新生大鼠辣椒素处理后脊髓内阿片受体的变化

本实验用受体放射自显影方法,研究了新生大鼠辣椒素皮下注射,成年后脊髓内３Ｈ－埃托菲（３Ｈ－ｅｔｏｒｐｈｉｎｅ）结合位点数的变化。发现新生鼠辣椒素处理可以引起脊髓后角浅层及中央管周围’Ｈ－ｅｔｏｒｐｈｉｎｅ结合位点明显减,其中Ⅰ、Ⅱ层自显影银粒数减少３６％左右。Ⅲ层减少２０％,中央管周围减少１１％。而前角及背外测束３Ｈ－ｅｔｏｒｐｈｉｎｅ结合位点数无显著性差异。结果表明脊髓内初级感觉传入细纤维上有阿片受体分布。本研究为探明阿片类物质在脊髓水平的痛觉调控机制提供一定的形态学依据。     

朱砂叶螨羧酸脂酶最优测试条件的选择

应用二次回归通用旋转组合设计,对朱砂叶螨离体羧酸酯酶测试过程中所需缓冲液pH值、恒温时间、反应温度及底物浓度,设立4因子5水平试验,在考虑4个因子主效应和互作效应的情况下,筛选测试羧酸酯酶的最优条件组合。     

准分子激光治疗近视眼

本文对准分子激光PRK治疗近视眼息者503例的早期临床结果进行分析,近视范围为－2．0至－15．0D,根据术前屈光度不同分为三组,术后随访三个月．结果证实准分子激光是一种安全、有效、预测性较好的治疗近视眼方法．全部病人提高了裸眼视力,绝大部分保持了最佳矫正视力,且没有产生危及视力的并发症．     

西藏纤恙螨属四新种（蜱螨亚纲：前气门目）

本记述了采自西藏小型动物体上的恙螨四新种．隶属恙螨科(Trombiculidae Ewing,1944)的纤恙螨属（Leptotrombitium Nagyio et al,1916)。模式标本保存于军事医学科学院微生物流行病学研究所医学昆虫标本馆,部分副模标本存第一作所在单位。     

一种东亚钳蝎碱性神经毒素的纯化和初步晶体学研究

经ＳｅｐｈａｄｅｘＧ－５０和ＳＰ－ＳｅｐｈａｄｅｘＣ－２５两次柱层析,从河南淅川马氏钳蝎毒素中分得一种碱性神经毒素ＢｍＫＭＩ８．等电聚焦和ＳＤＳ电泳显示单一组分,其ｐＩ为９．１,Ｍｒ为７１００．毒性测试结果表明,该组分对小白鼠有较强的毒性,对昆虫也有一定的毒性,已获得该毒素的两种晶型的大单晶,并测定其空间群为Ｐ２＿１２＿１２＿１,晶胞参数为：ＢｍＫＭＩ８－Ａ：ａ＝３６．７,ｂ＝２６．６,ｃ＝５２．０,ＢｍＫＭＩ８－Ｂ：ａ＝３６．６４Ａ,ｂ＝３７．３１,ｃ＝３７．９５,已收集了ＢｍＫＭＩ８－Ｂ分辨率为１．７４的Ｘ射线单晶衍射数据。     

Evidence for FUS6 as a component of the nuclear-localized COP9 complex in Arabidopsis.

The pleiotropic CONSTITUTIVE PHOTOMORPHOGENIC (COP), DEETIOLATED (DET), and FUSCA (FUS) loci are essential regulatory genes involved in the light control of seedling developmental patterns in Arabidopsis. Although COP1, DET1, COP9, and FUS6 (also called COP11) have been cloned, their biochemical activities and interactions remain elusive. We have recently suggested that multiple pleiotropic COP, DET, and FUS genes may encode subunits of a large regulatory complex. In this study, we generated specific antibodies against Arabidopsis FUS6 and show that accumulation of both COP9 and FUS6 is coordinated in the pleiotropic cop, det, and fus mutant backgrounds and in wild-type plants throughout development. Both COP9 and FUS6 cofractionated into identical high molecular mass fractions in an analytical gel filtration assay, and neither was found in its monomeric form. Moreover, antibodies raised against either COP9 or FUS6 selectively coimmunoprecipitated both proteins. We have also developed an Arabidopsis protoplast immunolocalization assay and demonstrated that the COP9 complex is localized in the nucleus and that its nuclear localization is not affected by light conditions or tissue types. The integrated genetic and biochemical results strongly support the conclusion that both COP9 and FUS6 are components of the nuclear-localized COP9 complex. Therefore, we have provided the strongest evidence for the conclusion that at least some of the pleiotropic COP, DET, and FUS loci act in the same signaling pathway.     

