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951.
The effect of cyclic anaerobic–aerobic conditions on the biodegradative capability of the mixed microbial culture for the
azo dye Remazol Brilliant Violet 5R (RBV-5R) was investigated in the sequencing batch reactor (SBR) fed with a synthetic textile
wastewater. The SBR had a 12-h cycle time with anaerobic–aerobic periods of 3/9, 6/6 and 9/3 h. General SBR performance was
assessed by measurement of catabolic enzymes (catechol 2,3-dioxygenase, azo reductase), chemical oxygen demand (COD), color
and amount of aromatic amines. In this study, under steady-state conditions, the anaerobic period of the cyclic SBR was found
to allow the reductive decolorization of azo dye. Longer anaerobic periods resulted in higher color removal efficiencies,
approximately 71% for the 3-h, 87% for 6-h and 92% for the 9-h duration. Total COD removal efficiencies were over 84% under
each of the cyclic conditions and increased as the length of the anaerobic period was increased; however, the highest color
removal rate was attained for the cycle with the shortest anaerobic period of 3 h. During the decolorization of RBV-5R, two
sulfonated aromatic amines (benzene based and naphthalene based) were formed. Additionally, anaerobic azo reductase enzyme
was found to be positively affected with the increasing duration of the anaerobic period; however; it was vice versa for the
aerobic catechol 2,3-dioxygenase (C23DO) enzyme. 相似文献
952.
Takehiro Ohta Perumandla Nagaraju Jin-Gang Liu Takashi Ogura Yoshinori Naruta 《Journal of biological inorganic chemistry》2016,21(5-6):745-755
Oxygen reduction reaction (ORR) catalyzed by a bio-inspired iron porphyrin bearing a hanging carboxylic acid group over the porphyrin ring, and a tethered axial imidazole ligand was studied by DFT calculations. BP86 free energy calculations of the redox potentials and pK a’s of reaction components involved in the proton coupled electron transfer (PCET) reactions of the ferric-hydroxo and -superoxo complexes were performed based on Born–Haber thermodynamic cycle in conjunction with a continuum solvation model. The comparison was made with iron porphyrins that lack either in the hanging acid group or axial ligand, suggesting that H-bond interaction between the carboxylic acid and iron-bound hydroxo, aquo, superoxo, and peroxo ligands (de)stabilizes the Fe–O bonding, resulting in the increase in the reduction potential of the ferric complexes. The axial ligand interaction with the imidazole raises the affinity of the iron-bound superoxo and peroxo ligands for proton. In addition, a low-spin end-on ferric-hydroperoxo intermediate, a key precursor for O–O cleavage, can be stabilized in the presence of axial ligation. Thus, selective and efficient ORR of iron porphyrin can be achieved with the aid of the secondary coordination sphere and axial ligand interactions. 相似文献
953.
Liting Qin Xiaole Qi Honglei Gao Yulong Gao Zhigao Bu Xiaomei Wang 《Journal of microbiology (Seoul, Korea)》2009,47(3):344-350
Infectious bursal disease virus (IBDV), belonging to Avibirnavirus genus in the Birnaviridae family, consists of two segments of double-strand RNA. There are two distinct serotypes of IBDV, the pathogenic serotype
I and the non-pathogenic serotype II. Comparison of the deduced amino acid sequences of a panel of VP5 genes retrieved from
GenBank revealed a high identity among strains within the serotype I or serotype II group but a low identity between strains
across two serotypes. In this study, we rescued two mosaic viruses, rGtGxVP5 and rGt2382VP5 by exchanging the VP5 gene of
a cell culture-adapted serotype I Gt strain with its counterpart of the very virulent IBDV Gx strain, or a non-pathogenic
23/82 strain of the serotype II. In comparison to the parental strain rGt virus, the rGtGxVP5 showed the similar viral replication,
cytotoxicity and the ability of inducing apoptosis; however, the other mosaic virus rGt2382VP5 had a lower titer and a reduced
cytotoxicity. Although exchange of VP5 within serotype I group did not alter the viral replication and cytotoxicity of Gt
strain, exchange of VP5 in the serotype I with that of a serotype II reduced the viral replication and cytotoxicity on chicken
embryo fibroblast (CEF) cells. Therefore, the VP5 of serotype II may be one of the factors responsible for the distinct pathogenic
features of two serotypes. 相似文献
954.
Cloning, sequencing, and expression of the gene encoding extracellular alpha-amylase from Pyrococcus furiosus and biochemical characterization of the recombinant enzyme. 总被引:2,自引:1,他引:2 下载免费PDF全文
The gene encoding the hyperthermophilic extracellular alpha-amylase from Pyrococcus furiosus was cloned by activity screening in Escherichia coli. The gene encoded a single 460-residue polypeptide chain. The polypeptide contained a 26-residue signal peptide, indicating that this Pyrococcus alpha-amylase was an extracellular enzyme. Unlike the P. furiosus intracellular alpha-amylase, this extracellular enzyme showed 45 to 56% similarity and 20 to 35% identity to other amylolytic enzymes of the alpha-amylase family and contained the four consensus regions characteristic of that enzyme family. The recombinant protein was a homodimer with a molecular weight of 100,000, as estimated by gel filtration. Both the dimer and monomer retained starch-degrading activity after extensive denaturation and migration on sodium dodecyl sulfate-polyacrylamide gels. The P. furiosus alpha-amylase was a liquefying enzyme with a specific activity of 3,900 U mg-1 at 98 degrees C. It was optimally active at 100 degrees C and pH 5.5 to 6.0 and did not require Ca2+ for activity or thermostability. With a half-life of 13 h at 98 degrees C, the P. furiosus enzyme was significantly more thermostable than the commercially available Bacillus licheniformis alpha-amylase (Taka-therm). 相似文献
955.
