Two new <Emphasis Type="Italic">Entoloma</Emphasis> s.L. species with serrulatum-type lamellar edge from Changbai Mountains,Northeast China

Two species of Entoloma with serrulatum-type lamellar edge, E. cyanostipitum and E. subcaesiocinctum from the Changbai Mountains in northeast China, are described as new. E. cyanostipitum is distinguished by a brownish orange pileus with a central depression, black-blue lamellar edge, deep blue stipe, and cylindrical cheilocystidia with intense blue, intracellular pigment. E. subcaesiocinctum is characterized by a depressed pileus covered with brownish squamules, blackish blue lamellar edge, and cylindrical to clavate cheilocystidia with intense blue intracellular pigment. The two new species are further confirmed based on the ITS and nLSU sequences.     

山西西部吕梁山中北段现代花粉散布特征

山西西部吕梁山中北段关帝山和芦芽山地区7个不同植被类型共计15个表土样品和7个捕捉器样品表土花粉组合特征和花粉通量研究表明:(1)表土和捕捉器样品花粉组合中主要类型与植被组成中优势类型一致.(2)关帝山与芦芽山现代花粉组合特征差异明显,芦芽山地区花粉组合中草本花粉占优势,百分比多高于50%,乔木花粉百分比多低于50%,花粉通量平均为27194 grains/(cm2 · a);关帝山乔木花粉占优势, 百分比多高于50%,草本花粉多低于30%,花粉通量平均为57961 grains/(cm2 · a).(3)松属、云杉属和蒿属花粉在表土样品中的百分比远高于捕捉器样品,显示较强的保存能力;落叶松属、桦属和栎属花粉在表土样品中的百分比明显低于捕捉器样品,显示花粉保存能力相对较差.(4)样品聚类分析和DCA分析结果显示:二者分析结果具有很好的一致性,花粉组合最主要的控制因子是温度.     

黏着斑激酶与细胞迁移

细胞迁移过程始于细胞前端板状伪足的形成、外周黏附的建立、细胞体的收缩和尾部的解离.黏着斑激酶是一种非受体酪氨酸蛋白激酶,通过其激酶活性和"脚手架"的功能在细胞迁移的各个过程中发挥关键作用.现重点介绍黏着斑激酶介导的信号转导通路及其在调控细胞迁移方面的研究进展.     

Flow injection fluorescence immunoassay for gentamicin using sol-gel-derived mesoporous biomaterial

Sol-gel-derived mesoporous biomaterials were used for the first time in the flow-injection fluorescence immunoassay system. Anti-gentamicin antibody was immobilized in a mesoporous sol-gel material using tetramethoxysilane as a precursor and poly(ethylene glycol) as a template. The sol-gel glass was used to develop an immunoaffinity column for the flow-injection immunoassay of gentamicin. Little unspecific adsorption of gentamicin on the sol-gel and no antibody leaching under harsh elution conditions were found. The immunoassay is based on the competition between gentamicin and fluorescein isothiocyanate-labeled gentamicin for a limited number of encapsulated antibody binding sites. NaOH solution of 5 x 10(-3)mol/L is used for the regeneration of encapsulated antibody binding sites after each measurement, which allows the immunoreactor to be used for up to 20 times without any loss of reactivity. Sample preconcentration is not needed and a single assay can be performed within 10 min. The calibration for gentamicin has a working range of 250-5000 ng/mL with a detection limit of 200 ng/mL, which is close to that of the fluorescence immunoassay and fluorescence polarization immunoassay using the same reactants. Comparison of the results from this method with that obtained from HPLC showed an excellent correlation.     

丹参单体IH764-3对H2O2刺激肝星状细胞增殖和胶原合成的影响及其机制

目的：IH764－3对H2O2刺激的大鼠肝星状细胞（HSCs)增殖及胶原合成的抑制作用以及对粘着斑激酶（FAK）的影响,旨在为临床防治肝纤维化提供理论依据。方法：以不同剂量IH764－3干预H2O2刺激的HSCs,通过^3H-胸腺嘧啶（^3H-TdR)、^3H－脯氨酸（^3H-pro)掺入法测定HSCs增殖及胶原合成能力,应用逆转录聚合酶链反应（RT－PCR）方法检测FAK mRNA。结果：不同剂量IH764－3（10μg/ml,20μg/ml,30μg/ml,40μg/ml）作用于HSCs 48h及30μg/ml IH764－3作用于HSCs不同时间（12h,24h,48h),与单纯H2O2组相比,HSCs增殖明显被抑制（P＜0．05）;胶原合成能力降低（P＜0．05）;同时FAK mRNA表达下降。结论：丹参单体IH764－3能够抑制H2O2刺激的HSCs增殖及胶原合成,下调FAK是IH764－3抑制HSCs增殖及胶原合成的分子机制之一。     

A recombinant pseudorabies virus co-expressing capsid proteins precursor P1-2A of FMDV and VP2 protein of porcine parvovirus: a trivalent vaccine candidate

