珍稀植物连香树在其中国分布区北缘的种子性状及幼苗更新限制

连香树(Cercidiphyllum japonicum)是第三纪孑遗植物, 存在严重的幼苗更新限制。为验证生活史早期(种子萌发)限制中国分布区北缘连香树种群幼苗更新, 并探讨其主要成因, 本研究在秦岭和太行山脉采集不同种源地的种子, 测定其形态性状、营养元素含量和质量、不同贮存时间的活力及不同温度条件下的萌发性状, 通过方差分析、相关分析等方法对不同种源地的种子性状进行分析。结果表明: 在中国分布区北缘, 其种子长度(P < 0.001)、萌发率(P < 0.001)、平均萌发时间(P < 0.001)、氮(P < 0.05)和磷含量(P < 0.001)在不同种源间存在显著差异; 而在区域尺度上(秦岭与太行山), 仅种子碳含量存在显著差异(P < 0.01)。天水种群的种子萌发率最高(21.77%), 平均萌发时间最长(11.12 d); 栾川的萌发率最低(1.38%), 平均萌发时间最短(3.47 d)。在25℃条件下, 济源种群的种子萌发率显著高于10℃、15℃和20℃条件下(P < 0.05), 而其他种源地的萌发率在不同温度条件下无显著差异。在4个温度条件下, 栾川种群种子的初始萌发时间无显著差异, 而其他4个种源地的初始萌发时间都随温度升高而缩短。相关分析结果表明, 种子萌发率与种子活力密切相关, 而种子活力与种子质量、种子的氮和磷含量显著相关。在中国分布区北缘, 连香树种子的自身属性(质量、氮和磷含量)通过影响种子活力间接影响萌发率; 且种子萌发对温度的响应主要表现在萌发时间上。本研究证实种子萌发是限制连香树种群幼苗更新的关键阶段, 主要原因如下: (1)连香树种子在9月成熟后, 10月的温度仍适宜种子萌发, 但较短生长期的幼苗在冬季低温下不能存活; (2)连香树种子萌发率低(14.4%); (3)第二年春天种子活力骤降。     

中国特有植物雪落樱桃潜在分布及其生态特征

为明确中国特有植物雪落樱桃(Cerasus xueluoensis)的潜在分布与居群生态特征,利用DIVA-GIS软件及其耦合的BIOCLIM模型,首次绘制了雪落樱桃适生区分布模拟图,并对影响其分布的主导气候因子进行了定性定量分析。结果表明,雪落樱桃当前潜在适生区主要分布在亚热带长江流域1 200 m以上高海拔山区,其中渝-鄂-湘三省交界的大巴山-巫山山脉可视为现代核心分布区,湘黔交界及湘南的南岭山脉可视为雪落樱桃潜在分布的南界,陕-豫-鄂交界山区的秦岭南麓可能是其潜在分布的北界。主成分分析(PCA)筛选的主导气候因子及其贡献率依次为:年降水量(bio12)最冷季降水量(bio19)最暖季降水量(bio18)最湿季降水量(bio16),累计频率曲线进一步确定其适宜范围分别为:993.00～1 870.22、500.00～680.00、430.00～669.16和500.00～680.00mm,表明降水是影响雪落樱桃当下分布格局的主导气候限制因子。Pearson相关性分析表明,雪落樱桃分布格局在区域尺度上受海拔、经、纬度影响;最小树分析和聚类分析表明,雪落樱桃7个野生居群可划分为中西部与东部两大分支;受试者工作特征曲线(ROC) AUC值达到0.751,满足模型预测精度的基本要求。这些有助于为雪落樱桃制定科学合理的资源保护与科学引种规划。     

云南48种木兰科野生植物资源遗传多样性研究

为了解云南省木兰科（Magnoliaceae）野生植物资源的遗传多样性,利用ISSR分子标记技术对48种木兰科野生植物资源进行研究。结果表明,10对引物共扩增出151条带,均为多态性条带,多态性条带百分率为100%。总的观测等位基因数（N_a）为2.000 0,有效等位基因数（N_e）为1.564 5,Nei’s基因多样性指数（H）0.337 9,Shannon’s信息指数（I）为0.510 1。总的基因多样性指数（H_t）为0.368 0,属间基因多样性指数（D_st）为0.251 9,占68.4%,基因分化系数（G_st）为0.684 0,基因流（N_m）为0.231 0。UPGMA聚类分析将48种木兰科植物划分为7个类群,各类群并非按照属聚在一起,而是不同属植物相间分布,长喙厚朴（Magnolia rostrata）、素黄含笑（Michelia flaviflora）和球花含笑（M.sphaerantha）可能为云南省木兰科植物中的原始种。48种木兰科野生植物总体具有较高的遗传多样性,但属间遗传变异较高,基因流较小,存在遗传漂变的风险,聚类结果与刘玉壶的分类系统存在分歧,这从分子水平为木兰科植物间的起源、进化与分类提供了重要依据。     

