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71.
72.
Jumping spiders (Salticidae) usually avoid ants, but some specieswithin this family single out ants as preferred prey, whileothers (especially the species in the genus Myrmarachne) areBatesian mimics of ants. Field records show that ant-eatingsalticids sometimes prey on Myrmarachne, suggesting that theunwanted attention of predators that specialize on the modelmay be an important, but poorly understood, cost of Batesianmimicry. By staging encounters in the laboratory between livingant-eating salticids and Myrmarachne, we determined that ant-eatingsalticids attack Myrmarachne. However, when Myrmarachne detectsa stalking ant-eating salticid early enough, it adopts a distinctivedisplay posture (legs almost fully extended, elevated 45°,and held out to the side 45°), and this usually deters thepredator. When Myrmarachne detects an ant-eating salticid beforestalking begins, Myrmarachne makes preemptive displays thatappear to inhibit the initiation of stalking. Using immobilelures made from dead Myrmarachne that were either in a displayposture or a nondisplay posture, we ascertained that specificallythe display posture of Myrmarachne deters the initiation ofstalking (ant-eating salticids stalked nondisplaying more oftenthan displaying lures). In another experiment, we ascertainedthat it is specifically the interjection of display posturethat deters stalking. When ant-eating salticids that had alreadybegun stalking experienced lures that switched from a nondisplayto a display posture, they stopped stalking. Although the unwantedattentions of its models' predators may be, for Myrmarachne,a hidden cost of Batesian mimicry, Myrmarachne appears to havean effective defense against these predators. 相似文献
73.
Patrick R. Sears Ximena M. Bustamante-Marin Henry Gong Matthew R. Markovetz Richard Superfine David B. Hill Lawrence E. Ostrowski 《Biophysical journal》2021,120(8):1387-1395
Impaired mucociliary clearance (MCC) is a key feature of many airway diseases, including asthma, bronchiectasis, chronic obstructive pulmonary disease, cystic fibrosis, and primary ciliary dyskinesia. To improve MCC and develop new treatments for these diseases requires a thorough understanding of how mucus concentration, mucus composition, and ciliary activity affect MCC, and how different therapeutics impact this process. Although differentiated cultures of human airway epithelial cells are useful for investigations of MCC, the extent of ciliary coordination in these cultures varies, and the mechanisms controlling ciliary orientation are not completely understood. By introducing a pattern of ridges and grooves into the underlying collagen substrate, we demonstrate for the first time, to our knowledge, that changes in the extracellular matrix can induce ciliary alignment. Remarkably, 90% of human airway epithelial cultures achieved continuous directional mucociliary transport (MCT) when grown on the patterned substrate. These cultures maintain transport for months, allowing carefully controlled investigations of MCC over a wide range of normal and pathological conditions. To characterize the system, we measured the transport of bovine submaxillary gland mucin (BSM) under several conditions. Transport of 5% BSM was significantly reduced compared with that of 2% BSM, and treatment of 5% BSM with the reducing agent tris(2-carboxyethyl)phosphine (TCEP) reduced viscosity and increased the rate of MCT by approximately twofold. Addition of a small amount of high-molecular-weight DNA increased mucus viscosity and reduced MCT by ~75%, demonstrating that the composition of mucus, as well as the concentration, can have significant effects on MCT. Our results demonstrate that a simple patterning of the collagen substrate results in highly coordinated ciliated cultures that develop directional MCT, and can be used to investigate the mechanisms controlling the regulation of ciliary orientation. Furthermore, the results demonstrate that this method provides an improved system for studying the effects of mucus composition and therapeutic agents on MCC. 相似文献
74.
75.
Leukocyte chemotactic activity of cyclophilin. 总被引:14,自引:0,他引:14
Q Xu M C Leiva S A Fischkoff R E Handschumacher C R Lyttle 《The Journal of biological chemistry》1992,267(17):11968-11971
During the purification of eosinophil chemotactic factors synthesized by the uterus in response to estrogen we isolated a protein having an N-terminal (15 amino acids) sequence identical to that of rat cyclophilin. Our data demonstrate that cyclophilin, a cytosolic protein isolated from bovine thymocytes, which specifically binds the immunosuppressive drug cyclosporin A, as well as recombinant human cyclophilin, displays eosinophil chemotactic activity. In addition to its chemotactic activity, cyclophilin stimulated the release of peroxidase activity from eosinophils. Maximal chemotactic activity of cyclophilin was achieved at a concentration of approximately 10 nM. At similar concentrations cyclophilin was also able to stimulate the migration of neutrophils. This chemotactic activity could be prevented by the addition of cyclosporin A, but not by a nonimmunosuppressive analog (1-fur-furyl-cyclosporin A) at similar concentrations. This chemotactic activity may represent an additional mechanism by which immunosuppressive drugs function to prevent tissue rejection. 相似文献
76.
Ontogeny of the production of an estrogen-regulated eosinophil chemotactic factor in the rat uterus.
The ontogeny of an estrogen-induced eosinophil chemotactic activity in the uterus after estrogen administration and its regulation by progesterone was studied in neonatal rats at 3, 7, 10, and 13 days of age. These results were compared with the increase in peroxidase activity and the secretion of complement component C3 by the uterus. No chemotactic activity was observed in animals younger than 14 days, and after this age the estrogen regulation of the response was also prevented by the co-administration of progesterone. The secretion of complement C3 appeared earlier, at 10 days of age. It was also observed that the uterus of 3-7-day-old rats responded to estrogen by increasing the secretion of two proteins of 36 and 110 kDa. No further regulation or synthesis was observed with increasing age for these two proteins. 相似文献
77.
