New Lithium Salt Forms Interphases Suppressing Both Li Dendrite and Polysulfide Shuttling

Lithium–sulfur batteries (LSBs) are considered promising candidates for the next‐generation energy‐storage systems due to their high theoretical capacity and prevalent abundance of sulfur. Their reversible operation, however, encounters challenges from both the anode, where dendritic and dead Li‐metal form, and the cathode, where polysulfides dissolve and become parasitic shuttles. Both issues arise from the imperfection of interphases between electrolyte and electrode. Herein, a new lithium salt based on an imide anion with fluorination and unsaturation in its structure is reported, whose interphasial chemistries resolve these issues simultaneously. Lithium 1, 1, 2, 2, 3, 3‐hexafluoropropane‐1, 3‐disulfonimide (LiHFDF) forms highly fluorinated interphases at both anode and cathode surfaces, which effectively suppress formation of Li‐dendrites and dissolution/shuttling of polysulfides, and significantly improves the electrochemical reversibility of LSBs. In a broader context, this new Li salt offers a new perspective for diversified beyond Li‐ion chemistries that rely on a Li‐metal anode and active cathode materials.     

An auto-inducible expression and high cell density fermentation of Beefy Meaty Peptide with Bacillus subtilis

Bioprocess and Biosystems Engineering - Currently, some cases about the expression of flavor peptides with microorganisms were reported owing to the obvious advantages of biological expression over...     

Economical production of isomaltulose from agricultural residues in a system with sucrose isomerase displayed on Bacillus subtilis spores

Bioprocess and Biosystems Engineering - A safe, efficient, environmentally friendly process for producing isomaltulose is needed. Here, the biocatalyst, sucrose isomerase (SIase) from Erwinia...     

小黑杨PsnHB13基因在烟草中的遗传转化与功能分析

克隆小黑杨HD-ZIP家族基因PsnHB13,对该基因进行生物信息学分析、过表达载体构建、烟草的遗传转化。结果表明小黑杨PsnHB13基因cDNA全长870 bp,编码289个氨基酸。成功构建植物过表达载体pROKⅡ-PsnHB13,并通过农杆菌介导的叶盘法将外源基因转入野生型烟草。检测结果显示PsnHB13已成功整合入烟草基因组中,并在mRNA水平表达。通过观察转基因烟草的生长,发现过表达PsnHB13基因的烟草与野生型相比出现叶面积变小、叶型变长,根系生长缓慢,花朵变小等明显表型。说明PsnHB13基因主要对烟草叶片、根系及花的生长发育起到负调控作用。本文为研究PsnHB13基因对杨树生长发育的影响提供理论基础。     

汉黄芩素通过调控上皮间质转化抑制胃癌细胞的侵袭转移

目的:汉黄芩素是中药黄芩中的一种黄酮,具有体内外抗癌活性。然而,汉黄芩素对人胃癌细胞的作用尚不十分清楚。本研究拟探讨汉黄芩素对人胃癌细胞MGC-803侵袭转移能力的影响及其对上皮间质转化(Epithelial to Mesenchymal Transition,EMT)的作用机制。方法:采用MTT法测定汉黄芩素对人胃癌细胞MGC-803增殖能力的影响,通过划痕实验、Transwell试验检测汉黄芩素对人胃癌细胞MGC-803迁移、侵袭能力的影响。通过免疫印迹法和免疫荧光法分析汉黄芩素对EMT的影响。结果:20μM以上浓度的汉黄芩素能抑制人胃癌细胞MGC-803的增殖,不同浓度的汉黄芩素能抑制人胃癌细胞MGC-803的迁移和侵袭,且呈浓度依赖性。此外,汉黄芩素能抑制间质标记蛋白波形蛋白(Vimentin)和锌指蛋白E-盒结合同源异形盒-1(ZEB1)的表达,促进上皮标记蛋白E-钙黏蛋白(E-cadherin)的表达。结论:汉黄芩素能抑制胃癌细胞的侵袭和迁移,这一作用可能与其抑制EMT的发生有关。     

基于磁驱动技术的空肠营养管的设计

目的:为解决目前床旁鼻肠管快速置入成功率低这一临床难题,该文提出一种基于磁驱动技术的空肠营养管的设计。方法:分析了现有空肠营养管置管过程中的动力因素,结合磁驱动技术原理,提出了通过体外旋转磁场带动体内感应磁体螺旋式前进的设计方案,以期缩短空肠营养管的飘管时间。结果:该设计包括磁性空肠营养管和体外磁力驱动装置两部分。其中磁性空肠营养管由管体和感应磁头两部分构成,感应磁头包括磁体内核和硅胶外壳。磁力驱动装置由多极磁体和手持式微型电机组成。操作时通过体外磁力驱动装置发出大旋转磁场带动空肠营养管的感应磁头做轴向旋转运动,可加速空肠营养管在肠道内的移动,达到缩短飘管时间的目的。结论:该设计基于磁驱动技术原理,设计巧妙,符合磁力学原理,操作简单,具有临床应用潜力。     

