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61.
Francisco M. Costela Jorge Otero-Millan Michael B. McCamy Stephen L. Macknik Xoana G. Troncoso Ali Najafian Jazi Sharon M. Crook Susana Martinez-Conde 《PloS one》2014,9(10)
Our eyes move continuously. Even when we attempt to fix our gaze, we produce “fixational” eye movements including microsaccades, drift and tremor. The potential role of microsaccades versus drifts in the control of eye position has been debated for decades and remains in question today. Here we set out to determine the corrective functions of microsaccades and drifts on gaze-position errors due to blinks in non-human primates (Macaca mulatta) and humans. Our results show that blinks contribute to the instability of gaze during fixation, and that microsaccades, but not drifts, correct fixation errors introduced by blinks. These findings provide new insights about eye position control during fixation, and indicate a more general role of microsaccades in fixation correction than thought previously. 相似文献
62.
Mariano De Figueroa Taboada 《BMJ (Clinical research ed.)》1954,1(4859):437-438
63.
Francisco M. Costela Jorge Otero-Millan Michael B. McCamy Stephen L. Macknik Leandro L. Di Stasi Héctor Rieiro John R. Leigh Xoana G. Troncoso Ali Najafian Jazi Susana Martinez-Conde 《PloS one》2015,10(6)
Saccadic intrusions (SIs), predominantly horizontal saccades that interrupt accurate fixation, include square-wave jerks (SWJs; the most common type of SI), which consist of an initial saccade away from the fixation target followed, after a short delay, by a return saccade that brings the eye back onto target. SWJs are present in most human subjects, but are prominent by their increased frequency and size in certain parkinsonian disorders and in recessive, hereditary spinocerebellar ataxias. SWJs have been also documented in monkeys with tectal and cerebellar etiologies, but no studies to date have investigated the occurrence of SWJs in healthy nonhuman primates. Here we set out to determine the characteristics of SWJs in healthy rhesus macaques (Macaca mulatta) during attempted fixation of a small visual target. Our results indicate that SWJs are common in healthy nonhuman primates. We moreover found primate SWJs to share many characteristics with human SWJs, including the relationship between the size of a saccade and its likelihood to be part of a SWJ. One main discrepancy between monkey and human SWJs was that monkey SWJs tended to be more vertical than horizontal, whereas human SWJs have a strong horizontal preference. Yet, our combined data indicate that primate and human SWJs play a similar role in fixation correction, suggesting that they share a comparable coupling mechanism at the oculomotor generation level. These findings constrain the potential brain areas and mechanisms underlying the generation of fixational saccades in human and nonhuman primates. 相似文献
64.
Corkidi G Taboada B Wood CD Guerrero A Darszon A 《Biochemical and biophysical research communications》2008,373(1):125-129
Sperm motility, crucial for fertilization, has been mostly studied in two dimensions (2D) by recording their swimming trajectories near a flat surface. However, spermatozoa swim in three-dimensions (3D) to find eggs, with their speed being the main impediment to track them under realistic conditions. Here, we describe a novel method allowing 3D tracking and analysis of the trajectories of multiple free-swimming sperm. The system uses a piezo-electric device displacing a large focal distance objective mounted on a microscope to acquire 70 image stacks per second, each stack composed of 60 images that span a depth of 100 μm. With this method, 3D paths of multiple sperm in the same field could be visualized simultaneously during 1 s . Within the same sample we found that surface-confined sperm swam 25% slower, produced 3-fold fewer circular revolutions per second, and had trajectories of 134% greater radius of curvature than those sperm swimming freely in 3D. 相似文献
65.
The transportation of hazardous goods by road implies a risk for both humans and the environment, in that an accident involving a vehicle transporting this kind of material may cause extensive material and environmental damage and might even endanger lives. For this reason, both public and private entities (e.g., insurance companies) have a growing interest in studies that assess the risks associated with hazardous goods transportation This article describes a method for calculating these risks. The risk is determined on the basis of a calculation of the probability of the occurrence of an accident and the gravity of the damage, which is in turn a function of the potential damage inherent in the goods being transported taken with the vulnerability of the environmental medium in which the accident takes place. The mathematical model proposed is easily implemented in a geographical information system that will produce risk maps delimiting the more potentially conflictive stretches of roadway. 相似文献
66.
