The Prevention Effect of Bacillus subtilis on Escherichia coli–Induced Mastitis in Mice by Suppressing the NF-κB and MAPK Signaling Pathways

Probiotics and Antimicrobial Proteins - Mastitis, common inflammation of the mammary gland, caused by various factors, is a challenge for the dairy industry. Escherichia coli (E. coli), a...     

河南济源晚石炭世太原组的一种复体四射珊瑚

Ａｃｒｏｃｙａｔｈｕｓ是一类块状体或丛状体的四射珊瑚，在北美分布于下石炭统，我国见于上石炭统，但丛状体的在我国系首次发现。在连续切片上，可见两个生长阶段，其繁殖方式有侧芽繁殖和边缘泡沫板内繁殖，系统分类上另置独立的科级分类。根据形态分析，该珊瑚栖息在动水，能量略高及食料丰富的浅水环境。当前标本为一新种：Ａ．ｊｉｙｕａｎｅｎｓｉｓｓｐ．ｎｏｖ．。     

Characterization of the 16S rRNA- and membrane-binding domains of Streptococcus pneumoniae Era GTPase: structural and functional implications.

Era is a highly conserved GTPase essential for bacterial growth. The N-terminal part of Era contains a conserved GTPase domain, whereas the C-terminal part of the protein contains an RNA- and membrane-binding domain, the KH domain. To investigate whether the binding of Era to 16S rRNA and membrane requires its GTPase activity and whether the GTPase domain is essential for these activities, the N- and C-terminal parts of the Streptococcus pneumoniae Era - Era-N (amino acids 1-185) and Era-C (amino acids 141-299), respectively - were expressed and purified. Era-C, which had completely lost GTPase activity, bound to the cytoplasmic membrane and 16S rRNA. In contrast, Era-N, which retained GTPase activity, failed to bind to RNA or membrane. These results therefore indicate that the binding of Era to RNA and membrane does not require the GTPase activity of the protein and that the RNA-binding domain is an independent, functional domain. The physiological effects of the overexpression of Era-C were assessed. The Escherichia coli cells overexpressing Era and Era-N exhibited the same growth rate as wild-type E. coli cells. In contrast, the E. coli cells overexpressing Era-C exhibited a reduced growth rate, indicating that the overexpression of Era-C inhibits cell growth. Furthermore, overexpression of era-N and era-C resulted in morphological changes. Finally, purified Era and Era-C were able to bind to poly(U) RNA, and the binding of Era to poly(U) RNA was significantly inhibited by liposome, as the amount of Era bound to the RNA decreased proportionally with the increase of liposome in the assay. Therefore, this study provides the first biochemical evidence that both binding sites are overlapping. Together, these results indicate that the RNA- and membrane-binding domain of Era is a separate, functional entity and does not require the GTPase activity or the GTPase domain of the protein for activity.     

猪精子中与卵透明带糖蛋白ZP3结合的蛋白质

依次经ＰＳＬ－Ｓｅｐｈａｒｏｓｅ亲和层析柱和纤维素ＣＭ－５２离子交换层析柱,从猪精子的ＣＨＡＰＳ抽提液分离得４个蛋白质组分。用固相透明带精蛋白结合试验（ＩＺＰＧＢＡ）检测;表明精子蛋白ＳＰ１和ＳＰ２具有结合透明带糖蛋白ＺＰ３的活性,ＳＰ２并显示凝集血球的活性。精子蛋白ＳＰ１与卵预温育明显抑制精卵结合,抑制活性与加入的精子蛋白的浓度呈正相关。用生物素标记的ＺＰ３和蛋白质印迹技术,证明ＳＰ１中的６８ｋＤ精子蛋白与ＺＰ３结合,提示６８ｋＤ精子蛋白参与精卵结合。     

Intergeneric hybridization between Pleurotus ostreatus and Schizophyllum commune by PEG-induced protoplast fusion

