全文获取类型
收费全文 | 49014篇 |
免费 | 17162篇 |
国内免费 | 2747篇 |
出版年
2024年 | 67篇 |
2023年 | 329篇 |
2022年 | 863篇 |
2021年 | 1742篇 |
2020年 | 3016篇 |
2019年 | 4766篇 |
2018年 | 4811篇 |
2017年 | 4844篇 |
2016年 | 5096篇 |
2015年 | 5373篇 |
2014年 | 5445篇 |
2013年 | 5846篇 |
2012年 | 3881篇 |
2011年 | 3415篇 |
2010年 | 4259篇 |
2009年 | 2870篇 |
2008年 | 1878篇 |
2007年 | 1343篇 |
2006年 | 1176篇 |
2005年 | 1119篇 |
2004年 | 1031篇 |
2003年 | 1013篇 |
2002年 | 909篇 |
2001年 | 752篇 |
2000年 | 620篇 |
1999年 | 488篇 |
1998年 | 227篇 |
1997年 | 211篇 |
1996年 | 180篇 |
1995年 | 156篇 |
1994年 | 141篇 |
1993年 | 114篇 |
1992年 | 129篇 |
1991年 | 103篇 |
1990年 | 99篇 |
1989年 | 104篇 |
1988年 | 68篇 |
1987年 | 53篇 |
1986年 | 30篇 |
1985年 | 48篇 |
1984年 | 31篇 |
1983年 | 40篇 |
1982年 | 33篇 |
1981年 | 21篇 |
1980年 | 20篇 |
1979年 | 26篇 |
1978年 | 23篇 |
1977年 | 13篇 |
1976年 | 16篇 |
1973年 | 19篇 |
排序方式: 共有10000条查询结果,搜索用时 46 毫秒
222.
223.
鳗鱼肌肉的氨基酸及营养价值 总被引:7,自引:1,他引:6
孔晓荣 《氨基酸和生物资源》1995,17(2):33-35
通过对优质食用鱼类—鳗鱼肌肉的氨基酸进行测定证实,鳗鱼较之其它鱼类是一种营养价值更高、滋味更鲜美的鱼类。并且,根据结果氨基酸组成比例,可为鳗鱼的人工饲养等方面的研究提供理论依据。 相似文献
224.
225.
Yu. A. Kuznetsov M. Ya. Antonovsky V. N. Biktashev E. A. Aponina 《Journal of mathematical biology》1994,32(3):219-232
A simple mathematical model of mono-species forest with two age classes which takes into account seed production and dispersal is presented in the paper. This reaction — diffusion type model is then reduced by means of an asymptotic procedure to a lower dimensional reaction — cross-diffusion model. The existence of standing and travelling wave front solutions corresponding to the forest boundary is shown for the later model. On the basis of the analysis, possible changes in forest boundary dynamics caused by antropogenic impacts are discussed. 相似文献
226.
A gene at 59 minutes on the Escherichia coli chromosome encodes a lipoprotein with unusual amino acid repeat sequences. 总被引:9,自引:5,他引:4
下载免费PDF全文
![点击此处可从《Journal of bacteriology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
We report a 1.432-kb DNA sequence at 59 min on the Escherichia coli chromosome that connects the published sequences of the pcm gene for the isoaspartyl protein methyltransferase and that of the katF or rpoS (katF/rpoS) gene for a sigma factor involved in stationary-phase gene expression. Analysis of the DNA sequence reveals an open reading frame potentially encoding a polypeptide of 379 amino acids. The polypeptide sequence includes a consensus bacterial lipidation sequence present at residues 23 to 26 (Leu-Ala-Gly-Cys), four octapeptide proline- and glutamine-rich repeats of consensus sequence QQPQIQPV, and four heptapeptide threonine- and serine-rich repeats of consensus sequence PTA(S,T)TTE. The deduced amino acid sequence, especially in the C-terminal region, is similar to that of the Haemophilus somnus LppB lipoprotein outer membrane antigen (40% overall sequence identity; 77% identity in last 95 residues). The LppB lipoprotein binds Congo red dye and has been proposed to be a virulence determinant in H. somnus. Utilizing a plasmid construct with the E. coli gene under the control of a phage T7 promoter, we demonstrate the lipidation of this gene product by the incorporation of [3H]palmitic acid into a 42-kDa polypeptide. We also show that treatment of E. coli cells with globomycin, an inhibitor of the lipoprotein signal peptidase, results in the accumulation of a 46-kDa precursor. We thus designate the protein NlpD (new lipoprotein D). E. coli cells overexpressing NlpD bind Congo red dye, suggesting a common function with the H. somnus LppB protein. Disruption of the chromosomal E. coli nlpD gene by insertional mutagenesis results in decreased stationary-phase survival after 7 days. 相似文献
227.
