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111.
Phylogeny and historical biogeography of the cocosoid palms (Arecaceae,Arecoideae, Cocoseae) inferred from sequences of six WRKY gene family loci
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Alan W. Meerow Larry Noblick Dayana E. Salas‐Leiva Vanessa Sanchez Javier Francisco‐Ortega Brett Jestrow Kyoko Nakamura 《Cladistics : the international journal of the Willi Hennig Society》2015,31(5):509-534
Arecaceae tribe Cocoseae is the most economically important tribe of palms, including both coconut and African oil palm. It is mostly represented in the Neotropics, with one and two genera endemic to South Africa and Madagascar, respectively. Using primers for six single copy WRKY gene family loci, we amplified DNA from 96 samples representing all genera of the palm tribe Cocoseae as well as outgroup tribes Reinhardtieae and Roystoneae. We compared parsimony (MP), maximum likelihood (ML), and Bayesian (B) analysis of the supermatrix with three species‐tree estimation approaches. Subtribe Elaeidinae is sister to the Bactridinae in all analyses. Within subtribe Attaleinae, Lytocaryum, previously nested in Syagrus, is now positioned by MP and ML as sister to the former, with high support; B maintains Lytocaryum embedded within Syagrus. Both MP and ML resolve Cocos as sister to Syagrus; B positions Cocos as sister to Attalea. Bactridineae is composed of two sister clades, Bactris and Desmoncus in one, for which there is morphological support, and a second comprising Acrocomia, Astrocaryum, and Aiphanes. Two B and one ML gene tree‐species estimation approaches are incongruent with the supermatrix in a few critical intergeneric clades, but resolve the same infrageneric relationships. The biogeographic history of the Cocoseae is dominated by dispersal events. The tribe originated in the late Cretaceous in South America. Evaluated together, the supermatrix and species tree analyses presented in this paper provide the most accurate picture of the evolutionary history of the tribe to date, with more congruence than incongruence among the various methodologies. 相似文献
112.
Olivier le Guen Rumen Iliev Ximena Lois Scott Atran Douglas L. Medin 《The journal of the Royal Anthropological Institute》2013,19(4):771-794
This study reports ethnographic and experimental analyses of inter‐generational changes in native Itza' Maya and immigrant Ladino populations of Guatemala's Petén rainforest concerning understanding of ecological relationships between plants, animals, and humans, and the perceived role of forest spirits in sustaining these relationships. We find dramatic changes in understanding ecological relationships and the perceived role of forest spirits. Itza' Maya conceptions of forest spirits (arux) are now more often confounded with Ladino spirits (duendes), with Itza' spirits no longer reliably serving as forest guardians. These changes correlate with a shift in personal values regarding the forest, away from concern with ecologically central trees and towards monetary incentives. More generally, we describe how economic, demographic, and social changes relate to the loss of a system of beliefs and behaviours that once promoted sustainable agro‐forestry practices. These changes coincide with open access to common pool resources. 相似文献
113.
Abrevaya XC Sacco N Mauas PJ Cortón E 《Extremophiles : life under extreme conditions》2011,15(6):633-642
In this work, two archaea microorganisms (Haloferax volcanii and Natrialba magadii) used as biocatalyst at a microbial fuel cell (MFC) anode were evaluated. Both archaea are able to grow at high salt concentrations.
By increasing the media conductivity, the internal resistance was diminished, improving the MFC’s performance. Without any
added redox mediator, maximum power (P
max) and current at P
max were 11.87/4.57/0.12 μW cm−2 and 49.67/22.03/0.59 μA cm−2 for H. volcanii, N. magadii and E. coli, respectively. When neutral red was used as the redox mediator, P
max was 50.98 and 5.39 μW cm−2 for H. volcanii and N. magadii, respectively. In this paper, an archaea MFC is described and compared with other MFC systems; the high salt concentration
assayed here, comparable with that used in Pt-catalyzed alkaline hydrogen fuel cells, will open new options when MFC scaling
up is the objective necessary for practical applications. 相似文献
114.