Hernan Lara Ximena Galleguillos Jorge Arrau Jorge Belmar 《Neurochemistry international》1985,7(4):667-674
Norepinephrine and dopamine-β-hydroxylase, used as noradrenergic vesicle markers, were found to be decreased in the rat vas deferens 10 days after castration. Five days of testosterone administration to castrated animals increased the enzyme activity over that of controls but did not modify norepinephrine content. In tissue fractions obtained by differential centrifugation, the highest activities of the noradrenergic markers appeared in the vesicular fraction of controls and in the soluble fraction of castrated animals. Testosterone reversed the effect of castration: it increased dopamine-β-hydroxylase activity in the vesicular and soluble fractions, while norepinephrine increased only in the vesicular fraction. Results obtained after continuous sucrose gradient centrifugation of vesicular fractions suggested that these changes principally affected the number of light noradrenergic vesicles while testosterone increased the number of vesicles reduced by castration. Hormonal manipulations also modified some functional properties of nerve endings: norepinephrine depletion after transmural stimulation in the presence of tetraethylammonium, as well as the release of the neurotransmitter, were decreased after castration. These effects were reversed by testosterone. The results suggest a modulatory effect of testosterone on the norepinephrine storage system and on the functional properties of the adrenergic innervation of vas deferens. 相似文献
78.
Fredric V. Vencl Kristina Ottens Marjorie M. Dixon Sarah Candler Ximena E. Bernal Catalina Estrada Rachel A. Page 《Biotropica》2016,48(5):645-655
Although famous for photic courtship displays, fireflies (Coleoptera: Lampyridae) are also notable for emitting strong odors when molested. The identity of volatile emissions and their possible role, along with photic signals, as aposematic warnings of unpalatability have been little explored, especially in tropical species. Pursuant to the observation that the widespread Neotropical fireflies, Photuris trivittata and Bicellonycha amoena, emit pungent odors, glows, and flashes when handled, we investigated their cuticular and headspace chemistry. Gas chromatography–mass spectrometry analyses revealed that both fireflies have species‐specific cuticular hydrocarbon profiles. Photuris trivittata headspace was dominated by 2‐methoxy‐3‐(1‐methylpropyl) pyrazine (hereafter, pyrazine), on the order of 1.59 ng/individual and a suite of sesquiterpenes, while B. amoena emitted 3‐methoxy‐2‐butenoic acid methyl ester and a few ketones. This is the first report of such compounds in fireflies. We investigated the role of pyrazine in P. trivittata's interactions with potential predators: sympatric ants, toads, and bats. Solvent‐washed P. trivittata painted with pyrazine incurred lower ant predation than did their solvent‐washed counterparts. Pyrazine significantly repelled ants at baits in concentrations as low as 9.8 × 10?4 ng/μl. The toad, Rhinella marina, readily accepted intact fireflies, pyrazine‐coated and uncoated mealworms. Both Myotis nigricans and Molossus molossus bats rejected fireflies, but accepted both pyrazine‐coated and uncoated mealworms. While pyrazine repels ants, its role as an aposematic signal warning other potential predators of firefly distastefulness requires further investigation. Our results underscore the idea that multiple enemies exert conflicting selection on firefly defenses. 相似文献
79.
Metabolomics‐guided insights on bariatric surgery versus behavioral interventions for weight loss
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80.
Johany Pe?ailillo Gabriela Olivares Ximena Moncada Claudia Payacán Chi-Shan Chang Kuo-Fang Chung Peter J. Matthews Andrea Seelenfreund Daniela Seelenfreund 《PloS one》2016,11(8)
BackgroundPaper mulberry (Broussonetia papyrifera (L.) L''Hér. ex Vent) is a dioecious tree native to East Asia and mainland Southeast-Asia, introduced prehistorically to Polynesia as a source of bark fiber by Austronesian-speaking voyagers. In Oceania, trees are coppiced and harvested for production of bark-cloth, so flowering is generally unknown. A survey of botanical records of paper mulberry revealed a distributional disjunction: the tree is apparently absent in Borneo and the Philippines. A subsequent study of chloroplast haplotypes linked paper mulberry of Remote Oceania directly to a population in southern Taiwan, distinct from known populations in mainland Southeast-Asia.MethodologyWe describe the optimization and use of a DNA marker designed to identify sex in paper mulberry. We used this marker to determine the sex distribution in selected localities across Asia, Near and Remote Oceania. We also characterized all samples using the ribosomal internal transcribed spacer sequence (ITS) in order to relate results to a previous survey of ITS diversity.ResultsIn Near and Remote Oceania, contemporary paper mulberry plants are all female with the exception of Hawaii, where plants of both sexes are found. In its natural range in Asia, male and female plants are found, as expected. Male plants in Hawaii display an East Asian ITS genotype, consistent with modern introduction, while females in Remote Oceania share a distinctive variant.ConclusionsMost paper mulberry plants now present in the Pacific appear to be descended from female clones introduced prehistorically. In Hawaii, the presence of male and female plants is thought to reflect a dual origin, one a prehistoric female introduction and the other a modern male introduction by Japanese/Chinese immigrants. If only female clones were dispersed from a source-region in Taiwan, this may explain the absence of botanical records and breeding populations in the Philippines and Borneo, and Remote Oceania. 相似文献