Carlos Rosas Gerard CuzonGabriela Gaxiola Cristina PascualGabriel Taboada Leticia ArenaAlain van Wormhoudt 《Journal of experimental marine biology and ecology》2002,268(1):47-67
We are reporting results directed to explain the relation between carbohydrates (CHO), protein metabolism, and the energetic balance of Litopenaeus vannamei juveniles. The interaction of dietary CHO and salinity was measured to try to understand the relation between osmotic control and metabolism, both from a biochemical and energetic point of view. Two experiments were done. In the first experiment, shrimp were fed with 0%, 5%, 33%, and 61% CHO and maintained at 15‰ and 40‰ salinity. Glucose, lactate protein, hemocyanin, ammonia concentration, and osmotic pressure were measured in blood. Digestive gland glycogen (DGG) was measured also. In the second experiment, shrimp were fed with 0% and 38% dietary CHO and maintained at 15‰ and 40‰ salinity. From that shrimp, absorbed energy (Abs) was calculated as: Abs=respiration (R)+ammonia excretion (U) and production (P); assimilated energy (As) was calculated as the product of R×P. Osmotic pressure, hemocyanin, protein, lactate, and blood ammonia increased with the reduction in dietary CHO. In contrast, an increase in blood glucose was observed with an increase in dietary CHO. Digestive gland glycogen (DGG) increased following a saturation curve with a DGG maximum at 33% dietary CHO. Blood metabolites of fasting and feeding shrimp showed the same behavior. Energy balance results showed that shrimp maintained in low salinity and fed without CHO waste more energy in U production than for shrimp maintained in high salinity and fed with high CHO levels. Notwithstanding, the production efficiency was higher in shrimp fed without CHO than that observed in shrimp fed with high CHO independent of salinity. A scheme trying to integrate the relation between CHO and protein metabolism and the way in which both are modulated by salinity is presented. From published and present results, there are two factors that apparently control the use of high dietary CHO levels; α-amylase enzyme-dietary CHO level capacity and glycogen saturation in DG. Production of glucose is limited in shrimp because of saturation of α-amylase when shrimp are fed with diets above 33% CHO. This is the first control point of starch metabolism. The digestive gland is saturated with glycogen in shrimp fed with dietary CHO levels >33%. This is apparently the second control point of CHO metabolism that limits growth rate in such conditions. The high metabolic cost related to high CHO diets could explain why shrimp are well adapted to use protein as a source of energy. 相似文献
67.
Céline Labrune Antoine Grémare Jean-Michel Amouroux Rafael Sardá João Gil Sergi Taboada 《Helgoland Marine Research》2008,62(3):201-214
Samples of soft-sediment macrobenthos from 92 sites between 10 and 50 m depth were used to assess (1) the main soft-bottom
macrofauna communities in the Gulf of Lions, (2) the different components of the diversity of benthic macrofauna in this area,
and (3) the relevance of the use of major taxonomic groups as surrogates for the analysis of the structure and diversity of
total macrofauna. Three main communities were identified by cluster analysis and associated procedures. These communities
corresponded well to the assemblages recently identified on the basis of polychaete composition. The α-diversity indices were
in accordance with those reported for similar communities in the Mediterranean. Conversely, the β-diversity value was higher
than the few other data available in the literature for marine soft-bottom macrofauna. The total number of species in the
studied area estimated by the “total species accumulation curve” (TS) method was 2,319, which was only 10% higher than the
number obtained by extrapolation of the species–area curve. The similarity matrix based on polychaetes correlated best with
the one based on total macrofauna. Polychaetes and crustaceans were also the best surrogates of total macrofauna when assessing
α-diversity (except in the case of Δ*). Conversely, molluscs were the best surrogates of total macrofauna β-diversity. Our
results show that the choice of an optimal surrogate for total benthic macrofauna depends on the characteristic of the benthic
macrofauna to be studied. Moreover, this choice is also dependent on the environment to be studied. 相似文献
68.
