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91.
Bladder cancer-associated protein gene (BLCAP) is a novel candidate tumor suppressor gene identified from the human bladder
carcinoma. Our previous studies have shown that BLCAP overexpression could inhibit cell growth by inducing apoptosis in HeLa
cells [Zuo Z, Zhao M, Liu J, Gao G, Wu X: Tumor Biol 27: 221–226, 2006]. Such evidence suggests the alterations in BLCAP may
play an important role in tumorigenesis. To further study the biological function of the BLCAP gene, we constructed a recombinant
retroviral vector encoding BLCAP cDNA. Overexpressed BLCAP, via stable infection of exogenous BLCAP, resulted in growth inhibition
of the human tongue cancer cell line Tca8113 in vitro, accompanied by S phase cell cycle arrest and apoptosis. The growth inhibition was correlated with up-regulation of p21WAF1/CIP1 expression and down-regulation of Bcl-XL and Bcl-2 expressions. However, p53 expression and NF-κB activity remained unchanged
post infection. Furthermore, no changes in p53 phosphorylation at Ser46 and nuclear localization, which are critical to p53
function, were observed in BLCAP-overexpressed cells. Taken together, BLCAP may play a role not only in regulating cell proliferation
but also in coordinating apoptosis and cell cycle via a novel way independent of p53 and NF-κB.
Jun Yao and Li Duan contributed equally to this work. 相似文献
92.
93.
环化腺苷酸对细菌生长的影响 总被引:1,自引:0,他引:1
用大肠杆菌(Escherichia coli AS 1.797)、北京棒状杆菌(Corynebacterium pekinense AS1.299)和巨大芽孢杆菌(Bacills megatertum AS 1.217)研究了细胞内环化腺苷酸(cAMP)浓度和外源cAMP对细胞生长的影响。结果表明,大肠杆菌在不同碳源中生长时,细胞的生长量随细胞内cAMF’浓度升高而降低。在以葡萄糖作碳源时,细胞内cAMP浓度低,外源cAMP。对生长有抑制作用,而cAMP的类似物5'-AMP则无抑制作用。在以乳糖、麦芽糖和甘油分别作碳源时,细胞内cAMP浓度高,外源cAMP对生长无影响。北京捧状杆菌以葡萄糖作碳源时,细胞生长也受外源cAMP的抑制,但cAMP的抑制作用不是专一的,它的作用可用类似物5’-AMP来代替。自身不合cAMP的巨大芽孢杆菌在不同碳原(包括葡萄糖)中生长时,生长不受外源cAMP抑制,也不受5’-Amt’的影响。因此认为,cAMP不是细菌生长的必需物,而是生长调节物,但这种调节物对巨大芽孢杆菌无效。 相似文献
94.
不同小麦品种对播娘蒿的影响 总被引:2,自引:0,他引:2
播娘蒿是黄淮麦区主要田间杂草,在管理粗放的麦田,播娘蒿密度可达100株·m-2以上,严重影响了小麦的产量和品质。因此,对小麦-杂草复合体中杂草的生长发育规律进行研究,寻找降低草害的有效途径引起许多学者极大关注[1,2],而目前有关防除麦田杂草的研究多集中于化学防治方面[3]。然而,出于环境保护和经济成本上的考虑,化学除草受到了挑战。研究人员[4,5]发现,不同物种在竞争力上存在差异,禾谷类作物属于竞争力很强的作物[6],冬小麦及冬黑麦又是其中竞争力最强的物种。就小麦栽培种而言,存在着不同的品种类型,研究不同小麦品种对杂草的抑制作… 相似文献
95.
R-环是由一个RNA:DNA杂交体和一条单链状态的DNA分子共同组成的三链核酸结构。其中, RNA:DNA杂交体的形成起因于基因转录所合成的RNA分子不能与模板分开, 或RNA分子重新与一段双链DNA分子中的一条链杂交。在基因转录过程中, 当转录泡遇到富含G碱基的非模板链区或位于某些与人类疾病有关的三核苷酸卫星DNA时, 转录泡后方累积的负超螺旋可促进R环形成。同时, 新生RNA分子未被及时加工、成熟或未被快速转运到细胞质等因素也会催生R环。研究表明, 细胞拥有多种管理R环的方法, 可以有效地管理R环的形成和处理已经形成的R环, 以尽量避免R环对DNA复制、基因突变和同源重组产生不利影响。文章重点分析了R-环的形成机制及R环对DNA复制、基因突变和同源重组的影响, 并针对R-环诱导的DNA复制在某些三核苷酸重复扩增有关的神经肌肉退行性疾病发生过程中的作用进行了分析和讨论。 相似文献
96.
