有较好的重复性.在相同的条件下,每个种的根霉有各自特征性蛋白图谱,种内不同菌株的蛋白图谱和酯酶酶谱基本相同.特别是形态特征明显、分类地位明确的种,种内各株的图谱也较一致,如R. stolonifer;与R.circinans.在确定新变种R. delemar var. latoapicalis时,电泳图谱与R.delemar var.delema:有明显不同,起到了佐证作用.因此认为,蛋白图谱与酯酶酶谱相辅相成,在根霉种的分类中是一有效的辅助手段. 相似文献