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71.
72.
在烧伤大鼠创面接种绿脓杆菌(109CFU/ml,)使用银锌霜、磺胺嘧啶银霜,生态霜及空白霜膏抗感染实验,结果发现:生态霜作用与磺胺嘧啶银、银锌霜效果相当,既能拮抗绿脓杆菌感染,又对创面有保护作用,如先使用生态霜效果更好。 相似文献
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以长白山阔叶红松林为研究对象,根据Raupach提出的LocalizedNearField(LNF)理论为依据,耦合垂直速度标准差σw(z)和拉格朗日时间尺度TL(z),建立林冠内水汽源汇强度和平均浓度廓线之间的关系;利用拉格朗日逆分析法(inverseLagrangiandispersionanalysis,IL)提出了通过林冠水汽浓度梯度计算林冠内的水汽源汇强度进而推算森林蒸散的方法.最终得到的结果与开路涡动相关系统的观测数据比较显示,对于白天水汽累积通量的模拟精度达到87.3%;IL模拟值高于EC实测值,大约高出15%~25%;林冠白天水汽通量远大于夜间,占日水汽总通量的70%以上.6~8月的水汽白天累积总通量高于5月和9月. 相似文献
76.
Shouchun Cao Ying Zhang Feng Liu Qin Wang Quanfu Zhang Qinzhi Liu Chuan Li Mifang Liang Dexin Li 《Molecular biotechnology》2009,41(2):91-98
In order to further study the B subunit of the Escherichia coli heat-labile enterotoxin (LTB), we obtained the LTB gene from pathogenic E. coli, cloned it into the pET22b (+) prokaryotic expression vector, and expressed it as a fusion protein with His tag in E. coli BL21 (DE3). The recombinant LTB was expressed and purified by Ni2+ affinity chromatography. The biological activity of the purified recombinant LTB was assayed in a series of monosialotetrahexosylganglioside
(GM1)-ELISA experiments. The recombinant LTB (rLTB) was efficiently expressed under the induction of 10 g/l lactose at 37°C
for 6 h and yielded up to 31% of the total bacterial protein. Fused with pelB signal peptide, rLTB was successfully localized
to the periplasmic space. GM1-ELISA experiments showed that the rLTB obtained retains strong GM1 ganglioside-binding activity.
The ELISA result of hantavirus nucleoprotein-specific secretory immunoglobulin A (sIgA) and IgG showed that intranasal administration
of inactivated hantavirus with rLTB significantly increased the levels of hantavirus-specific sIgA (P < 0.01) and IgG (P < 0.01) in comparison with inactivated hatavirus alone. In summary, we have developed a method for the efficient secretory
expression and purification of rLTB, and the inactivated hantavirus co-administered intranasally with rLTB could effectively
induce both mucosal and humoral immune responses specific to hantavirus.
Shouchun Cao and Ying Zhang contributed equally to this work. 相似文献
77.
制备抗登革病毒NS1蛋白单克隆抗体,建立检测NS1的ELISA方法。表达1~4型登革病毒NS1蛋白,将1型NS1蛋白纯化后免疫BALB/c小鼠,通过杂交瘤技术制备单克隆抗体。经ELISA、Western blotting、间接免疫荧光筛选和鉴定单克隆抗体,进行纯化和HRP标记。通过鉴定每两株单抗之间是否存在竞争作用,选择非竞争单抗组合并建立NS1捕获法ELISA。结果获得7株高滴度抗NS1单抗,捕获法ELISA可以检出10ng/mL NS1。原核表达登革病毒NS1蛋白制备的单抗可以和天然病毒抗原反应,NS1捕获法ELISA可以用于登革病毒感染检测。 相似文献
78.