Elimination of neglected diseases in latin america and the Caribbean: a mapping of selected diseases
Schneider MC Aguilera XP Barbosa da Silva Junior J Ault SK Najera P Martinez J Requejo R Nicholls RS Yadon Z Silva JC Leanes LF Periago MR 《PLoS neglected tropical diseases》2011,5(2):e964
In Latin America and the Caribbean, around 195 million people live in poverty, a situation that increases the burden of some infectious diseases. Neglected diseases, in particular, are often restricted to poor, marginalized sections of the population. Tools exist to combat these diseases, making it imperative to work towards their elimination. In 2009, the Pan American Health Organization (PAHO) received a mandate to support the countries in the Region in eliminating neglected diseases and other poverty-related infections. The objective of this study is to analyze the presence of selected diseases using geo-processing techniques. Five diseases with information available at the first sub-national level (states) were mapped, showing the presence of the disease ("hotspots") and overlap of diseases ("major hotspots"). In the 45 countries/territories (approximately 570 states) of the Region, there is: lymphatic filariasis in four countries (29 states), onchocerciasis in six countries (25 states), schistosomiasis in four countries (39 states), trachoma in three countries (29 states), and human rabies transmitted by dogs in ten countries (20 states). Of the 108 states with one or more of the selected diseases, 36 states present the diseases in overlapping areas ("major hotspots"). Additional information about soil-transmitted helminths was included. The analysis suggests a majority of the selected diseases are not widespread and can be considered part of an unfinished agenda with elimination as a goal. Integrated plans and a comprehensive approach, ensuring access to existing diagnostic and treatment methods, and establishing a multi-sectoral agenda that addresses social determinants, including access to adequate water and sanitation, are required. Future studies can include additional diseases, socio-economic and environmental variables. 相似文献
115.
Horst Lüddecke Adolfo Amézquita Ximena Bernal Francisco Guzmán 《Studies on Neotropical Fauna and Environment》2013,48(3):185-194
Vocal activity was studied in a Colombian highland-frog community, consisting of five frog species belonging to three families with three different reproductive modes, which reproduced simultaneously in the area. We encountered one diurnal and four nocturnal species. Each species had a distinct call structure, and the 24-hour patterns of calling activity differed significantly between all but two species. Interspecific differences in dominant call frequencies corresponded inversely to differences in male snout-vent length. Only one species called in ponds, whereas all other species used terrestrial sites. Among the terrestrial callers there seemed to be interspecific differences in plant use as calling sites. Our results indicate that despite the low number of species, interspecific vocal partitioning is pronounced, and probably important for reliable vocal communication. 相似文献
116.
Experiments on monomeric proteins have shown that macromolecular crowding can stabilize toward heat perturbation and also modulate native-state structure. To assess the effects of macromolecular crowding on unfolding of an oligomeric protein, we here tested the effects of the synthetic crowding agent Ficoll 70 on human cpn10 (GroES in E. coli), a heptameric protein consisting of seven identical β-barrel subunits assembling into a ring. Using far-UV circular dichroism (CD), tyrosine fluorescence, nuclear magnetic resonance (NMR), and cross-linking experiments, we investigated thermal and chemical stability, as well as the heptamer-monomer dissociation constant, without and with crowding agent. We find that crowding shifts the heptamer-monomer equilibrium constant in the direction of the heptamer. The cpn10 heptamer is both thermally and thermodynamically stabilized in 300 mg/mL Ficoll 70 as compared to regular buffer conditions. Kinetic unfolding experiments show that the increased stability in crowded conditions, in part, is explained by slower unfolding rates. A thermodynamic cycle reveals that in presence of 300 mg/mL Ficoll the thermodynamic stability of each cpn10 monomer increases by over 30%, whereas the interfaces are stabilized by less than 10%. We also introduce a new approach to analyze the spectroscopic data that makes use of multiple wavelengths: this provides robust error estimates of thermodynamic parameters. 相似文献
117.
118.
Arredondo M Fuentes M Jorquera D Candia V Carrasco E Leiva E Mujica V Hertrampf E Pérez F 《Biological trace element research》2011,143(2):625-636
To assess the relationship between the length of (GT)
n
repeats in HO-1 gene promoter and heme oxygenase (HO) enzymatic activity in mononuclear cells with iron (Fe) stores in type
2 diabetic mellitus (DM2) patients and metabolic syndrome (MS) subjects, we studied 163 patients with DM2, 185 with MS, and
120 controls subjects. We evaluated iron status (hemoglobin and serum Fe, ferritin, and transferrin receptor), and we determined
the length of (GT)
n
repeats in HO-1 gene promoter by capillary electrophoresis and HO enzymatic activity in mononuclear cells and assessed the
relationship between these results and Fe stores. Only 1/163, 6/185, and 7/120 had iron deficiency anemia in DM2 patients,
MS subjects, and controls, respectively. No iron overload (ferritin >200 μg/L) was detected in all the subjects studied. DM2
patients had higher iron deposits, total body iron, and heme oxygenase activity (a suggestion of high oxidative stress condition)
than MS subjects and controls. In DM2, we found a positive association between serum iron and HO activity. There were no difference
in allelic frequency between the three groups; however, among DM2 and MS patients, the frequency of short/medium (SM) genotype
of (GT)
n
repetition was increased and medium/medium (MM) genotype of (GT)
n
repetition was lower than controls. These results imply that DM2 patients and individuals with MS carrying SM repeats might
have higher susceptibility to develop diabetes consequences. This increased susceptibility could be Fe-mediated oxidative
stress. 相似文献
119.