B. Akbar John B. R. Nelson Hassan I. Sheikh S. G. Cheung Yusli Wardiatno Bisnu Prasad Dash Keiji Tsuchiya Yumiko Iwasaki Siddhartha Pati 《Biodiversity and Conservation》2018,27(14):3573-3598
Horseshoe crabs are the only extant xiphosurans and are believed to be morphologically unchanged for more than 200 million years. Of the four extant species namely, Limulus polyphemus, Tachypleus tridentatus, Tapinauchenius gigas and Carcinoscorpius rotundicauda, the latter three are found in Asian waters. Recent evidences showed that Asian horseshoe crabs are facing serious threats such as degradation of their spawning grounds and habitat, environmental pollution, overexploitation as a culinary delicacy and biomedical bleeding practices. Baseline data on the distribution and existing population of the wild horseshoe crabs remain poorly known in several Asian regions. Several studies have clearly revealed that pressure due to over-fishing of wild stock has increased tremendously in the last decade. Due to an increase in demand for Tachypleus Amebocyte Lysate (TAL) analogous to Limulus Amebocyte Lysate (LAL) in the United States, there is an urgent need to comprehensively address their fishing and conservation measures in the Asian region. This review addresses the overall studies on three species of Asian horseshoe crabs in relation to their fishing practices, local exploitation of their wild stock either for human consumption (or) by biomedical industries. The authors have structured the discussion on an international scale to address the existing problems in fishing and conservation of horseshoe crabs. Since no specific regulatory force or legislative protection act or a policy to preserve their natural stock are available to this date, this paper strongly recommends representative countries to include horseshoe crabs under their wildlife protection act to avoid further unsustainable exploitation of their wild populations. 相似文献
956.
Daoyong Zhang Xiangliang Pan Guijin Mu Jianlong Wang 《Journal of applied phycology》2010,22(4):479-488
It has been demonstrated that antimony (Sb) at concentrations ranging from 1.0 to 10.0 mg L−1 inhibits O2 evolution. Deeper insight into the influence of Sb on PSII was obtained with measurements of in vivo chlorophyll fluorescence.
The donor and the acceptor sides of PSII were shown to be the target of Sb. Sb treatment induces inhibition of electron transport
from QA− to QB/QB− and accumulation of P680+. S2(QAQB)− charge recombination and oxidation by PQ9 molecules became more important in QA− reoxidation as the electron transfer in PSII was inhibited. Sb exposure caused a steady increase in the proportion of PSIIX and PSIIβ. These changes resulted in increased fluxes of dissipated energy and decreased index of photosynthesis performance, of maximum
quantum yield, and of the overall photosynthetic driving force of PSII. 相似文献
957.
Puligundla P Smogrovicova D Obulam VS Ko S 《Journal of industrial microbiology & biotechnology》2011,38(9):1133-1144
There have been numerous developments in ethanol fermentation technology since the beginning of the new millennium as ethanol
has become an immediate viable alternative to fast-depleting crude reserves as well as increasing concerns over environmental
pollution. Nowadays, although most research efforts are focused on the conversion of cheap cellulosic substrates to ethanol,
methods that are cost-competitive with gasoline production are still lacking. At the same time, the ethanol industry has engaged
in implementing potential energy-saving, productivity and efficiency-maximizing technologies in existing production methods
to become more viable. Very high gravity (VHG) fermentation is an emerging, versatile one among such technologies offering
great savings in process water and energy requirements through fermentation of higher concentrations of sugar substrate and,
therefore, increased final ethanol concentration in the medium. The technology also allows increased fermentation efficiency,
without major alterations to existing facilities, by efficient utilization of fermentor space and elimination of known losses.
This comprehensive research update on VHG technology is presented in two main sections, namely VHG brewing, wherein the effects
of nutrients supplementation, yeast pitching rate, flavour compound synthesis and foam stability under increased wort gravities
are discussed; and VHG bioethanol fermentation studies. In the latter section, aspects related to the role of osmoprotectants
and nutrients in yeast stress reduction, substrates utilized/tested so far, including saccharide (glucose, sucrose, molasses,
etc.) and starchy materials (wheat, corn, barley, oats, etc.), and mash viscosity issues in VHG bioethanol production are
detailed. Thereafter, topics common to both areas such as process optimization studies, mutants and gene level studies, immobilized
yeast applications, temperature effect, reserve carbohydrates profile in yeast, and economic aspects are discussed and future
prospects are summarized. 相似文献
958.
