Do urinary mononuclear cells reflect disease activity in lupus nephritis?

Glomerular inflammation is associated with urinary mononuclear cells (UMC) in a number of diseases including IgA nephropathy and glomerulonephritis. We examined UMC from children with lupus nephritis for a number of years to characterize the types of mononuclear cells found in urine and to determine if they were associated with active lupus nephritis. Detailed analysis of UMC by cell counts and by flow cytometry showed that monocytes were the clearly dominant cell type. Evaluation of the smaller number of lymphocytes found in the urine of patients with active lupus nephritis demonstrated a strong predominance of CD8+ lymphocytes, in contrast to the normal CD4+/CD8+ ratio that is found in peripheral blood. The degree of proteinuria strongly correlated with the presence of UMC. The UMC counts decreased as their clinical condition improved as indicated by lower indices of flare. These observations suggest that UMC may be a valuable tool in detecting and monitoring disease activity in patients with severe lupus nephritis. More importantly, this study indicated that both monocytes and cytotoxic CD8+ T cells may play a role in pathogenesis of lupus nephritis.     

六种鼠苹果酸脱氢酶,血浆蛋白质和血红蛋白的变异分析

本文分析了家鼠属（Ｒａｔｔｕｓ）４种鼠和姬鼠属（Ａｐｏｄｅｍｕｓ）２种鼠的苹果酸脱氢酶、血浆中β１区和β２区蛋白质、血浆清蛋白、前清蛋白以及红细胞中血红蛋白的变异。结果表明,ＭＤＨ在进化上是比较保守的,其中ＭＤＨｍ和ＭＤＨｓ２带在各鼠间位于同一泳动线上,仅ＭＤＨｓ１带的泳动速度出现属间和种间的微小差异;血浆中β１区和β２区的蛋白质带、清蛋白带以及前清蛋白带各鼠间出现明显的变异,表现为黑线姬鼠和社鼠在β１区出现２种多态型区带,褐家鼠在β２区也有２种多态型带;各鼠间的血红蛋白变异比较明显,主要表现在区带数和主成分的泳动度显著不同,而且社鼠的Ｈｂ出现３种多态型,黄毛鼠出现２种多态型,其余各鼠的Ｈｂ皆为单态性。上述１６项生化特性按相似和相异进行配对比较,初步表明黄毛鼠与褐家鼠亲缘上比较相近,白腹巨鼠要比其他３种鼠亲缘上更接近于黑线姬鼠,而社鼠与白腹巨鼠之间进化分歧相对地较大。     

青海湖裸鲤寄生舌状绦虫的空间格局研究

舌状绦虫裂头蚴只寄生在体长小于２２０ｍｍ的青海湖裸鲤中,其种群在宿主种群中呈聚集分布。其聚集分布的强度随寄生虫种群平均密度的增加而降低。由于舌状绦虫在宿主体腔生长,产生明显的空间拥挤效应,可能导致宿主死亡或被食鱼鸟类捕食而转移到终末宿主中。在体长小于１２０ｍｍ的宿主中,密度依赖的死亡过程可能是使聚集强度降低的原因;而体长１４０－２００ｍｍ的鱼中,则是非密度依赖的全死过程使聚集强度增加．     

超氧化物歧化酶模拟化合物的生物活性研究

根据天然超氧化物歧化酶(SOD)活性部位结构合成了含苯并咪唑的5种配体及其32种分别含Cu（Ⅱ）、Fe（Ⅲ）、Mn（Ⅱ）、Co（Ⅱ）的模拟化合物.经光谱、电化学测试证明这些化合物具有拟S3D活性,其50％抑制率浓度（IC50）为10-6～10-8mol·L-1,催化超氧离子自由基（O-·2）歧化的反应速率常数kq为106～108mol-1·L·s-1.同时观察到几种模拟化合物有抗肿瘤活性或增强水稻抗寒性.     

检测香蕉花叶心腐病病原CMV的PAS—ELISA方法的改进

作为一种快速、敏感的免疫学方法,ELISA已被广泛地用于植物病毒的研究与应用性检测方面。在实验室中用PAS-ELISA检测香蕉花叶心腐病病原CMV已被证明是一种获得无CMV组培苗的有效手段。在香蕉无病毒组培苗工厂化大批量生产中,我们应用此法作为质量控制的手段之一,剔除病苗,保证产品不带CMV,获得一定成效。但同时我们也发现,香蕉组织中含有引起非特异性吸附反应的物     

三尖杉的组织培养－紫杉醇来源的新途径

从三尖杉当年生幼茎和未成熟假种皮诱导出了愈伤组织,对愈伤组织进行了培养研究,ＨＰＬＣ分析表明其紫杉醇和１０－ｄＡＢⅢ分别为细胞干重的０．００２０９％和０．０１２２％。三尖杉可望成为紫杉醇来源的新途径。     

Identification of a penicillin-binding protein 3 homolog, PBP3x, in Pseudomonas aeruginosa: gene cloning and growth phase-dependent expression.

A homolog of Pseudomonas aeruginosa penicillin-binding protein 3 (PBP3), named PBP3x in this study, was identified by using degenerate primers based on conserved amino acid motifs in the high-molecular-weight PBPs. Analysis of the translated sequence of the pbpC gene encoding this PBP3x revealed that 41 and 48% of its amino acids were identical to those of Escherichia coli and P. aeruginosa PBP3s, respectively. The downstream sequence of pbpC encoded convergently transcribed homologs of the E. coli soxR gene and the Mycobacterium bovis adh gene. The pbpC gene product was expressed from the T7 promoter in E. coli and was exported to the cytoplasmic membrane of E. coli cells and could bind [3H] penicillin. By using a broad-host-range vector, pUCP27, the pbpC gene was expressed in P. aeruginosa PAO4089. [3H]penicillin-binding competition assays indicated that the pbpC gene product had lower affinities for several PBP3-targeted beta-lactam antibiotics than P. aeruginosa PBP3 did, and overexpression of the pbpC gene product had no effect on the susceptibility to the PBP3-targeted antibiotics tested. By gene replacement, a PBP3x-defective interposon mutant (strain HC132) was obtained and confirmed by Southern blot analysis. Inactivation of PBP3x caused no changes in the cell morphology or growth rate of exponentially growing cells, suggesting that pbpC was not required for cell viability under normal laboratory growth conditions. However, the upstream sequence of pbpC contained a potential sigma(s) recognition site, and pbpC gene expression appeared to be growth rate regulated. [3H]penicillin-binding assays indicated that PBP3 was mainly produced during exponential growth whereas PBP3x was produced in the stationary phase of growth.     

甘蔗细平象的研究

甘蔗细平象Trockorhopalus humeralis Chevrolat是云南甘蔗上的一种毁灭性地下害虫,以幼虫和成虫在地下蔗头内为害,为害期8-10个月。据1989年调查,受害蔗每亩损失500-3000kg,严重的无收。此虫1年发生1代,以成虫在蔗头内越冬,有喜湿性,不能飞翔,主要通过沟河流水传播。在河川坝地,沙壤土中虫口较多;宿根年限越长的甘蔗受害越重。建议蔗稻轮作;缩短甘蔗宿根年限;早春翻挖有虫蔗蔸烧毁;结合新植蔗下种,宿根蔗松蔸培土施用甲基异柳磷或铁灭克等颗粒杀虫剂进行防治。