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排序方式: 共有294条查询结果,搜索用时 390 毫秒
111.
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Kun Luo Li Wang Xiaohu Chen Xiyang Zeng Shiyi Zhou Peicong Zhang Junfeng Li 《仿生工程学报(英文版)》2022,(1):167-178
3D porous scaffold could provide suitable bone-like structure for cell adhesion and proliferation;however,surgical suffer-ing from large volume implantation is ... 相似文献
113.
Trace element (TE) supplementation can reduce the incidence of multiple organ failure after severe trauma. The lung plays a main role in post-injury multiple organ failure. In the present study, the relationship between TEs and acute lung injury (ALI) post-injury was investigated in a rabbit model of severe trauma with an injury severity score of 27. New Zealand white rabbits were randomly assigned to trauma-control, trauma-TE groups, and a control group. During days 1-5 post-trauma, each rabbit in the trauma-TE group received 0.1 ml multi-TE compound intraperitoneally to give a daily dose of 32.50 mg/kg of Zn, 6.35 mg/kg of Cu, 1.38 mg/kg of Mn, and 0.16 mg/kg of Se. Concentrations of blood and lung selenium (Se), copper (Cu), zinc (Zn), and manganese (Mn) were measured at 6 and 24 h, as well as 3, 6, 9, and 14 days after trauma. Levels of glutathione peroxidase (GPx), total superoxide dismutase (SOD), Cu/Zn superoxide dismutase (Cu/Zn-SOD), and malondialdehyde (MDA) in serum and lung tissue and the level of intercellular adhesion molecular-1 (ICAM-1) in serum were detected simultaneously. In addition, the lung coefficient (LC) and the lung permeation index (LPI) were measured. Serum and lung Zn, Se, and Mn levels decreased dramatically by 6 h after trauma in both experimental groups. Cu showed no significant changes after trauma. The serum and lung GPx and SOD levels in the experimental group decreased significantly on days 1 and 3, respectively. Serum and lung MDA began to increase on day 3 in the trauma group but increased less after TE supplementation. Serum ICAM-1 peaked on day 6 in the experimental group. LC and LPI increased gradually post-trauma, peaking on days 6 and 9, respectively. In conclusion, an acute lung injury causes declines of the levels of TEs in serum and lung which can be significantly prevented by TE supplementation and which can also mitigate some of the morphological and biomechanical changes in ALI. 相似文献
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不同浓度海水对菊芋幼苗生长及生理生化特性的影响 总被引:10,自引:0,他引:10
种植抗盐耐海水植物是合理利用和开发海涂资源的有效措施之一。采用水培的方式,用1/2Hoagland营养液培养菊芋幼苗至6叶完全展开时进行处理,设0%(对照)、10%、25%和50%海水4个处理。随后分别在第4、8和12天采样进行分析,研究不同浓度海水对菊芋幼苗生长、体内渗透物质的积累、保护性酶活性、膜透性及离子吸收分布的影响情况。结果表明:(1)在不同浓度海水处理下,菊芋地上部、地下部总鲜重及干物质重从0%到25%海水浓度没有明显变化,在50%海水胁迫下显著下降,干物质百分比则为50%海水处理的最高。随着时间延长,10%海水处理下,菊芋幼苗茎叶和根鲜重均增加,但与对照没有显著差异,25%海水处理生长速率较对照低,而50%海水处理下根鲜重和干重都降低。(2)随着时间的延长、海水浓度的增加,菊芋幼苗叶片保护性酶系SOD、POD、CAT的活性呈上升趋势,在10%海水处理下膜脂过氧化物MDA含量甚至低于对照,而50%海水处理下的MDA含量较其他处理高,在10%和25%海水处理下膜透性较对照变化不显著,而50%海水处理下膜透性增加明显,且随时间延长更显著。(3)菊芋幼苗叶片脯氨酸和可溶性糖含量随海水浓度增高而显著增加,随着时间的延长,10%和25%海水处理下,脯氨酸含量先增加后降低,而50%海水处理下,脯氨酸含量一直在升高,而10%、25%和50%海水处理下,可溶性糖含量先增加后降低。随海水浓度增高,菊芋幼苗地上部单位干重积累的Na 和Cl-依次增大,且随着时间延长,10%、25%和50%海水处理下地上部Na 和Cl-含量均增大;而K 与Na 积累情况不同,K 在25%海水胁迫下地上部单位干重积累得最多,随着时间延长,25%和50%海水处理下地上部K 含量均降低,且50%海水处理下降低幅度更大;地下部单位干重积累的Na 、Cl-和K 情况与地上部单位干重积累的各离子趋势相似。由此可见,菊芋能够通过生理生化机制适应一定浓度海水的灌溉,即利用一定浓度海水灌溉菊芋是安全有效的。 相似文献
116.
