全文获取类型
收费全文 | 140160篇 |
免费 | 4351篇 |
国内免费 | 5525篇 |
专业分类
150036篇 |
出版年
2024年 | 149篇 |
2023年 | 731篇 |
2022年 | 1645篇 |
2021年 | 2868篇 |
2020年 | 1901篇 |
2019年 | 2359篇 |
2018年 | 13625篇 |
2017年 | 11833篇 |
2016年 | 9275篇 |
2015年 | 3687篇 |
2014年 | 4008篇 |
2013年 | 4222篇 |
2012年 | 8679篇 |
2011年 | 16565篇 |
2010年 | 14284篇 |
2009年 | 10170篇 |
2008年 | 12152篇 |
2007年 | 13419篇 |
2006年 | 2272篇 |
2005年 | 2169篇 |
2004年 | 2395篇 |
2003年 | 2273篇 |
2002年 | 1826篇 |
2001年 | 1103篇 |
2000年 | 916篇 |
1999年 | 770篇 |
1998年 | 450篇 |
1997年 | 446篇 |
1996年 | 401篇 |
1995年 | 360篇 |
1994年 | 371篇 |
1993年 | 330篇 |
1992年 | 355篇 |
1991年 | 294篇 |
1990年 | 239篇 |
1989年 | 212篇 |
1988年 | 154篇 |
1987年 | 122篇 |
1986年 | 98篇 |
1985年 | 93篇 |
1984年 | 69篇 |
1983年 | 73篇 |
1982年 | 42篇 |
1980年 | 12篇 |
1979年 | 11篇 |
1972年 | 246篇 |
1971年 | 274篇 |
1965年 | 14篇 |
1962年 | 24篇 |
1944年 | 12篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
171.
Hung-Yen Hsieh Wen-Tseng Lo Don-Chung Liu Pei-Kai Hsu Wei-Cheng Su 《Environmental Biology of Fishes》2007,78(4):333-346
The aim of this study was to investigate the species composition and distribution of fish larvae in relation to hydrographic
conditions in the waters surrounding Taiwan Island (TI) in February 2003. In total, 242 kinds of fish larvae belonging to
127 genera and 75 families were recognized. Among these, 109 taxa were identified to the family or genus level, others to
the species level. The 12 predominant types, which constituted 71% of the total fish larvae, were Engraulis japonica, Scomber sp., Diaphus spp., Benthosema pterotum, Carangoides ferdau, Embolichthys mitsukurii, Maurolicus sp., unidentified Myctophidae, Gonostoma gracile, Trichiurus lepturus, unidentified Gobiidae, and Myctophum asperum. The distribution of fish larvae showed a clear association with water masses around TI, with higher abundances and lower
species richness northwest of TI where the China Coastal Current prevails, and lower abundances and higher species diversity
east of TI where the Kuroshio Current dominates. Cluster analysis distinguished three station groups and four species groups,
and the distribution patterns of fish larvae also corresponded to hydrographic conditions. The total abundances of fish larvae
and eight of the 12 predominant taxa showed significant and positive correlations with zooplankton abundance, which suggests
that food source might be a key factor determining the abundance and distribution of fish larvae during the winter. 相似文献
172.
A thermophilic bacterial strain GXN151 which could degrade Avicel efficiently was isolated and identified as Bacillus licheniformis. A genomic library of GXN151 was constructed and two novel endoglucanase genes designated cel9A and cel12A were isolated by screening the library on carboxylmethyl cellulase indicator plates. The analysis of amino acid sequences deduced from the genes indicated that Cel9A consisted of a catalytic domain belonging to glycosyl hydrolase family 9, a linker domain, and a carbohydrate binding module family 3 from N-terminal to C-terminal; Cel12A had only one catalytic domain belonging to glycosyl hydrolase family 12. The combinations of Cel9A and Cel12A produced by the recombinant E. coli exhibited synergistic action against substrates of carboxylmethyl cellulose as well as Avicel. 相似文献
173.
Hongdong Wang Chonghui Li Hongbin Xu Jianjun Hu Aiqun Zhang Sheng Ye Kesen Xu Jiahong Dong 《Cell biochemistry and biophysics》2014,68(1):55-65
Rat orthotopic liver transplantation (ROLT) remains a technically demanding procedure, especially regarding the reconstruction of the suprahepatic vena cava (SHVC). In this study, a new microsuture technique was developed for anastomosis of the SHVC, and a special single-groove cuff and blade-cut stent were introduced. With these modified techniques, we aimed to make a precise anastomosis of the SHVC and to provide optimal cuffs and stents for the reconstruction of the veins and bile ducts. According to different microsuture techniques for the SHVC and different types of cuffs and stents, three ROLT groups were created to compare the operation times and prognoses. Sham operations were performed as controls in the fourth group. The time expenditures with each step were compared among the transplantation groups. Biochemical parameters were tested at the end of a 1-month observation period. The short- and long-term survival rates of the transplantation groups were recorded and compared. Our new microsuture technique was faster than the conventional continuous suture technique for SHVC anastomosis (P < 0.05). The use of a single-groove cuff for reconstruction of the portal vein and the infrahepatic vena cava shortened the anastomotic time (P < 0.05). The use of blade-cut stents resulted in fewer biliary complications and better survival over the short and long terms (P < 0.05). Our new microsuture technique and the single-groove cuffs proved to be a precise method for venous reconstruction which shortened the anhepatic time and the anastomotic time significantly. The blade-cut stents apparently reduced the incidence of biliary complications. In summary, with this precise microsuture technique and delicate cuffs and stents, excellent long-term survival can be achieved easily and stably for ROLT. 相似文献
174.
