Sensory innervation of the gastro-intestinal junction: new electrophysiological, histological and histochemical data]

The sensory innervation of the small intestine was studied in the cat with electrophysiological, histological and histochemical techniques. Thanks to the histochemical technique (peroxydase method) the exact number and proportion of splanchnic and vagal fibres was determined : the latter being about 9 times more numerous than the former. On the other hand the exact position of the corresponding cells was defined in the nodose and spinal ganglia by means of the previous technique and the microelectrophysiological method (recording of single units into the ganglia with extracellular glass microelectrodes). The splanchnic neurones were found in the T9, T10 and T11 ganglia whereas the vagal ones were chiefly located in the lower half of the nodose ganglia. The histological studies using electronic microscope showed many non-medullated endings, which were often found beneath the epithelium and in the lamina propria of the villi close to the blood vessels. This result is certainly the proof that numerous receptors (mechanoreceptors, chemoreceptors and even thermoreceptors do exist in the small intestine.     

中国种子植物区系定量化研究——Ⅰ区域性种子植物区系研究实用程序工作原理(QX—系列程序)

本文根据吴征镒教授对中国种子植物属分市区类型研究结果,研制出定量化研究区域性种子植物区系的电子计算机程序,应用本程序可完成某区系的分布区类型统计分析,科属组成分析和与其它地区以共有属关系构建的相似性系数的谁知盘中计算,同时可大量节省研究人员的劳动强度和时间,对提高研究水平有一定的效果。     

1,25-双羟胆钙化醇及其受体含量检测的实验研究

 建立了一种改良的血清1,25-双羟胆钙化醇(1,25-Dihydroxycholecalciferol,DHCC)超微量放射受体检测(RRA)技术。完成了灵敏度、精密度、准确度、稳定性及特异性等技术指标。报告了我国健康青年血清DHCC正常值;检测了先天性佝偻病、青春期佝偻病病人及患肾性骨病奶牛等血清DHCC水平。 根据配体与受体相互结合的定量关系,建立了DHCCR(DHCC受体)检测技术。在游离与结合配基分离方面,除建立与比较了DCC(葡聚糖包埋的活性炭)及HAP(羟基磷灰石)方法外,还首次将IEF(等电聚焦电泳)应用于DHCCR分离技术。对佝偻病鸡小肠粘膜上皮细胞受体含量进行了检测并比较了鸡小肠、输卵管壳腺及肝组织DHCCR含量。     

转移及非转移肿瘤移植后615小鼠血液流变学变化的研究

血道高转移瘤株FC、淋巴合并血道高转移瘤株U14、淋巴道高转移瘤株H22、非转移瘤株P615分别接种于336只纯系近交615小鼠.不同时间取血并处死动物,进行组织学及血液流变学检查.将转移瘤发展过程分为潜伏期、侵袭期、转移早、中、晚期,非转移瘤发展过程分为潜优期、增殖期、囊腔形成期及中心坏死期.本实验结果显示,不同转移能力及途径肿瘤发展的不同时期血液流变学变化规律不同,因而表明肿瘤侵袭、转移与血液流变学变化之间存在互为因果的紧密关系.其临床诊断及治疗意义被讨论.     

五步蛇主要数量性状与排毒量的相关分析

本文首次报道了五步蛇身体主要部位的数量性状与其排毒量的关系。其中我们发现与排毒量有关的部位是头积、体粗、体重、佛指长。     

板栗干腐病的防治研究之五

研究证明,花期防治以及及时采收、低温贮藏和快速运输等保水措施是控制板栗干腐病的较为有效的措施。     

Triterpenoid saponins from Clinopodium polycephalum.

A new triterpenoid saponin, clinopodiside A, has been isolated from Clinopodium polycephalum. Its structure was established by spectroscopic methods and X-ray diffraction analysis as 3-O-beta-D-glucopyranosyl(1----6)-[ beta-D-glucopyranosyl (1----4)]-beta-D-glucopyranosyl-olean-11,13(18)-diene-3 beta,16 beta, 23,28-tetrol.     

Intrinsic fluorescence of the bacterial copper-containing protein amicyanin

The fluorescence properties of the single tryptophanyl residue present in amicyanin from Thiobacillus versutus are very similar to those of azurin from Pseudomonas aeruginosa and other mononuclear blue copper proteins. The emission maximum is well structured and centered at 318 nm. The quantum yield is strongly affected by the presence of copper, the removal of which is accompanied by a more than sixfold increase in fluorescence, without change in shape. The fluorescence decay of holo-amicyanin is heterogeneous with a longer component of 5.7 ns and a shorter one of 0.7 ns accounting for 90% of the total emitting molecules. Copper-free amicyanin shows instead a single exponential decay (3.3 ns) of intrinsic fluorescence. This lifetime decreases as the temperature increases as does the longer lifetime component of holoamicyanin.     

Acoustic modes and nonbonded interactions of the double helix

Observations of acoustic velocities in DNA fibers have been used to refine nonbonded force constants for the DNA double helix. Long-range forces are found to be needed for A conformation and are likely to dominate in B conformation as well. The acoustic dispersion curves are described and calculated. A correction due to the effects of water is calculated. The effect of nonbonded interaction on other vibrational modes is calculated.