两种不同根系类型湿地植物的根系生长 总被引:19,自引:2,他引:19
实验设计了一个水培系统,利用生活污水培养,对4种“须根型”植物美人蕉、风车草、象草和香根草和4种根茎型植物菖蒲、水鬼蕉、芦苇和水烛的根系生长进行比较研究。该系统由用于盛污水的塑料桶(顶部直径36.5cm,底部直径30.Ocm,高34.5cm)和用于固定植物于水面的泡沫板构成。每桶种植1株植物,每种种5株。水培至10周时,须根型植物的平均根数达到1349条/株,而根茎型植物的平均根数只有549条/株。实验结束(水培第21周)时,须根型植物的平均根生物量为11.3g/株,根茎型植物的平均根生物量为7.4g/株。须根型植物根系中,d〈1mm的细根生物量占根系总生物量的51.9%,而根茎型植物d〈1mm的细根的生物量只占25.1%。根茎型植物的根生物量与地上生物量的比值为0.2,显著高于须根型湿地植物(0.1)。须根型湿地植物的根系表面积(6933cm^2/株)极显著地高于根茎型湿地植物(1897cm^2/株)。根茎型湿地植物根的平均寿命(46.6d)较须根型湿地植物根的平均寿命(34.8d)长。美人蕉的平均根数达1871条/株,根表面积达到22832cm^2/株,远较其他种高。 相似文献
959.
Diversity and dynamics of the bacterial community involved in pig manure biodegradation in a microbial fermentation bed system 总被引:8,自引:0,他引:8
Qianqian Chen Bo Liu Jieping Wang Jianmei Che Guohong Liu Xiong Guan 《Annals of microbiology》2017,67(7):491-500
Overproduction of livestock manures with unpleasant odors causes significant environmental problems. The microbial fermentation bed (MFB) system is considered an effective approach to recycling utilization of agricultural byproducts and pig manure (PM). To gain a better understanding of bacterial communities present during the degradation of PM in MFB, the PM bacterial community was evaluated at different fermentation stages using 16S rRNA high throughput sequencing technology. The heatmap plot clustered five samples into short-term fermentation stage of 0–10 days and long-term fermentation stage of 15–20 days. The most abundant OTUs at the phylum level were Firmicutes, Actinobacteria and Proteobacteria in the long-term fermentation stage of PM, whereas Firmicutes, Bacteroidetes, and Proteobacteria predominated in the short-term fermentation stage of PM. At the genus level, organic degradation strains, such as Corynebacterium, Bacillus, Virgibacillus, Pseudomonas, Actinobacteria, Lactobacillus, Pediococcus were the predominate genera at the long-term fermentation stage, but were found only rarely in the short-term fermentation stage. C/N ratios increased and the concentration of the unpleasant odor substance 3-hydroxy-5-methylisoxazole (3-MI) decreased with prolonged period of fermentation. Redundancy analysis (RDA) demonstrated that the relative abundance of Firmicutes, Actinobacteria, Acidobacteria and Proteobacteria had a close relationship with degradation of 3-MI and increasing C/N ratio. These results provide valuable additional information about bacterial community composition during PM biodegradation in animal husbandry. 相似文献
960.
Aloke K. Bera Nancy W. Y. Ho Aftab Khan Miroslav Sedlak 《Journal of industrial microbiology & biotechnology》2011,38(5):617-626
Robust microorganisms are necessary for economical bioethanol production. However, such organisms must be able to effectively
ferment both hexose and pentose sugars present in lignocellulosic hydrolysate to ethanol. Wild type Saccharomyces cerevisiae can rapidly ferment hexose, but cannot ferment pentose sugars. Considerable efforts were made to genetically engineer S. cerevisiae to ferment xylose. Our genetically engineered S cerevisiae yeast, 424A(LNH-ST), expresses NADPH/NADH xylose reductase (XR) that prefer NADPH and NAD+-dependent xylitol dehydrogenase (XD) from Pichia stipitis, and overexpresses endogenous xylulokinase (XK). This strain is able to ferment glucose and xylose, as well as other hexose
sugars, to ethanol. However, the preference for different cofactors by XR and XD might lead to redox imbalance, xylitol excretion,
and thus might reduce ethanol yield and productivity. In the present study, genes responsible for the conversion of xylose
to xylulose with different cofactor specificity (1) XR from N. crassa (NADPH-dependent) and C. parapsilosis (NADH-dependent), and (2) mutant XD from P. stipitis (containing three mutations D207A/I208R/F209S) were overexpressed in wild type yeast. To increase the NADPH pool, the fungal
GAPDH enzyme from Kluyveromyces lactis was overexpressed in the 424A(LNH-ST) strain. Four pentose phosphate pathway (PPP) genes, TKL1, TAL1, RKI1 and RPE1 from S. cerevisiae, were also overexpressed in 424A(LNH-ST). Overexpression of GAPDH lowered xylitol production by more than 40%. However, other
strains carrying different combinations of XR and XD, as well as new strains containing the overexpressed PPP genes, did not
yield any significant improvement in xylose fermentation. 相似文献