DcrA, a c-type heme-containing methyl-accepting protein from Desulfovibrio vulgaris Hildenborough, senses the oxygen concentration or redox potential of the environment. 总被引:4,自引:3,他引:1
下载免费PDF全文
![点击此处可从《Journal of bacteriology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The amino acid sequence of DcrA from Desulfovibrio vulgaris Hildenborough, a strictly anaerobic, sulfate-reducing bacterium, indicated homology with the methyl-accepting chemotaxis proteins from enteric bacteria (A. Dolla, R. Fu, M. J. Brumlik, and G. Voordouw, J. Bacteriol. 174:1726-1733, 1992). The homology is restricted to the cytoplasmic C-terminal signaling domain. The periplasmic N-terminal sensor domain was found to contain a unique sequence, CHHCH, corresponding to a consensus c-type heme binding site. A pretreated, DcrA-specific polyclonal antiserum, generated against DcrA protein overproduced in Escherichia coli, was used for immunoprecipitation of 35S-labeled DcrA from D. vulgaris and Desulfovibrio desulfuricans G200(pJRFR2), a transconjugant that overexpresses functional DcrA. Labeling of the latter with the heme precursor 5-amino-[4-14C]levulinic acid, followed by immunoprecipitation, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and fluorography, confirmed the presence of c-type heme, while labeling with L-[methyl-3H]methionine in the absence of protein synthesis confirmed that DcrA is a methyl-accepting protein. The base liability of the incorporated radioactivity indicated methyl ester formation like that occurring in the methyl-accepting chemotaxis proteins of enteric bacteria. L-[methyl-3H]methionine labeling of D. desulfuricans G200(pJRFR2) under different conditions indicated that methyl labeling of DcrA decreased upon addition of oxygen and increased upon subsequent addition of the reducing agent dithionite. These results indicate that DcrA may serve as a sensor of oxygen concentration and/or redox potential. 相似文献
228.
Dmitry I. Nurminsky Yury Ya. Shevelyov Sergei V. Nuzhdin Vladimir A. Gvozdev 《Chromosoma》1994,103(4):277-285
The 60 kb repeats located in the distal heterochromatin of the X chromosome of Drosophila melanogaster were cloned in overlapping cosmids. These regions, designated as SCLRs, comprised the following types of repeated elements Stellate genes, which are known to be involved in spermatogenesis; copia-like retrotransposons; LINE elements, including amplified Type rDNA insertions; and rDNA fragments. The following steps in SCLR formation were hypothesized: insertion of mobile elements into the rDNA and Stellate gene clusters: internal tandem duplication events; recombination between the rDNA cluster and Stellate tandem repeat; and amplification of the whole SCLR structure. There are about nine SCLR copies per haploid genome, but there is approximately a twofold variation in copy number between fly stocks. The SCLR copy number differences between closely related stocks are suggested to be the result of unequal sister chromatid exchange (USCE). The restricted variation in SCLR copy number between unrelated stocks and the absence of chromosomes free of SCLRs suggests that natural selection is active in copy number maintenance. 相似文献
229.
Map locations of mouse hepatitis virus temperature-sensitive mutants: confirmation of variable rates of recombination. 总被引:6,自引:6,他引:0
下载免费PDF全文
![点击此处可从《Journal of virology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Using standard genetic recombination techniques, studies in our laboratory suggest that recombination rates are very high and vary in different portions of the mouse hepatitis virus (MHV) genome. To determine the actual recombination frequencies in the MHV genome and localize the nucleotide boundaries of individual viral genes, we have sequenced temperature-sensitive and revertant viruses to identify the location of specific mutant alleles. Complementation group F RNA+ ts mutants (LA7, NC6, and NC16) each contained a unique mutation which was tightly linked to the ts phenotype and resulted in a conservative or nonconservative amino acid change in the MHV S glycoprotein gene. In agreement with previous recombination mapping studies, the mutation in LA7 and NC6 mapped within the S1 domain while NC16 mapped within the S2 domain. To determine the map coordinates of the MHV polymerase genes, several RNA- mutants and their revertants belonging to complementation groups C (NC3 and LA9) and E (LA18 and NC4) were also sequenced. Mutations were identified in each virus that were tightly linked to the ts phenotype and resulted in either a conservative or nonconservative amino acid change. The group C allele spanned the ORF 1a/ORF 1b junction, while the group E mutants mapped at the C terminus of ORF 1b about 20 to 22 kb from the 5' end of the genome. Mutation rates, calculated from the reversion frequencies of plaque-purified ts viruses requiring a single nucleotide alteration for reversion, approached 1.32 (+/- 0.89) x 10(-4) substitutions per nucleotide site per round of template copying. Detailed recombination mapping studies across known distances between these different ts alleles has confirmed that homologous recombination rates approached 25% and varied within different portions of the MHV genome. 相似文献
230.