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971.
972.
973.
Baoming?Wang Jianjun?Chen Longsheng?Chen Xiangnan?Wang Rui?Wang Li?Ma Shaofeng?Peng Jian?Luo Yongzhong?ChenEmail author 《Trees - Structure and Function》2015,29(5):1483-1492
Key message
Leaf relative water content, leaf area, leaf fresh weight, and SPAD chlorophyll meter readings along with Co - rbcL and Co - rbcS expression can be used for evaluating Camellia oleifera responses to combined drought and heat stress and subsequent recovery after rainfall events.Abstract
Leaf characteristics, soluble protein and total soluble sugar contents as well as Rubisco-related gene expression in three cultivars of C. oleifera were measured during a combined drought and heat stress period and after subsequent rainfall events. Leaf relative water content (RWC) was significantly correlated with leaf area (LA), leaf fresh weight (FW), SPAD chlorophyll meter readings, and the levels of Co-rbcL and Co-rbcS expression. Results suggest that leaf RWC, LA, leaf FW, SPAD readings together with Co-rbcL and Co-rbcS expression can be used for evaluating responses of C. oleifera cultivars to combined drought and heat stress and subsequent recovery after rainfall events. Rubisco activase might be used for evaluating plant recovery after rainfall. This study identified cultivars differing in tolerance to the combined stress and recovery. Information derived from this study should be valuable for improving survivability and productivity of C. oleifera cultivars.974.
975.
Brassinosteroids promote development of rice pollen grains and seeds by triggering expression of Carbon Starved Anther,a MYB domain protein 总被引:2,自引:0,他引:2
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Xiaolei Zhu Wanqi Liang Xiao Cui Mingjiao Chen Changsong Yin Zhijing Luo Jiaying Zhu William J. Lucas Zhiyong Wang Dabing Zhang 《The Plant journal : for cell and molecular biology》2015,82(4):570-581
Transport of photoassimilates from leaf tissues (source regions) to the sink organs is essential for plant development. Here, we show that a phytohormone, the brassinosteroids (BRs) promotes pollen and seed development in rice by directly promoting expression of Carbon Starved Anther (CSA) which encodes a MYB domain protein. Over‐expression of the BR‐synthesis gene D11 or a BR‐signaling factor OsBZR1 results in higher sugar accumulation in developing anthers and seeds, as well as higher grain yield compared with control non‐transgenic plants. Conversely, knockdown of D11 or OsBZR1 expression causes defective pollen maturation and reduced seed size and weight, with less accumulation of starch in comparison with the control. Mechanically, OsBZR1 directly promotes CSA expression and CSA directly triggers expression of sugar partitioning and metabolic genes during pollen and seed development. These findings provide insight into how BRs enhance plant reproduction and grain yield in an important agricultural crop. 相似文献
976.
Reticulate evolution and sea‐level fluctuations together drove species diversification of slipper orchids (Paphiopedilum) in South‐East Asia
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![点击此处可从《Molecular ecology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
South‐East Asia covers four of the world's biodiversity hotspots, showing high species diversity and endemism. Owing to the successive expansion and contraction of distribution and the fragmentation by geographical barriers, the tropical flora greatly diversified in this region during the Tertiary, but the evolutionary tempo and mode of species diversity remain poorly investigated. Paphiopedilum, the largest genus of slipper orchids comprising nearly 100 species, is mainly distributed in South‐East Asia, providing an ideal system for exploring how plant species diversity was shaped in this region. Here, we investigated the evolutionary history of this genus with eight cpDNA regions and four low‐copy nuclear genes. Discordance between gene trees and network analysis indicates that reticulate evolution occurred in the genus. Ancestral area reconstruction suggests that vicariance and long‐distance dispersal together led to its current distribution. Diversification rate variation was detected and strongly correlated with the species diversity in subg. Paphiopedilum (~80 species). The shift of speciation rate in subg. Paphiopedilum was coincident with sea‐level fluctuations in the late Cenozoic, which could have provided ecological opportunities for speciation and created bridges or barriers for gene flow. Moreover, some other factors (e.g. sympatric distribution, incomplete reproductive barriers and clonal propagation) might also be advantageous for the formation and reproduction of hybrid species. In conclusion, our study suggests that the interplay of reticulate evolution and sea‐level fluctuations has promoted the diversification of the genus Paphiopedilum and sheds light into the evolution of Orchidaceae and the historical processes of plant species diversification in South‐East Asia. 相似文献
977.
