Nogo—p4与NgR结合抑制神经干细胞分化为双极形星形胶质细胞的突起生长

目的观察Nogo—p4是否通过与NgR结合的途径对大鼠脊髓来源神经干细胞分化形成双极形星形胶质细胞突起长度产生抑制。方法取4只出生24h内的Wistar大鼠,悬浮培养法培养大鼠脊髓来源的神经干细胞。把神经干细胞分为A、B、C、D四组,A组加入血清,B组加入血清和Nogo—p4,C组神经干细胞经RNA干扰沉默NgR基因后加入血清分化,D组神经干细胞经RNA干扰沉默NgR基因后加入血清和Nogo—p4。分化第7d,GFAP抗体标记星形胶质细胞,使用Image—ProPlus5．0软件测量双极形星形胶质细胞突起长度。结果神经干细胞分化第7d,四组均可形成双极形星形胶质细胞。B组中双极形星形胶质细胞的突起长度明显短于其它各组。A、C、D组中双极形星形胶质细胞的突起长度没有显著差异。结论Nogo—p4经与NgR结合途径显著抑制脊髓来源神经干细胞分化成的双极形星形胶质细胞的突起生长。     

大连地区感染性腹泻病原监测结果分析

目的 了解大连地区感染性腹泻病原菌的种类和流行特征,为感染性腹泻的预防控制和临床治疗提供依据.方法 采用常规粪便培养方法,对分离菌做血清学分型,用MicroScan WalkAway-40全自动细菌鉴定及药敏分析仪对分离菌做生化鉴定,对检出的志贺菌做药敏试验,并用WHONET 5.4软件对药敏结果进行统计分析.结果 监测872例腹泻患者样本,检出感染性腹泻病原菌109株,阳性率为12.5％,其中志贺菌所占比例最大,其次为沙门菌,副溶血弧菌排第三.志贺菌对碳青霉烯类、氟喹诺酮类和头孢菌素类等敏感,对青霉素类和复方磺胺类耐药.结论 志贺菌是大连地区感染性腹泻的首要病原菌,沙门菌和副溶血弧菌次之,应依据此监测结果做好大连地区腹泻病的防治工作;同时根据耐药性监测结果合理使用抗生素应对志贺菌,减少耐药菌株出现.     

308nm准分子激光治疗白癜风皮肤病进展

临床研究表明波长308nm准分子激光治疗白癜风皮肤病效果明显。治疗效果与病人的性别、年龄、病程没有明显关联,但与皮肤类型、皮损部位、治疗频度和疗程等有明显关联。在一定剂量范围内随治疗剂量和治疗时间增加治疗效果越来越明显,呈线性变化。而皮损部位的影响,疗效从明显到不明显的顺序是：面部,颈部和头皮,生殖器,四肢,躯干,手脚或肢端关节。皮肤类型对治疗效果影响明显。另外,在一定的观察时间内发现308nm准分子激光疗效明显高于NB UVB。     

瑞舒伐他汀治疗颈动脉粥样硬化斑块的临床研究

目的:研究瑞舒伐他汀对颈动脉粥样硬化斑块的治疗效果。方法:将在本院接受治疗的250例颈动脉粥样硬化斑块患者随机分成治疗组125例和对照组125例,治疗组服用瑞舒伐他汀10mg/晚,对照组行其他非瑞舒伐他汀药物治疗,进行为期6个月的观察对比。结果:治疗组治疗后总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)水平显著下降,高密度脂蛋白(HDL)水平显著升高(P<0.05),颈动脉内膜-中层厚度(IMT)、斑块面积变小,与治疗前比较,差异有统计学意义(P<0.05);对照组治疗前后无显著性差异(P>0.05)。结论:瑞舒伐他汀对降低血脂、减缓不稳定型心绞痛早期动脉粥样硬化、稳定斑块和预防脑血管疾病起到非常重要的作用。     

气囊漏气实验预测严重上气道狭窄的临床意义

目的:观察气囊漏气实验预测拔管后发生严重上气道梗阻的临床价值.方法:共有156例气管插管患者入组,根据气囊漏气实验的情况分为两组,阴性组和阳性组,拔除气管插管后观察两组喘鸣发生与二次气管插管情况.结果:156例患者中气囊漏气实验阴性组125例,阳性组31例,拔除气管插管后,有13例患者需再次插管,其中1例患者无法插管,予经皮气管切开.结论:气囊漏气实验是一项简单方便筛查拔管后可能上气道梗阻的方法,如为阴性其因严重上气道梗阻再插管可能性很小,如为阳性则要注意可能发生拔管后上气道梗阻.     

