乳酸菌素片联合标准四联疗法对社区消化性溃疡患者胃电图及肠道菌群的影响

目的探讨乳酸菌素片结合标准四联疗法对社区消化性溃疡(PU)患者胃电图及肠道菌群的影响,为该类患者的治疗提供参考。方法选取2018年1月至2020年3月社区PU患者128例,随机分为对照组和试验组,各64例。对照组患者给予标准四联疗法,试验组在对照组基础上给予乳酸菌素片,两组患者均治疗14 d。观察两组患者H.pylori根除率、治疗效果、再生黏膜组织学成熟度、不良反应及治疗前后胃电图(胃肠电节律紊乱、平均频率)、胃肠激素[胃蛋白原Ⅰ(PGⅠ)、生长抑素(SS)、生长激素释放多肽(Ghrelin)]、肠道菌群(革兰阳性球菌、革兰阴性球菌、革兰阳性杆菌)水平,并于治疗后3个月随访PU复发率。结果试验组患者治疗总有效率(95.31%)、H.pylori根除率(87.50%)及再生黏膜组织学成熟度均高于对照组(均P0.05)。胃电图检测显示,治疗后试验组患者餐前、餐后平均频率及胃肠电节律紊乱均低于对照组(均P0.05)。治疗后试验组患者血清SS水平高于对照组,PGⅠ、Ghrelin水平低于对照组(均P0.05)。治疗后试验组患者肠道革兰阴性球菌数量高于对照组,革兰阳性杆菌数量低于对照组(均P0.05)。两组患者不良反应发生率及PU复发率比较差异无统计学意义(均P0.05)。结论乳酸菌素片结合标准四联疗法治疗社区PU患者的疗效确切,可有效调控患者胃肠激素,提高H.pylori根除率,安全性较高。     

香茅精油的抑菌和抗氧化活性研究

选用产自福建厦门的香茅(Cymbopogon nardus),测定香茅精油对白色粘性沙雷氏菌、红色粘性沙雷氏菌、大肠杆菌、金黄葡萄球菌、铜绿假单胞菌和紫色杆菌等6种菌的抑菌活性,并利用ABTS^.+自由基清除法以及超氧阴离子清除法研究香茅精油的抗氧化活性。结果表明,新鲜香茅精油的平均提取率为0.29,对6种供试菌种都有较高的抑制作用,其中对紫色杆菌的抑制活性最强,且香茅精油有极高的清除ABTS^.+自由基以及超氧阴离子自由基的能力,证明香茅精油具有广谱抑菌活性及强抗氧化活性。     

Establishment of a micropropagation supporting technology for the Fraxinus mandshurica × Fraxinus sogdiana

In Vitro Cellular & Developmental Biology - Plant - The interspecific hybridization can take advantage of heterosis and combine the double parental traits most extensively, and is an effective...     

生防菌株HG18基因组信息分析与抗菌功能基因挖掘

贝莱斯芽胞杆菌（Bacillus velezensis）HG18是1株低温生防菌株,能够分泌抗菌物质。为挖掘和利用其抗菌功能基因,服务农业生产,采用二、三代相结合测序技术,对其进行全基因组测序,获得菌株完整基因组序列。基因组全长4 461 844 bp,包含一个染色体和一个质粒,GC含量44.06%,编码4 643个基因,编码基因总长度3 893 994 bp,占基因组87.27%。发现6个几丁质降解相关基因,2个葡聚糖酶基因和1个壳聚糖酶基因,2个脂肽类抗菌物质芬芥素与表面活性素合成基因簇,2个细菌素subtilin和bacillolysin合成基因。研究为提高抗菌物质产量的菌株定向遗传改造以及植物抗病育种提供基因资源。     

Procyanidin B1 promotes in vitro maturation of pig oocytes by reducing oxidative stress

Oxidative stress negatively affects the in vitro maturation (IVM) of oocytes. Procyanidin B1 (PB1) is a natural polyphenolic compound that has antioxidant properties. In this study, we investigated the effect of PB1 supplementation during IVM of porcine oocytes. Treatment with 100 μM PB1 significantly increased the MII oocytes rate (p <0.05), the parthenogenetic (PA) blastocyst rate (p <0.01) and the total cell number in the PA blastocyst (p < 0.01) which were cultured in regular in vitro culture (IVC) medium. The PA blastocyst rate of regular MII oocytes activated and cultured in IVC medium supplemented with 100 and 150 μM PB1 significantly increased compared with control (p < 0.01 and p < 0.05). We also evaluated the reactive oxygen species (ROS) levels, mitochondrial membrane potential (Δψm) levels, glutathione (GSH) levels, and apoptotic levels in MII oocytes and cumulus cells following 100 μM PB1 treatment. The results showed that the PB1 supplementation decreased ROS production and apoptotic levels. In addition, PB1 was found to increase Δψm levels and GSH levels. In conclusion, PB1 inhibited apoptosis of oocytes and cumulus cells by reducing oxidative stress. Moreover, PB1 improved the quality of oocytes and promoted PA embryo development. Taken together, our results suggest that PB1 is a promising antioxidant additive for IVM of oocytes.     

Numerical Study of the Dynamics of an Electron Avalanche in the Forming Cathode Sheath of a Self-Sustained Volume Discharge

Plasma Physics Reports - The dynamics of an electron avalanche starting from the flat cathode surface and from the tops of microtips of different heights on the cathode surface is compared. The...     

Effect of Fish on the Transfer Efficiency of Carbon,PUFA, and Nutrients from Phytoplankton to Zooplankton under Eutrophic Conditions

Biology Bulletin - The efficiency of the transfer of carbon, polyunsaturated fatty acids (PUFA), nitrogen, and phosphorus from phytoplankton to zooplankton determines the functioning of the entire...     

Features of the Radial and Axial Distributions of the Toroidal Magnetic Field in the Axial Jet Ejection at the PF-3 Facility

Plasma Physics Reports - The results of experiments on the study of the features of the dynamics and internal structure of the axial plasma ejection at the PF-3 facility carried out within the...