Angela Taboada D. Johan Kotze Reyes Trrega Jos M. Salgado 《Basic and Applied Ecology》2008,9(2):161-171
We studied the responses of carabid beetles to Pinus sylvestris ageing in four plantations aged 2–3, 10–12, 40–45 and 80 years, and one mature natural pine forest – in NW Spain. In 2003, a total of 16,866 carabid individuals (50 species) were collected using pitfall traps. Overall carabid abundance and species richness differed significantly among the five age classes with the highest values occurring in the youngest age class. The carabid assemblages of the initial stages of the ageing sequence differed considerably from the later stages that were relatively similar to each other – a consequence of differences in species dominance at specific age classes. Yet, the carabid assemblage of the 80-year-old stage did not approach that of the natural pine forest, but, unexpectedly, the 40–45-year-old age class did. Canopy cover and soil pH were the main environmental variables that affected the carabid distribution. We also detected species-level responses to the ageing process, with open habitat species more abundantly collected at the youngest stages and forest specialists at the older stages. We conclude that carabid beetles responded predictably to the pine plantation ageing process, and that although pine forests in NW Spain do not seem to provide habitat for unique species, they act as secondary habitats for forest specialist species. 相似文献
69.
Albaiceta GM Gutiérrez-Fernández A Parra D Astudillo A García-Prieto E Taboada F Fueyo A 《American journal of physiology. Lung cellular and molecular physiology》2008,294(3):L535-L543
Matrix metalloproteinase-9 (MMP-9) is released by neutrophils at the sites of acute inflammation. This enzyme modulates matrix turnover and inflammatory response, and its activity has been found to be increased after ventilator-induced lung injury. To clarify the role of MMP-9, mice lacking this enzyme and their wild-type counterparts were ventilated for 2 h with high- or low-peak inspiratory pressures (25 and 15 cmH2O, respectively). Lung injury was evaluated by gas exchange, respiratory mechanics, wet-to-dry weight ratio, and histological analysis. The activity of MMP-9 and levels of IL-1beta, IL-4, and macrophage inflammatory protein (MIP-2) were measured in lung tissue and bronchoalveolar lavage fluid (BALF). Cell count and myeloperoxidase activity were measured in BALF. There were no differences between wild-type and Mmp9-/- animals after low-pressure ventilation. After high-pressure ventilation, wild-type mice exhibited an increase in MMP-9 in tissue and BALF. Mice lacking MMP-9 developed more severe lung injury than wild-type mice, in terms of impaired oxygenation and lung mechanics, and higher damage in the histological study. These effects correlated with an increase in both cell count and myeloperoxidase activity in the BALF, suggesting an increased neutrophilic influx in response to ventilation. An increase in IL-1beta and IL-4 in the BALF only in knockout mice could be responsible for the differences. There were no differences between genotypes in MMP-2, MMP-8, or tissue inhibitors of metalloproteinases. These results show that MMP-9 protects against ventilator-induced lung injury by decreasing alveolar neutrophilic infiltration, probably by modulation of the cytokine response in the air spaces. 相似文献
70.
Ziebell K Steele M Zhang Y Benson A Taboada EN Laing C McEwen S Ciebin B Johnson R Gannon V 《Applied and environmental microbiology》2008,74(14):4314-4323
In this study, the association between genotypic and selected phenotypic characteristics was examined in a collection of Canadian Escherichia coli O157:H7 strains isolated from humans and cattle in the provinces of Alberta, Ontario, Saskatchewan, and Quebec. In a subset of 69 strains selected on the basis of specific phage types (PTs), a strong correlation between the lineage-specific polymorphism assay (LSPA6) genotype and PT was observed with all strains of PTs 4, 14, 21, 31, 33, and 87 belonging to the LSPA6 lineage I (LSPA6-LI) genotype, while those of PTs 23, 45, 67, and 74 belonged to LSPA6 lineage II (LSPA6-LII) genotypes. This correlation was maintained when additional strains of each PT were tested. E. coli O157:H7 strains with the LSPA6-LI genotype were much more common in the collection than were the LSPA6-LII or lineage I/II (LSPA6-LI/II)-related genotypes (82.6, 11.2, and 5.8%, respectively). Of the strains tested, proportionately more LSPA6-LI than LSPA6-LII genotype strains were isolated from humans (52.7% versus 19.7%) than from cattle (47.8% versus 80.2%). In addition, 96.7% of the LSPA6-LII strains carried the stx(2c) variant gene, while only 50.0% of LSPA6-LI/II and 2.7% of LSPA6-LI strains carried this gene. LSPA6-LII strains were also significantly more likely to possess the colicin D gene, cda (50.8% versus 23.2%), and have combined resistance to streptomycin, sulfisoxazole, and tetracycline (72.1% versus 0.9%) than were LSPA6-LI strains. The LSPA6 genotype- and PT-related characteristics identified may be important markers of specific ecotypes of E. coli O157:H7 that have unique epidemiological and virulence characteristics. 相似文献