Intergeneric hybridization between Pleurotus ostreatus and Schizophyllum commune was studied using PEG-induced fusion. The fusion of protoplasts from auxotrophic mutant strains resulted in the formation of fusion hybrids in the frequencies of 3.6 to 7.3×10^–5. Most of these fusion hybrids were monokaryotic and sterile and no heterokaryosis occurred. Most fusants showed a significantly higher nuclear DNA content when compared to parental strains and no diploids (parent 1 genome plus parent 2 genome) were found. Some fusion hybrids revealed both parental fragments in nuclear and mitochondrial rDNA PCR profiles. AP-PCR (Arbitrarily-primed Polymerase Chain Reaction) fingerprints also indicated that most of the fusion products were recombinant hybrids.     

小麦返白系与不同基因型小麦品种杂交后代IPO表达的研究

以小麦返白系和对照矮变１号以及返白系与各不同生态类型的冬性、半冬性、春性小麦的杂交、回交Ｆ１、Ｆ２代为材料,研究这些不同基因型品种在返白期间过氧化物酶同工酶（ＩＰＯ）基因表达模式的动态变化特点。结果表明,在返白期间以返白系为母本的各杂交、回交品种的白化苗中,ＩＰＯ的个别酶分子表现了可逆的基因阻遏和去阻遏的表达现象。这种表达特点在正、反杂交后Ｆ１代中表现一致,从遗传模式上分别属于质－核互作型的分子遗     

栝楼核糖核酸酶在RNA序列分析中的应用

栝楼核糖核酸酶（RNase TCS）对U碱基具有高度的专一性,在无脲、pH3.5、50℃时,它几乎都在-NP ↓ U-处裂解RNA.它与RNase T1,U₂和有限的碱水解一起,可用于直接的酶法RNA序列分析．     

一种具有纤溶活性的枯草杆菌蛋白激酶的研究──Ⅰ高产酶菌株的筛选与鉴定

本文主要阐述了一种具有纤溶活性的枯草杆菌（Ｂａｃｉｌｌｕｓｓｕｂｔｉｌｉｓ）蛋白激酶产生菌株的筛选与鉴定的研究结果。作者从初筛的１２株Ｂａｃｉｌｌｕｓｓｕｂｌｉｌｉｓ菌中,通过对固体发酵和液体发酵所产生的枯草杆菌蛋白激酶,用琼脂糖－纤维蛋白平板法测其活性,经比较不同菌株的活性,筛选出两株高产酶菌株：Ｂ．ｓｕｂｔｉｌｉｓＨＷ—１２和Ｂ．ｓｕｂｔｉｌｉｓＨＷ—３。同时对菌体和菌落形态特点、生理生化反应进行了鉴定,认为Ｂ．ＳｕｂｔｉｌｉｓＨＷ－１２菌株可用来做为发酵生产该酶的菌种。     

迷宫漏斗蛛Agelena labyrinthica的染色体(蜘蛛目:漏斗蛛科)

报告迷宫漏斗蛛的染色体数目、形态结构和性染色体组成。结果表明,迷宫漏斗蛛体细胞染色体数目是:雄性2n=42,雌性2n=44,性别决定机制属X_1X_2O型。X染色体是全部染色体中最长的和最短的2个(对)。染色体似乎均为端或亚端着丝粒染色体,这由C-显带标本分析所证实,没有亚中着丝粒染色体的证据。C-带标本分析表明,常染色体中,各条染色体的C-带纹,其大小和染色深浅无明显差异,但X_2染色体上有明显宽的C-带纹。C-显带处理没有得到稳定的有重复性的带纹。     

朱砂叶螨羧酸脂酶最优测试条件的选择

应用二次回归通用旋转组合设计,对朱砂叶螨离体羧酸酯酶测试过程中所需缓冲液pH值、恒温时间、反应温度及底物浓度,设立4因子5水平试验,在考虑4个因子主效应和互作效应的情况下,筛选测试羧酸酯酶的最优条件组合。