汶川地震救援人员创伤后应激反应特征的初步研究

目的:探讨5.12汶川地震军队救援人员创伤后应激损伤状况。方法:采用创伤后压力诊断量表、焦虑自评量表、抑郁自评量表和匹兹堡睡眠质量问卷,在地震爆发后65天左右,对107名执行运输遗体任务的官兵进行了测量。结果:PTSD症状轻度的占84%,中重度的占15%。其中症状在1个月内消失的占63%。从症状的分布来看,闯入性回忆占36.4%,回避29.9%,过度警觉29.9%,焦躁不安或易怒37.4%,入睡困难、睡眠易醒或早醒43.9%,难以集中注意力45.8%,对重要的活动兴趣下降32.7%。救援人员抑郁症状得分低于全国常模水平;焦虑自评量表得分与全国常模差异不显著。睡眠质量差的占72%,以入睡时间长和日间功能降低为主。结论:担任遗体运输的救援官兵在救援早期普遍存在创伤后应激反症状,症状程度较轻,多数在1个月内消失,焦虑和抑郁症状水平的总体状况也比较低,但普遍存在睡眠质量差的问题,并且直接影响到日间功能。     

对胃镜检查患者实施图片成像干预的临床研究

目的:探讨图片成像干预对胃镜检查患者实施护理的临床效果。方法:选取50例胃镜检查患者,随机分为照组和试验组,其中对照组25例,给予常规护理;试验组25例,在对照组基础上采用图片成像进行护理干预。观察两组患者相关知识的掌握、操作时的配合程度、护士的解释时间、完成操作时间以及患者的满意率。结果:试验组实施干预后,患者相关知识的掌握、操作时的配合优于对照组(P<0.01),护士的解释时间与操作完成时间明显短于对照组(t=7.788/2.887,P<0.01),患者满意度明显提高(X2=4.73,P<0.05)。结论:图片成像干预有助于胃镜检查患者掌握相关疾病知识及操作时配合,利于操作的进行,提高护理质量。     

Differences in mitochondrial gene expression profiles, enzyme activities and myosin heavy chain types in yak versus bovine skeletal muscles

Hypoxia can affect energy metabolism. We examined gene expression and enzyme activity related to mitochondrial energy metabolism, as well as myosin heavy chain (MyHC) types in yaks (Bos grunniens) living at high altitudes. Real-time quantitative PCR assays indicated that the yak has significantly lower levels of carnitine palmitoyltransferase (CPT) mRNA in the biceps femoris and lower levels of uncoupling protein 3 (UCP3) mRNA in both biceps femoris and longissimus dorsi than in Yellow cattle. No significant differences between yak and Yellow cattle were observed in the activities of mitochondrial β-hydroxyacyl-CoA dehydrogenase, isocitrate dehydrogenase and cytochrome oxidase in the same muscles. Semi-quantitative RT-PCR analysis showed that the MyHC 1 mRNA levels in yak biceps femoris was lower than in Yellow cattle. We conclude that the yak has significantly lower mRNA levels of CPT, UCP3, and MyHC 1 in biceps femoris than in Yellow cattle, suggesting that the yak biceps femoris has lower fatty acid oxidation capacity and greater glycolytic metabolic potential.     

A Novel Rabbit Immunospot Array Assay on a Chip Allows for the Rapid Generation of Rabbit Monoclonal Antibodies with High Affinity

Antigen-specific rabbit monoclonal antibodies (RaMoAbs) are useful due to their high specificity and high affinity, and the establishment of a comprehensive and rapid RaMoAb generation system has been highly anticipated. Here, we present a novel system using immunospot array assay on a chip (ISAAC) technology in which we detect and retrieve antigen-specific antibody-secreting cells from the peripheral blood lymphocytes of antigen-immunized rabbits and produce antigen-specific RaMoAbs with 10^–12 M affinity within a time period of only 7 days. We have used this system to efficiently generate RaMoAbs that are specific to a phosphorylated signal-transducing molecule. Our system provides a new method for the comprehensive and rapid production of RaMoAbs, which may contribute to laboratory research and clinical applications.