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91.
Deng Ling Jiang Jin Chen Sha Lin Xing Zuo Tianrui Hu Qingwen Wu Yu Fan Xiaomei Dong Zhi 《Neurochemical research》2022,47(7):2002-2015
Neurochemical Research - The aim of this study was to investigate the role and underlying mechanism of the long non-coding RNA ANRIL (antisense noncoding RNA in the INK4 locus, ANRIL) in ischemia... 相似文献
92.
Yanjie Chen Xinzhao Zuo Qinglv Wei Jie Xu Xiaoyi Liu Shiling Liu Haocheng Wang Qingya Luo Yuya Wang Yu Yang Hongyan Zhao Jing Xu Tao Liu Ping Yi 《International journal of biological sciences》2023,19(2):691
Cervical cancer (CC) is one of the most common gynecological malignancies with poor prognosis for advanced CC patients. LRRC8A is a volume-regulated anion channel protein involved in cellular homeostasis, but its role in CC remains largely unknown. In this study, we found that LRRC8A is elevated in CC and associated with poor prognosis. LRRC8A maintains cell survivals under the hypotonic condition, and promotes tumorigenesis through apoptosis suppression in vitro and in vivo. Notably, LRRC8A is upregulated by NSUN2-mediated m5C modification. m5C modified-LRRC8A mRNA is bound by the RNA binding protein YBX1 followed by the increased RNA stability. Moreover, loss of NSUN2 suppresses the proliferation and metastasis of CC cells, and NSUN2 expression is positively correlated with LRRC8A expression in CC. Altogether, our study demonstrates that the NSUN2-m5C-LRRC8A axis is crucial and would be a potential therapeutic target for CC. 相似文献
93.
Xin Wang Yu-Ting Dong Xiu-Ming Hu Ji-Zhou Zhang Nan-Rui Shi Yan-Qin Zuo Xu Wang 《Purinergic signalling》2023,19(1):283
Extracellular ATP is a potent signaling molecule released from various cells throughout the body and is intimately involved in the pathophysiological functions of the nervous system and immune system by activating P2 purinergic receptors. Recent increasingly studies showed that extracellular ATP exhibits circadian oscillation with an approximately 24-h periodicity, which participates in regulatory pathways of central oscillator suprachiasmatic nucleus and peripheral oscillator bladder, respectively. Oscillators modulate the protein expression of ATP release channels and ectonucleotidase activity through clock genes; indeed, real-time alterations of ATP release and degradation determine outcomes of temporal character on extracellular ATP rhythm. The regulatory pathways on extracellular ATP rhythm are different in central and peripheral systems. In this review, we summarize the circadian rhythm of extracellular ATP and discuss several circadian regulatory pathways in different organs via ATP release and degradation, to provide a new understanding for purinergic signaling in the regulatory mechanism of circadian rhythm and a potential target to research the circadian regulation of extracellular ATP in other circadian oscillators. 相似文献
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Tetsuji Yamashita Pierre Hakizimana Siva Wu Ahmed Hassan Stefan Jacob Jamshid Temirov Jie Fang Marcia Mellado-Lagarde Richard Gursky Linda Horner Barbara Leibiger Sara Leijon Victoria E. Centonze Per-Olof Berggren Sharon Frase Manfred Auer William E. Brownell Anders Fridberger Jian Zuo 《PLoS genetics》2015,11(9)
Nature’s fastest motors are the cochlear outer hair cells (OHCs). These sensory cells use a membrane protein, Slc26a5 (prestin), to generate mechanical force at high frequencies, which is essential for explaining the exquisite hearing sensitivity of mammalian ears. Previous studies suggest that Slc26a5 continuously diffuses within the membrane, but how can a freely moving motor protein effectively convey forces critical for hearing? To provide direct evidence in OHCs for freely moving Slc26a5 molecules, we created a knockin mouse where Slc26a5 is fused with YFP. These mice and four other strains expressing fluorescently labeled membrane proteins were used to examine their lateral diffusion in the OHC lateral wall. All five proteins showed minimal diffusion, but did move after pharmacological disruption of membrane-associated structures with a cholesterol-depleting agent and salicylate. Thus, our results demonstrate that OHC lateral wall structure constrains the mobility of plasma membrane proteins and that the integrity of such membrane-associated structures are critical for Slc26a5’s active and structural roles. The structural constraint of membrane proteins may exemplify convergent evolution of cellular motors across species. Our findings also suggest a possible mechanism for disorders of cholesterol metabolism with hearing loss such as Niemann-Pick Type C diseases. 相似文献
96.
Juan Wang Wen-Yuan Gao Jian Zhang Bei-Mei Zuo Li-Ming Zhang Lu-Qi Huang 《In vitro cellular & developmental biology. Plant》2012,48(1):107-112
Based on the results of carbon source consumption in cell suspension culture of Panax quinquefolium L., 30 g L−1 sucrose was fed into a 5-L stirred tank bioreactor on day 16 of culture to enhance cell density and metabolite production.
Using a fed-batch cultivation strategy, polysaccharide production was enhanced to 1.608 g L−1, which was 1.96-fold greater than with batch cultivation. The maximum saponin yield (7.828 mg L−1) was obtained on day 24 and was about 36% higher than the yields obtained using batch cultivation. In a two-stage culture
process, a combined treatment with sucrose, lactoalbumin hydrolysate, and methyl jasmonate caused a significant increase in
total saponin yield (31.52 mg L−1) in cell cultures after 27 d. This value represents an increase of 4.03-fold compared with the total saponin yield in fed-batch
cultivation. The two-stage culture mode provided the best method for the in vitro production of secondary metabolites from P. quinquefolium. 相似文献
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100.
Ren Zhihua He Hongyi Fan Yu Chen Changhao Zuo Zhicai Deng Junliang 《Biological trace element research》2019,187(1):273-280
Biological Trace Element Research - Aflatoxin B1 (AFB1) can cause hepatotoxicity, genotoxicity, and immunosuppressive effects for a variety of organisms. Selenium (Se), as an essential nutrient... 相似文献