Yan Zhang Zhen Zhu Weizhong Yang Jun Ren Xiaojuan Tan Yu Wang Naiying Mao Songtao Xu Shuangli Zhu Aili Cui Yong Zhang Dongmei Yan Qun Li Xiaoping Dong Jing Zhang Yueping Zhao Junfeng Wan Zijian Feng Junling Sun Shiwen Wang Dexin Li Wenbo Xu 《Virology journal》2010,7(1):1-9
Molecular characterization of wild-type measles viruses in China during 1995-2004 demonstrated that genotype H1 was endemic and widely distributed throughout the country. H1-associated cases and outbreaks caused a resurgence of measles beginning in 2005. A total of 210,094 measles cases and 101 deaths were reported by National Notifiable Diseases Reporting System (NNDRS) and Chinese Measles Laboratory Network (LabNet) from 2006 to 2007, and the incidences of measles were 6.8/100,000 population and 7.2/100,000 population in 2006 and 2007, respectively. Five hundred and sixty-five wild-type measles viruses were isolated from 24 of 31 provinces in mainland China during 2006 and 2007, and all of the wild type virus isolates belonged to cluster 1 of genotype H1. These results indicated that H1-cluster 1 viruses were the predominant viruses circulating in China from 2006 to 2007. This study contributes to previous efforts to generate critical baseline data about circulating wild-type measles viruses in China that will allow molecular epidemiologic studies to help measure the progress made toward China's goal of measles elimination by 2012. 相似文献
79.
Yafei Feng Yi Liu Lin Wang Xiaoqing Cai Dexin Wang Kaimin Wu Hongli Chen Jia Li Wei Lei 《PloS one》2013,8(1)
Background
Clinical evidence indicates that late acute renal failure (ARF) predicts high mortality in severely burned patients but the pathophysiology of late ARF remains undefined. This study was designed to test the hypothesis that sustained reactive oxygen species (ROS) induced late ARF in a severely burned rat model and to investigate the signaling mechanisms involved.Materials and Methods
Rats were exposed to 100°C bath for 15 s to induce severe burn injury (40% of total body surface area). Renal function, ROS generation, tubular necrosis and apoptosis, and phosphorylation of MAPK and Akt were measured during 72 hours after burn.Results
Renal function as assessed by serum creatinine and blood urea nitrogen deteriorated significantly at 3 h after burn, alleviated at 6 h but worsened at 48 h and 72 h, indicating a late ARF was induced. Apoptotic cells and cleavage caspase-3 in the kidney went up slowly and turned into significant at 48 h and 72 h. Tubular cell ROS production shot up at 6 h and continuously rose during the 72-h experiment. Scavenging ROS with tempol markedly attenuated tubular apoptosis and renal dysfunction at 72 h after burn. Interestingly, renal p38 MAPK phosphorylation elevated in a time dependent manner whereas Akt phosphorylation increased during the first 24 h but decreased at 48 h after burn. The p38 MAPK specific inhibitor SB203580 alleviated whereas Akt inhibitor exacerbated burn-induced tubular apoptosis and renal dysfunction. Furthermore, tempol treatment exerted a duplex regulation through inhibiting p38 MAPK phosphorylation but further increasing Akt phosphorylation at 72 h postburn.Conclusions
These results demonstrate that sustained renal ROS overproduction induces continuous tubular cell apoptosis and thus a late ARF at 72 h after burn in severely burned rats, which may result from ROS-mediated activation of p38 MAPK but a late inhibition of Akt phosphorylation. 相似文献80.
Feng D Li L Yang F Tan W Zhao G Zou H Xian M Zhang Y 《Applied microbiology and biotechnology》2011,91(2):399-405
Pretreatment of cellulose with ionic liquids (ILs) can improve the efficiency of the hydrolysis by increasing the surface
area of the substrates accessible to solvents and cellulases. However, the IL methods are facing challenges to separate the
hydrolyzed sugar products as well as the renewable ILs from the complex hydrolysis mixtures. In this study, an alumina column
chromatography (ACC) method was developed for the separation of hydrophilic IL N-methyl-N-methylimidazolium dimethyl phosphate ([Mmim][DMP]) and glucose, which was the main ingredient of the monosaccharide hydrolyzate.
The processing parameters involved in ACC separation were investigated in detail. Our results showed that the recovery yields
of [Mmim][DMP] and glucose can reach up to 93.38% and 90.14%, respectively, under the optimized parameters: the sampling ratio
of 1:20 between the applied sample volume and the bed volume of the column; a gradient elution using methanol (100%, 150 ml)
and then water (170 ml) as eluents with 1 ml/min flow rate. The recovered [Mmim][DMP] showed qualified property and was effective
in a new hydrolysis reaction. In addition, scale-up ACC separations were successfully done with satisfied separation performance.
The results indicated that the ACC is one of the available methods for the separation of ILs and monosaccharides from the
hydrolysis mixtures. 相似文献