In previous studies, we have shown that the inactivation of the adenosine A2A receptor exacerbates chronic cerebral hypoperfusion-induced white matter lesions (WMLs) by enhancing neuroinflammatory responses. However, the molecular mechanism underlying the effect of the adenosine A2A receptor remains unknown. Recent studies have demonstrated that cystatin F, a potent endogenous cysteine protease inhibitor, is selectively expressed in immune cells in association with inflammatory demyelination in central nervous system diseases. To understand the expression of cystatin F and its potential role in the effect of A2A receptor on WMLs induced through chronic cerebral hypoperfusion, we investigated cystatin F expression in the WMLs of A2A receptor gene knockout mice, the littermate wild-type mice and wild-type mice treated daily with the A2A receptor agonist or both CGS21680 and A2A receptor antagonist CGS21680 after chronic cerebral hypoperfusion. The results of quantitative-PCR and western blot analysis revealed that cystatin F mRNA and protein expression were significantly up-regulated in the WMLs after chronic cerebral hypoperfusion. In addition, cystatin F expression in the corpus callosum was significantly increased in A2A receptor gene knockout mice and markedly decreased in mice treated with SCH58261 on both the mRNA and protein levels. Additionally, CGS21680 counteracted the attenuation of cystatin F expression produced by SCH58261 after chronic cerebral hypoperfusion. Moreover, double immunofluorescence staining revealed that cystatin F was co-localized with the activated microglia marker CD11b. In conclusion, the cystatin F expression in the activated microglia is closely associated with the effect of the A2A receptors, which may be related to the neuroinflammatory responses occurring during the pathological process. CGS21680相似文献
97.
98.
Reppel M Sasse P Piekorz R Tang M Roell W Duan Y Kletke A Hescheler J Nürnberg B Fleischmann BK 《The Journal of biological chemistry》2005,280(43):36019-36028
S100A1 is an EF-hand type Ca2+-binding protein with a muscle-specific expression pattern. The highest S100A1 protein levels are found in cardiomyocytes, and it is expressed already at day 8 in the heart during embryonic development. Since S100A1 is known to be involved in the regulation of Ca2+ homeostasis, we tested whether extracellular S100A1 plays a role in regulating the L-type Ca2+ current (I(Ca)) in ventricular cardiomyocytes. Murine embryonic (day 16.5 postcoitum) ventricular cardiomyocytes were incubated with S100A1 (0.001-10 microM) for different time periods (20 min to 48 h). I(Ca) density was found to be significantly increased as early as 20 min (from -10.8 +/- 1 pA/pF, n = 18, to -22.9 +/- 1.4 pA/pF; +112.5 +/- 13%, n = 9, p < 0.001) after the addition of S100A1 (1 microM). S100A1 also enhanced I(Ca) current density in neonatal rat cardiomyocytes. Fluorescence and capacitance measurements evidenced a fast translocation of rhodamine-coupled S100A1 from the extracellular space into cardiomyocytes. S100A1 treatment did not affect cAMP levels. However, protein kinase inhibitor, a blocker of cAMP-dependent protein kinase A (PKA), abolished the S100A1-induced enhancement of I(Ca). Accordingly, measurements of PKA activity yielded a significant increase in S100A1-treated cardiomyocytes. In vitro reconstitution assays further demonstrated that S100A1 enhanced PKA activity. We conclude that the Ca2+-binding protein S100A1 augments transsarcolemmal Ca2+ influx via an increase of PKA activity in ventricular cardiomyocytes and hence represents an important regulator of cardiac function. 相似文献
99.
目的探讨炎症细胞因子白介素-1β(interleukin-1βIL-1β)对高糖刺激的人肾小管上皮细胞转分化的影响。-方法体外培养人肾近曲小管上皮细胞株(HKCs),随机分为正常对照组(5.5 mmol/L normal glucose);高糖组(30 mmol/L high glucose);高糖+IL-1β(5ng/ml)组。分别于处理后24h、48h、72h收集细胞,采用免疫细胞化学染色和Western蛋白印迹法检测细胞角蛋白-18(cytokeratin-18 CK-18)、α-平滑肌肌动蛋白(α-smooth muscle actinα-SMA)水平。结果高糖能够诱导肾小管上皮细胞α-SMA蛋白的合成增加,而肾小管上皮细胞的标志物CK-18的表达逐渐减少;IL-1β与高糖同时刺激可使肾小管上皮细胞α-SMA蛋白表达进一步增多,而其自身标志物CK-18的表达则明显下降。结论炎症因子IL-1β能增强高糖对肾小管上皮细胞转分化的作用。 相似文献
100.
Xiaopeng Ji Bin Zhao Zhaoping Cheng Biao Si Zhiheng Wang Yanhua Duan Pei Nie Haiou Li Shifeng Yang Hui Jiao Ximing Wang 《PloS one》2014,9(4)