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871.
Lei-Lei Chen Tie Bin Zhu Hang Yin Jun Huang Lian Sheng Wang Ke Jiang Cao Zhi Jian Yang 《Molecular biology reports》2010,37(7):3067-3072
Endothelial nitric oxide synthase (eNOS) and nitric oxide (NO) may play an important role in attenuating cardiac remodeling
and apoptosis after myocardial infarction. However, the anti-inflammation effects of eNOS in infarcted myocardium and the
role of MAPK signaling in eNOS/NO mediated cardiac remodeling have not yet been elucidated. Adenovirus carrying Human eNOS
gene was delivered locally into heart 4 days prior to induction of myocardial infarction (MI) by left anterior descending
coronary artery ligation. Monocyte/macrophage infiltration was detected by ED-1 immunohistochemistry. Western blot was employed
to examine the activation of MAPK. eNOS gene transfer significantly reduced myocardial infarct size and improved cardiac contractility
as well as left ventricle (LV) diastolic function at 7 days after MI. In addition, eNOS gene transfer decreased monocyte/macrophage
infiltration in the infarct region of the heart. Phosphorylation of MAPK after MI were also dramatically reduced by eNOS gene
transfer. All the protective effects of eNOS were blocked by N(ω)-nitro-l-arginine methyl ester (L-NAME) administration, indicating a NO-mediated event. These results demonstrate that the eNOS/NO
system provides cardiac protection after MI injury through inhibition of inflammation and suppression of MAPK signaling. 相似文献
872.
Sweet flag (Acorus calamus L.) and yellow flag (Iris pseudacorus L.) have been used increasingly in constructed wetlands (CWs) for treatment of eutrophic wastewater. In order to properly match plant species with the type of wastewater being treated, it is important to know the performance of plant species under different NO3−/NH4+ ratios. We investigated dry matter (DW) production and N content of A. calamus and I. pseudacorus under five NO3−/NH4+ ratios (100/0, 75/25, 50/50, 25/75, and 0/100) in a hydroponic system. Results showed that the two species exhibited different preferences for NO3− and NH4+. Total DW, shoot DW, and N content were greater with NO3−/NH4+ ratios of 50/50 and 75/25 than otherwise for A. calamus, but these parameters were only higher under the sole NO3− treatment in I. pseudacorus. We conclude that A. calamus could be best used for treating wastewater in constructed wetlands with NO3−/NH4+ ratios between 50/50 and 75/25, while I. pseudacorus for treating wastewater with NO3− only to achieve the highest biomass production and efficiency in the removal of N. 相似文献
873.
874.
A female animal appears to approach an upper limit to the rate of sustained energy intake/metabolic rate (SusEI/MR) during lactation. However, different species of animals may respond differently to the sustainable limit. Here, we measured energy budget during lactation in female striped hamsters raising litters of natural size (Con), and females whose litter size was manipulated during early lactation to support fewer or more pups (minus pups, MP or plus pups, PP). The striped hamsters significantly decreased their body mass and increased food intake from early to late lactation; and MP females had lower weight loss and food intake than the control and PP females. Litter size of the PP group decreased significantly over the period of lactation, and pups were weaned at a similar weight to that of the controls. MP females supported a significantly lower litter mass throughout lactation compared with the control and PP females, but during late lactation the pups from the MP group were significantly heavier. Resting metabolic rate (RMR) did not differ significantly between the three groups and the gross energy intake during peak lactation was 5.0×, 4.2× and 5.0 × RMR for the control, MP and PP females, respectively. Female striped hamsters reached a plateau in food intake at around 14 g/d during peak lactation, which might signify a limit of SusEI at 5.0 × RMR. However, it was not possible to determine whether the limitation on SusEI was imposed centrally by the capacity of the gastrointestinal tract to process food, peripherally by the capacity of the mammary gland to produce milk, or by the capacity of animals to dissipate heat. 相似文献
875.