Yáñez AJ Bustamante X Bertinat R Werner E Rauch MC Concha II Reyes JG Slebe JC 《Journal of cellular physiology》2007,212(3):807-816
A substrate cycle composed of phosphofructo 1-kinase I (PFK) and fructose 1,6 bisphosphatase I (FBPase) has been proposed in rat spermatids. This substrate cycle can explain the ability of glucose to induce a decrease in intracellular ATP, a phenomenon that was related to regulation of [Ca(2+)]i in these cells. In spite of the importance of this metabolic cycle, the expression and activities of the enzymes that compose such cycle have not been systematically studied in spermatogenic cells. Here, we show that PFK and FBPase activities were present in pachytene spermatocytes and round spermatids extracts. Expression of PFK at the mRNA and protein levels showed a relatively similar expression in spermatogenic cells, but a stronger expression in Sertoli cells. Instead, expression of FBPase at the mRNA and protein levels was stronger in round and elongating spermatids as compared to other spermatogenic cells. A similar pattern was observed when evidencing FBPase activity by a NADPH-nitroblue tetrazolium-linked cytochemical assay in isolated pachytene spermatocytes and round spermatids. Rat spermatids also showed the ability to convert lactate to fructose- and glucose-6-P, indicating that both glycolytic and gluconeogenic fluxes are present in these cells. Our results indicate that a coordinated expression of key substrate cycle enzymes, at the level of PFK/FBPase, appear in the last stages of spermatogenic cell differentiation, suggesting that the co-regulation of these enzymes are required for the ability of these cells to respond to glucose and induce metabolic and Ca(2+) signals that can be important for sperm development and function. 相似文献
120.
Ramirez-Espain X Ruiz L Martin-Malpartida P Oschkinat H Macias MJ 《Journal of molecular biology》2007,373(5):1255-1268
Formin homology 1 (FH1), is a long proline-rich region of formins, shown to bind to five WW containing proteins named formin binding proteins (FBPs). FH1 has several potential binding regions but only the PPLPx motif and its interaction with FBP11WW1 has been characterized structurally. To detect whether additional motifs exist in FH1, we synthesized five peptides and investigated their interaction with FBP28WW2, FBP11WW1 and FBP11WW2 domains. Peptides of sequence PTPPPLPP (positive control), PPPLIPPPP and PPLIPPPP (new motifs) interact with the domains with micromolar affinity. We observed that FBP28WW2 and FBP11WW2 behave differently from FBP11WW1 in terms of motif selection and affinity, since they prefer a doubly interrupted proline stretch of sequence PPLIPP. We determined the NMR structure of three complexes involving the FBP28WW2 domain and the three ligands. Depending on the peptide under study, the domain interacts with two proline residues accommodated in either the XP or the XP2 groove. This difference represents a one-turn displacement of the domain along the ligand sequence. To understand what drives this behavior, we performed further structural studies with the FBP11WW1 and a mutant of FBP28WW2 mimicking the XP2 groove of FBP11WW1. Our observations suggest that the nature of the XP2 groove and the balance of flexibility/rigidity around loop 1 of the domain contribute to the selection of the final ligand positioning in fully independent domains. Additionally, we analyzed the binding of a double WW domain region, FBP11WW1-2, to a long stretch of FH1 using fluorescence spectroscopy and NMR titrations. With this we show that the presence of two consecutive WW domains may also influence the selection of the binding mode, particularly if both domains can interact with consecutive motifs in the ligand. Our results represent the first observation of protein-ligand recognition where a pair of WW and two consecutive motifs in a ligand participate simultaneously. 相似文献