Pseudorabies (PR), foot-and-mouth disease (FMD), and porcine parvovirus disease are three important infectious diseases in swine worldwide. The gene-deleted pseudorabies virus (PRV) has been used as a live-viral vector to develop multivalent genetic engineering vaccine. In this study, a recombinant PRV, which could co-express protein precursor P1-2A of FMDV and VP2 protein of PPV, was constructed using PRV TK⁻/gE⁻/LacZ⁺ mutant as the vector. After homologous recombination and plaque purification, recombinant virus PRV TK⁻/gE⁻/P1-2A-VP2 was acquired and identified. Immunogenicity, safety of the recombinant PRV and its protection against PRV were confirmed in a mouse model by indirect ELISA and serum neutralization test. The results show that the recombinant PRV is a candidate vaccine strain to develop a novel trivalent vaccine against PRV, FMDV and PPV in swine.     

The consequences of reducing expression of the alpha7 nicotinic receptor by RNA interference and of stimulating its activity with an alpha7 agonist in SH-SY5Y cells indicate that this receptor plays a neuroprotective role in connection with the pathogenesis of Alzheimer's disease

In order to examine the neuroprotective effects of the alpha7 nicotinic receptor (nAChR) in relationship to the pathogenesis of Alzheimer's disease (AD), neuroblastoma (SH-SY5Y) cells were transfected with small interference RNAs (siRNAs) that targets specifically towards alpha7 nAChR or exposed to 20microM 3-[2,4-dimethoxybenzylidene] anabaseine (DMXB), a selective agonist of this same receptor. The levels of alpha7 nAChR mRNA and protein were measured by RT-PCR and Western blotting, respectively. The levels of the alpha-form of secreted amyloid precursor protein (alphaAPPs), total APP and the extracellular signal-regulated kinase 1/2 (ERK1/2) were also determined by Western blotting. SH-SY5Y cells transfected with siRNA or exposed to DMXB were then treated with 1microM Abeta(25-35), following which the levels of lipid peroxidation and rate of reduction of MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] were characterized by utilizing spectrophotometric procedures. Compared to controls, SH-SY5Y cells transfected with siRNA expressed the decreases in the levels of alpha7 nAChR mRNA and protein by 81% and 69% lower levels, respectively; exhibited reduced levels of the alphaAPPs and ERK1/2 proteins; and demonstrated enhanced lipid peroxidation and a decreased rate of MTT reduction. In cells exposed to DMXB, the level of alpha7 nAChR protein was elevated by 23%, with no alteration in the content of the corresponding mRNA; the levels of the alphaAPPs and ERK1/2 proteins were increased. Inhibition of the expression of the alpha7 nAChR gene enhanced the toxicity exerted by Abeta, whereas stimulation of this receptor attenuated this toxicity exerted. These findings indicate that alpha7 nAChR may play a significant neuroprotective role by enhancing cleavage of APP by alpha-secretase, regulating signal transduction, improving antioxidant defenses and inhibiting the toxicity of Abeta, which is connected with the pathogenesis of AD.     

Effects of mercuric ion on the conformation and activity of Penaeus Vannamei beta-N-acetyl-d-glucosaminidase

beta-N-acetyl-d-glucosaminidase (NAGase, EC.3.2.1.52), a composition of the chitinases, catalyzes the cleavage of N-acetylglucosamine polymers into N-acetylglucosamine. In this paper, the effects of mercuric ion on the activity of NAGase from Penaeus vannamei for the hydrolysis of pNP-NAG have been studied. The results show that HgCl2 can lead to irreversible inactivation to this enzyme. The inactivation process follows a first-order reaction and the inactivation rate constants have been determined. The relationship between the inactivation rate constants and HgCl2 concentration has been studied and the result shows that only one molecule of HgCl2 binds to the enzyme molecule to lead the enzyme lose its activity. Moreover, the conformational changes of the enzyme inactivated by HgCl2 were studied by following changes in the intrinsic fluorescence emission and ultraviolet absorption spectra.     

Pathogen elicitor-induced changes in the maize extracellular matrix proteome

The extracellular matrix is a vital compartment in plants with a prominent role in defence against pathogen attack. Using a maize cell suspension culture system and pathogen elicitors, responses to pathogen attack that are localised to the extracellular matrix were examined by a proteomic approach. Elicitor treatment of cell cultures induced a rapid change in the phosphorylation status of extracellular peroxidases, the apparent disappearance of a putative extracellular beta-N-acetylglucosamonidase, and accumulation of a secreted putative xylanase inhibitor protein. Onset of the defence response was attended by an accumulation of glyceraldehyde-3-phosphate dehydrogenase and a fragment of a putative heat shock protein. Several distinct spots of both proteins, which preferentially accumulated in cell wall protein fractions, were identified. These three novel observations, viz. (i) secretion of a new class of putative enzyme inhibitor, (ii) the apparent recruitment of classical cytosolic proteins into the cell wall and (ii) the change in phosphorylation status of extracellular matrix proteins, suggest that the extracellular matrix plays a complex role in defence. We discuss the role of the extracellular matrix in signal modulation during pathogen-induced defence responses.