We examined the relationship between body size of the riparian spider Nephila clavata and the contribution of allochthonous (aquatic insects) and autochthonous (terrestrial insects) sources to its diet using
stable isotope analysis. During the study period from July to September, the body size of the females increased remarkably
(about 60-fold) but that of males remained small. The biomass of both aquatic and terrestrial insects trapped on the spider
webs increased with spider size, with the biomass of the former ranging between 30 and 70% of that of the terrestrial insects.
The average relative contribution of aquatic insects to the diet of the spiders, calculated from δ13C values, was 40–50% in spiders in the early juvenile and juvenile stages, 35% in adult males and 4% in adult females. There
was a significant negative relationship between the relative contribution of aquatic insects and body size of the female spiders.
We conclude that aquatic insects might be an important seasonal dietary subsidy for small spiders and that these allochthonous
subsidies may facilitate the growth of riparian spiders, which may in turn enable the spiders to feed on larger prey. 相似文献
175.
Linda S. M. Ooi Wing-Shan Ho Karry L. K. Ngai Li Tian Paul K. S. Chan Samuel S. M. Sun Vincent E. C. Ooi 《Journal of biosciences》2010,35(1):95-103
A mannose-binding lectin (Narcissus tazetta lectin [NTL]) with potent antiviral activity was isolated and purified from the bulbs of the Chinese daffodil Narcissus tazetta var. chinensis, using ion exchange chromatography on diethylaminoethyl (DEAE)-cellulose, affinity chromatography on mannose-agarose and
fast protein liquid chromatography (FPLC)-gel filtration on Superose 12. The purified lectin was shown to have an apparent
molecular mass of 26 kDa by gel filtration and 13 kDa by SDS-PAGE, indicating that it is probably a dimer with two identical
subunits. The cDNA-derived amino acid sequence of NTL as determined by molecular cloning also reveals that NTL protein contains
a mature polypeptide consisting of 105 amino acids and a C-terminal peptide extension. Three-dimensional modelling study demonstrated
that the NTL primary polypeptide contains three subdomains, each with a conserved mannose-binding site. It shows a high homology
of about 60%–80% similarity with the existing monocot mannose-binding lectins. NTL could significantly inhibit plaque formation
by the human respiratory syncytial virus (RSV) with an IC50 of 2.30 μg/ml and exhibit strong antiviral properties against influenza A (H1N1, H3N2, H5N1) and influenza B viruses with
IC50 values ranging from 0.20 μg/ml to 1.33 μg/ml in a dose-dependent manner. It is worth noting that the modes of antiviral action
of NTL against RSV and influenza A virus are significantly different. NTL is effective in the inhibition of RSV during the
whole viral infection cycle, but the antiviral activity of NTL is mainly expressed at the early stage of the viral cycle of
influenza A (H1N1) virus. NTL with a high selective index (SI=CC50/IC50≥141) resulting from its potent antiviral activity and low cytotoxicity demonstrates a potential for biotechnological development
as an antiviral agent. 相似文献
176.
177.
178.
Nishanta Rajakaruna 《The Botanical review》2018,84(1):39-78
Plants adapted to special soil types are ideal for investigating evolutionary processes, including maintenance of intraspecific variation, adaptation, reproductive isolation, ecotypic differentiation, and the tempo and mode of speciation. Common garden and reciprocal transplant approaches show that both local adaptation and phenotypic plasticity contribute to edaphic (soil-related) specialization. Edaphic specialists evolve rapidly and repeatedly in some lineages, offering opportunities to investigate parallel evolution, a process less commonly documented in plants than in animals. Adaptations to soil features are often under the control of major genes and they frequently have direct or indirect effects on genes that contribute to reproductive isolation. Both reduced competitiveness and greater susceptibility to herbivory have been documented among some edaphic specialists when grown in ‘normal’ soils, suggesting that a high physiological cost of tolerance may result in strong divergent selection across soil boundaries. Interactions with microbes, herbivores, and pollinators influence soil specialization either by directly enhancing tolerance to extremes in soil conditions or by reducing gene flow between divergent populations. Climate change may further restrict the distribution of edaphic specialists due to increased competition from other taxa or, expand their ranges, if preadaptations to drought or other abiotic stressors render them more competitive under a novel climate. 相似文献
179.
Elke EM Brouwers Alwin DR Huitema Jos H Beijnen Jan HM Schellens 《BMC clinical pharmacology》2008,8(1):7
Background
The aim of this study was to evaluate long-term platinum retention in patients treated with cisplatin and oxaliplatin. 相似文献180.
Xuefang Wang Liuxiong Xu Yong Chen Guoping Zhu Siquan Tian Jiangfeng Zhu 《Aquatic Ecology》2012,46(3):343-352
Tuna purse seine fisheries target fish aggregated in schools, including free schools that are formed naturally based on fish biology and aggregations associated with natural and/or artificial drifting objects. Using data collected from skipjack tuna (Katsuwonus pelamis) fisheries, we evaluated differences in size structures between drifting-floating-object-associated schools and unassociated schools. We developed a generalized linear model to remove impacts of environmental variables on skipjack size composition. This study indicates that the drifting-floating-object-associated schools tended to have significantly wider size ranges than the unassociated schools. This suggests that unassociated schools were likely formed based on similarity in sizes among individuals within a school while drifting-floating-object-associated schools were probably composed of individuals of large size ranges and their formation was not based on the “size selection” rule. We concluded that the unassociated schools and the drifting-floating-object-associated schools were formed through different mechanisms, and drifting floating objects could aggregate unassociated schools of different size structures. Thus, a large scale of deployment of man-made floating objects might disrupt the spatial aggregation pattern of fish that otherwise tended to school based on their sizes in the absence of floating objects. 相似文献