根据苦荞花期转录组数据,克隆得到1个苦荞bHLH类转录因子基因,命名为FtbHLH4。采用实时荧光定量PCR技术,分析了非生物胁迫对苦荞芽期FtbHLH4基因表达的影响。序列分析表明,苦荞FtbHLH4基因DNA全长1 852bp,由7个外显子和6个内含子构成,符合GT-AG剪接原则;cDNA序列包含1个1 062bp的开放阅读框,编码353个氨基酸,具有bHLH类蛋白典型的螺旋-环-螺旋保守结构域。在脱落酸(ABA)、NaCl和PEG模拟干旱胁迫下,苦荞芽期FtbHLH4基因表达量均持续上升,至48h时达最大,分别为胁迫前的11.3倍、12.0倍和6.1倍。而在冷胁迫和UV-B胁迫下,苦荞芽期FtbHLH4基因表达量迅速下降,分别于6h和12h降低至胁迫前的24%和23%。研究推测FtbHLH4基因以不同机制参与了苦荞对非生物胁迫的应答过程。 相似文献
978.
摘要:【目的】氨氧化古菌(Ammonia-oxidizing archaea,AOA)是奇古菌门中的唯一类群,广泛分布于各个生态系统中,对氮素生物地球化学循环起着重要作用。亚硝酸还原酶是反硝化作用的关键酶,目前关于AOA反硝化作用的研究较少,对AOA 亚硝酸盐还原酶基因多样性的研究有利于揭示AOA在反硝化中的作用。【方法】本研究以水体、沉积物和土壤为研究对象,构建了奇古菌样的nirK基因克隆文库,研究了这些环境中nirK相似基因的多样性。【结果】对奇古菌样的nirK基因文库及其序列分析表明:湖水及其沉积物的
nirK基因克隆文库得到10个OTUs,菜田土壤和水样则有8个OTUs;系统发育进化树表明这些nirK氨基酸序列和Candidatus Nitrosopumilus koreensis AR1,Nitrosopumilus maritimus SCM1最为相似,但相似度较低(53%-68%)。克隆文库多样性指数分析表明:所有样品都存在不同类型的nirK基因,水体样品nirK基因类型的多样性和均匀度高于土壤样品,菜田土壤的nirK基因类型多样性最高,分布最均匀。【结论】本研究表明土壤和淡水环境中奇古菌门nirK基因也具有较高的多样性,并且这些基因型与海洋样品差异非常大,这些基因编码的亚硝酸盐还原酶可能对这些环境中的反硝化作用有重要意义。 相似文献
979.
Establishment of Immortalized Mouse Bmp2 Knock‐Out Dental Papilla Mesenchymal Cells Necessary for Study of Odontoblastic Differentiation and Odontogenesis
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![点击此处可从《Journal of cellular physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
980.
Proteomic analysis of tilapia Oreochromis niloticus Streptococcus agalactiae strains with different genotypes and serotypes
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![点击此处可从《Journal of fish biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
L. Li Y. Shi R. Wang T. Huang W. Liang H. Luo X. Gan W. Huang J. Li A. Lei M. Chen 《Journal of fish biology》2015,86(2):615-636
Nine tilapia Oreochromis niloticus group B streptococcus (GBS) strains differing in serotype and genotype were selected and paired. Two‐dimensional difference gel electrophoresis (2D DIGE) and matrix‐assisted laser‐desorption ionization time‐of‐flight‐mass spectrometry (MALDI‐TOF‐MS) were used to analyse the protein profiles of the strain pairs. Forty‐three proteins corresponding to 66 spots were identified, of which 35 proteins were found in the seven selected strain pairs that represented pairs differing in genotype and serotype. Among the 35 proteins, numbers of differentially expressed proteins in strains of different serotypes were greater than found in strains of different genotypes, suggesting that serotype plays a more essential role than genotype in the differential protein expression among GBS strains. No distinct pattern was found with respect to genotype and the protein expression profile of GBS strains. Several proteins were identified as surface‐associated cytoplasmic proteins that possessed the typical immunity‐eliciting characteristics of surface proteins. The identified proteins were found to be involved in 16 biological processes and seven Kyoto encyclopaedia of genes and genomes (KEGG) pathways. The data, for the first time, identified differentially expressed proteins in O. niloticus GBS strains of different serotypes, which play a major role in immunogenicity of O. niloticus GBS than does genotype, offering further information for design of a vaccine against O. niloticus GBS. 相似文献