The Mouse Spam1 maps to proximal Chromosome 6 and is a candidate for the sperm dysfunction in Rb(6.16)24Lub and Rb(6.15)lAld heterozygotes

We have determined the chromosomal localization of the murine gene encoding the 68-kDa sperm adhesion molecule 1, Spam1 or Ph-20. Using two independent approaches, fluorescence in situ hybridization (FISH) and interspecific backcross analysis, we show that Spam1 maps to proximal mouse Chromosome (Chr) 6. This map position is within the conserved linkage group corresponding to human Chr 7q, where the human homolog, SPAM 1, has been shown to map previously. Genetic mapping shows the gene to be very closely linked to Met, one of the most proximal loci on MMU 6. It thus places the gene near the centromere and the junction of the Rb(6.16)24Lub and Rb(6.15)1Ald translocations. The essential role of the Spam1 sperm antigen in mouse sperm-egg interactions and its gene location provide strong support for its candidacy as the gene involved in the dysfunction of mouse sperm bearing the Rb(6.16)24Lub or Rb(6.15)1Ald translocation. Received: 16 July 1996 / Accepted: 23 September 1996     

粘虫颗粒体病毒的增效因子提高杆状病毒的感染

一株美洲粘虫GV(增效品系)在东方粘虫幼虫上进行增殖,所获粘虫GV(PuGv-Ps>对东方粘虫NPV(PsNPV)、棉铃虫NPV(HaNPV)和黄地老虎NPV(AsNPV)进行增效试验。 实验结果证明PuGV-Ps对三种NPV都有明显的增效作用, 其中以对PsNPV为最强,增效率达55%一85%;对HaNPV为30%-80%;而对AsNPV只有15%-35%。用凝胶过滤技术从PuGV-Ps中分离增效因子PusF-Ps,其对PsNPV和A,NPV的增效作用亦同样明显,250μg的PuSF-Ps可以提高PsNPV的感染能力达80%。经ScPhadcf-150柱(1.6X 90)和使用四种标准蛋白(细胞色素c、胃蛋白酶、牛血清清蛋白和乳酸脱氢酶)测得PuSF-Ps的分子量为160 000左右。     

一种免受乙醇干扰的GOD的酶电极

将酶电极应用于发酵糖的测定时。与糖共存的乙醇常常会影响测糖的准确性,通过对葡萄糖氧化酶(GOD)电极的研究,探讨了乙醇对测糖酶电极测定影响,进而研制出抗干扰的GOD酶电极,若是GOD电极的酶膜通过夹心法制备．在乙醇含量为0．1％(V／V)时·即产生显著的影响,使测定结果偏大4．3%,且乙醇的影响随浓度的升高而增大,若用尼龙网固定GOD膜,GOD电极在测定20mmol／L和5mm01／L左右的葡萄糖溶液时,含量高达9％的乙醇仍未对测定产生显著的影响．表现出良好的不受乙醇干扰的特性,并且,该尼龙网GOD电极具有良好的重复性、稳定的响应活性及较长的保存期．     

荸荠营养器官的发育与解剖学研究

荸荠同化茎起源于肉质主茎倒２或倒３叶的叶腋内。同化茎基部着生二鞘状叶,鞘状叶对早期同化茎穿出土面具保护作用。匍匐茎大多起源于同化茎基部鞘状叶的叶腋内。当植株开始抽生花茎时,地下匍匐茎顶端开始膨大。球茎的膨大是匍匐茎顶端５－６节的基本组织经细胞有丝分裂,增加细胞数目,然后由细胞体积的扩大来实现的。球茎具足够的营养物质供来年顶芽萌发的需要,故属水生植物冬芽的性质。