1-6岁儿童维生素D与年龄、性别、季节及体质量指数的关系研究

目的:探讨1-6岁儿童维生素D与年龄、性别、季节及体质量指数(BMI)的关系,为临床有效指导维生素D的补充提供参考依据。方法:选取2000年1月～2019年1月于我院接受体检的1～6岁儿童816例作为研究对象。采用电化学发光法检测所有研究对象的血清25-羟维生素D[25(OH)D]水平,并分析血清25(OH)D水平与儿童年龄、性别、BMI以及季节的关系。结果:816例儿童维生素D营养不足和缺乏人数占比为14.83%。1～3岁儿童血清25(OH)D水平高于3～6岁儿童(P0.05)。男童血清25(OH)D水平高于女童(P0.05)。肥胖儿童血清25(OH)D水平低于正常与超重儿童,且超重儿童血清25(OH)D水平低于正常儿童(均P0.05)。春、夏季儿童血清25(OH)D水平均高于秋、冬季儿童(均P0.05)。结论:1～6岁儿童的维生素D营养状况不容乐观,随着年龄的增长儿童血清25(OH)D水平显著降低,且男童高于女童,春、夏季高于秋、冬季。临床工作可通过增加其户外活动,继而达到改善维生素D营养状况的目的。     

空心钉加内侧支撑钢板与单纯空心钉治疗PauwelsⅢ型股骨颈骨折的疗效比较研究

目的:比较PauwelsⅢ型股骨颈骨折分别经单纯空心钉、空心钉加内侧支撑钢板治疗的临床疗效。方法:回顾性分析2016年3月～2019年2月期间收治的113例PauwelsⅢ型股骨颈骨折患者的临床资料,根据手术方式分为A组(n=55,单纯空心钉内固定治疗)和B组(n=58,空心钉加内侧支撑钢板治疗),比较两组患者围术期指标、髋关节功能、术后疼痛及复位质量,记录两组患者随访期间并发症发生情况。结果:B组术中出血量多于A组,手术时间长于A组(均P0.05);B组骨折愈合时间、完全负重时间短于A组(P0.05)。两组患者术后3个月、术后6个月髋关节Harrris评分呈升高趋势,视觉模拟评分量表(VAS)评分呈下降趋势(P0.05);B组术后3个月、术后6个月髋关节Harrris评分高于A组,VAS评分则低于A组(P0.05)。与术后3 d相比,A组患者术后6个月正位、侧位Garden指数降低(P0.05);B组术后6个月正位、侧位Garden指数评分高于A组(P0.05)。B组并发症发生率低于A组(P0.05)。结论:与单纯空心钉内固定治疗PauwelsⅢ型股骨颈骨折相比,空心钉加内侧支撑钢板虽然术中出血量多,手术时间略长,但其术后恢复效果更佳,且并发症发生率更低,临床应用价值较高。     

Epidemiological,Clinical and Serological Characteristics of Children with Coronavirus Disease 2019 in Wuhan: A Single-centered,Retrospective Study

In December 2019, SARS-CoV-2 was first detected in the samples obtained from three adult patients who suffered from an unknown viral pneumonia in Wuhan (Li et al. 2020). This unknown viral pneumonia is further named as coronavirus disease 2019 (COVID-19) by the World Health Organization. To date, the number of new COVID-19 cases has continued to skyrocket and the impact of SARS-CoV-2 on humans is far greater than any pathogen of this century in both breadth and depth. Previous studies have shown that adults with COVID-19 have symptoms of fever, dry cough, dyspnea, fatigue and lymphocytopenia. Moreover, COVID-19 is more likely to cause death in the elderly, especially those with chronic comorbidities (Huang et al. 2020). In Wuhan, more than 50, 000 COVID-19 cases have been confirmed, including over 780 pediatric patients, and only one child death case (Lu et al. 2020). Although the number of children cases was far fewer than that of adults, COVID-19 might endanger children's health and the information on children remains limited, especially in serological study. In the retrospective study, the investigators analyzed the epidemiological, clinical and serological characteristics of children with COVID-19 in Wuhan in the early stages of the outbreak, which might provide theoretical and practical help in controlling COVID-19 and similar emerging infectious diseases in the future.