876.
Ricardo H. Flores Jiménez Marie‐Ange Do Cao Miyeon Kim David S. Cafiso 《Protein science : a publication of the Protein Society》2010,19(2):269-278
Site‐directed spin labeling (SDSL) was used to investigate local structure and conformational exchange in two bacterial outer‐membrane TonB‐dependent transporters, BtuB and FecA. Protecting osmolytes, such as polyethylene glycols (PEGs) are known to modulate a substrate‐dependent conformational equilibrium in the energy coupling motif (Ton box) of BtuB. Here, we demonstrate that a segment that is N‐terminal to the Ton box in BtuB, is in conformational exchange between ordered and disordered states with or without substrate. Protecting osmolytes shift this equilibrium to favor the more ordered, folded state. However, a segment of BtuB that is C‐terminal to the Ton box that is not solvent exposed is insensitive to PEGs. Protecting osmolytes also modulate a conformational equilibrium in the Ton box of FecA, with larger molecular weight PEGs producing the largest shifts in the conformational free energy. These data indicate that solvent‐exposed regions of these transporters undergo conformational exchange and that regions of these transporters that are involved in protein–protein interactions sample multiple conformational substates. The sensitivity to solute provides an explanation for differences seen between two high‐resolution structures of BtuB, which each likely represent one conformation from a subset of states that are normally sampled by the protein. This work also illustrates how SDSL and osmolytes may be used to characterize and quantitate conformational equilibria in membrane proteins. 相似文献
877.
878.
879.
Induced pluripotent stem (iPS) cell research has been growing a new height throughout the world due to its potentialities in medical applications. We can explore several therapeutic applications through the iPS cell research. In this review, we have first discussed the development of iPS cells, reprogramming factors, and effectiveness of iPS cells. Then we have emphasized the potential applications of iPS cells in pharmaceutical and medical sectors, such as, study of cellular mechanisms for spectrum of disease entities, disease-specific iPS cell lines for drugs discovery and development, toxicological studies of drugs development, personalized medicine, and regenerative medicine. 相似文献
880.
Maomao Zeng Yang Xiao Yizeng Liang Bing Wang Xian Chen Dongsheng Cao Hongdong Li Mei Wang Zhiguang Zhou 《Metabolomics : Official journal of the Metabolomic Society》2010,6(2):303-311
In this paper, a new strategy for processing GC/MS based metabolic profiling data via multivariate methods was proposed, which
is applied to a small pilot study of impaired fasting glucose. The data obtained from plasma samples of impaired fasting glucose
patients and healthy controls were first treated by principal component analysis and partial least squares-discriminant analysis
to explore the differences and discriminators of the two groups. Subsequently, correlation analyses were employed to examine
the relationships between blood glucose and the discriminators or their linear combination, thus may be considered as potential
biomarkers of impaired fasting glucose. The results showed that the metabolic patterns of the two groups were different. Furthermore,
eleven metabolites were screened as discriminators. Levels of nine of the eleven discriminators, say lactate, 2-ketoisocaproic
acid, alanine, α-hydroxyisobutyric acid, urea, phosphoric acid, α-glycerophosphoric acid, palmitic acid and stearic acid,
were found to be significantly higher in impaired fasting glucose patients, while 1-monopalmitin and 1-monostearin showed
the opposite trend. Correlation analysis indicated that 2-ketoisocaproic acid, stearic acid were positively, while 1-monopalmitin
and 1-monostearin were negatively correlated with blood glucose. Moreover, blood glucose correlated well with the linear combination
of the eleven discriminators by canonical correlation analysis. The results have demonstrated that 2-ketoisocaproic acid,
stearic acid and the linear combination of the eleven discriminators may be considered as the potential biomarkers of impaired
fasting glucose and the proposed method may be useful in a larger study for exploring the metabolic alterations and biomarker
candidates of diseases. 相似文献