石灰碳铵熏蒸联合生物有机肥对香蕉枯萎病和细菌群落的影响

以连续种植的香蕉枯萎病高发病蕉园为试验点,通过实时定量PCR和高通量测序等方法,研究了田间条件下石灰碳铵熏蒸联合生物有机肥施用对香蕉枯萎病的防治效果,以及对土壤细菌群落结构和组成的影响。结果表明: 与不熏蒸施用有机肥(OF)相比,香蕉枯萎病发病率在施用有机肥前使用石灰碳铵进行熏蒸处理(LAOF)和施用生物有机肥前使用石灰碳铵进行熏蒸处理(LABF)中分别降低了13.3%和21.7%,病原菌的拷贝数分别降低了22.4%和33.0%。与OF处理相比,石灰碳铵熏蒸联合不同肥料施用均显著降低了细菌的丰富度和多样性,形成了明显不同的群落结构,且熏蒸对群落组成的差异产生了决定性的影响。LABF的细菌丰富度和多样性均低于其他处理,群落组成也与其他处理存在明显差异。与OF处理相比,熏蒸处理(LAOF和LABF)显著增加了土壤中水恒杆菌、布鲁式菌和漯河杆菌属的相对丰度,且在LABF中的相对丰度均高于LAOF,水恒杆菌和布鲁式菌的相对丰度差异显著。在田间条件下,施用生物有机肥之前使用石灰碳铵进行熏蒸处理能够显著降低病原菌数量,改变土壤细菌群落结构,激发土壤有益微生物,从而减少香蕉枯萎病的发生。     

Phenotypic and genetic dissection of cadmium accumulation in roots,nodes and grains of rice hybrids

Plant and Soil - Rice accumulates higher levels of cadmium (Cd) in grain than other cereals, and hybrid rice varieties cover around half of the total rice area in China. Therefore, understanding...     

The role of EMT-related lncRNA in the process of triple-negative breast cancer metastasis

Triple-negative breast cancer (TNBC) is the most malignant and fatal subtype of breast cancer, which has characterized by negativity expression of ER, PR, and HER2. Metastasis is the main factor affecting the prognosis of TNBC, and the process of metastasis is related to abnormal activation of epithelial–mesenchymal transition (EMT). Recent studies have shown that long non-coding RNA (LncRNA) plays an important role in regulating the metastasis and invasion of TNBC. Therefore, based on the metastasis-related EMT signaling pathway, great efforts have confirmed that LncRNA is involved in the molecular mechanism of TNBC metastasis, which will provide new strategies to improve the treatment and prognosis of TNBC. In this review, we summarized many signal pathways related to EMT involved in the transfer process. The advances from the most recent studies of lncRNAs in the EMT-related signal pathways of TNBC metastasis. We also discussed the clinical research, application, and challenges of LncRNA in TNBC.     

LncRNA AWPPH as a prognostic predictor in human cancers in Chinese population: evidence from meta-analysis

Background: Long non-coding RNA associated with poor prognosis of hepatocellular carcinoma (AWPPH) is dysregulated in a variety of human cancers. However, the prognostic value of AWPPH in various cancers remains unclear.Methods: Comprehensive literature search was performed in PubMed, Web of Science, CNKI and Wangfang databases, and eligible studies were obtained according to the inclusion and exclusion criteria. The pooled hazard ratios (HRs) and odds ratios (ORs) were applied to assess the clinical value of AWPPH expression for overall survival (OS) and clinicopathological features.Results: A total of 19 articles including 1699 cancer patients were included in the study. The pooled results demonstrated that evaluated AWPPH expression was positively related to a poorer overall survival of patients with cancers (HR = 1.79, 95%CI: 1.44–2.14, P<0.001). Subgroup analysis revealed that tumor type and sample size affect the predictive value of AWPPH on OS, whereas cut-off value and HR estimation method have no impact on it. In addition, the pooled data also showed that AWPPH was positively linked to advanced TNM stage (OR = 2.50, 95%CI: 1.94–3.22, P<0.001), bigger tumor size (OR = 2.64, 95%CI: 1.47–4.73, P=0.001), macro-vascular invasion (OR = 2.08, 95%CI: 1.04–4.16, P=0.04) and lymph node metastasis (OR = 2.68, 95%CI: 1.82–3.96, P<0.001). Moreover, the results of the trim and fill analysis confirmed the reliability of our finding.Conclusions: Up-regulation of AWPPH was associated with advanced TNM stage, bigger tumor size, worse lymph node metastasis, macro-vascular invasion and shorter overall survival, suggesting that AWPPH may serve as a biomarker for prognosis and clinicopathological characteristics in human cancers among the Chinese population.     

Cytotoxic,clonal fidelity,and antioxidant evaluation of in vitro propagated Vitex negundo var. cannabifolia

Plant Cell, Tissue and Organ Culture (PCTOC) - Vitex negundo L. var. cannabifolia (Sieb. et Zucc.) Hand.-Mazz. is an important medicinal plant rich in bioactive compounds. In vitro tissue culture...