树轮记录的贺兰山区近百年来的干旱事件 总被引:6,自引:0,他引:6
利用采自东亚夏季风最北缘、地处干旱-半干旱地区的贺兰山区的树轮样芯,建立了贺兰山地区最近93年来的树轮宽度年表。与气象观测记录的相关分析结果表明,降水是限制贺兰山区树木生长的主要因素,其中5~7月份的降水与树轮宽度呈显著正相关关系,相关系数为0.522(通过95%的信度检验)。在贺兰山的树轮宽度记录中有两个主要的低生长期即20世纪20年代和70年代末到90年代末,这两个低生长期均与该区域的干旱事件相对应。通过分析还发现在干旱事件中不仅出现低降水而且同时与高气温相伴。也就是说在干旱时期内,高温和低降水的水热组合对树木影响十分显著,从而由单纯降水减少变为一种低降水高蒸发的环境,加剧了气候的干旱程度从而使树木生长出现低的生长期,形成窄轮。这种水热组合引起树轮宽度的变化对于理解过去干旱事件及其过程具有重要意义。 相似文献
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We describe a simple protocol to achieve homonuclear J-decoupling in the indirect dimensions of multidimensional experiments, and to enhance spectral resolution of the backbone Calpha carbons in the 3D NCACX experiment. In the proposed protocol, the refocusing of the Calpha-CO homonuclear J-couplings is achieved by applying an off-resonance selective pi pulse to the CO spectral region in the middle of Calpha chemical shift evolution. As is commonly used in solution NMR, a compensatory echo period is used to refocus the unwanted chemical shift evolution of Calpha spins, which takes place during the off-resonance selective pulse. The experiments were carried out on the beta1 immunoglobulin binding domain of protein G (GB1). In GB1, such implementation results in significantly reduced line widths, and leads to an overall sensitivity enhancement. 相似文献
119.
Lipid biomarkers were investigated to reveal the microbial life preserved in sulfide and Si-rich chimney from the 49.6°E hydrothermal vent field. In sulfide chimney, iso-/anteiso-fatty acids and H-shaped glycerol dialkyl glycerol tetraethers are the main microbial biomarkers. In Si-rich chimney, monounsaturated fatty acids (C16:1n7, C18:1n7) are the main bacterial biomarkers detected, and crenarchaeol and its isomer are relatively abundant (up to 25% of glycerol dialkyl glycerol tetraethers) archaeol biomarkers. Composition of lipid biomarkers reveals the diversity of microbial communities in different types of chimney structures. Sulfate-reducing bacteria and hyperthermophilic archaea were considered to be the majority microbial life in sulfide chimney, and sulfur-oxidizing bacteria were abundant in Si-rich chimney while archaea in Si-rich chimney and mainly attributed to Thaumarchaeota, which were predominately ammonia oxidizers. Our result suggested that fluid temperature and gaseous components could be the main constraints for the diversity of microbial communities in hydrothermal chimney structures in 49.6°E hydrothermal vent field. 相似文献
120.
采用生物素化凝集素ConA、RCA-I、UEA-I、PNA、SBA 和WGA, 对大鼠左室壁血管内皮的凝集素受体进行了观察, 结果发现, 大鼠左室壁血管内皮存在有ConA、RCA-I及WGA受体, 文中讨论了血管内皮凝集素受体的分布情况及其意义。 相似文献