PV技术的计算机处理及其在树木水分关系研究中的应用

ＰＶ技术的计算机处理及其在树木水分关系研究中的应用刘建伟,刘雅荣,王世绩（中国林业科学研究院林业研究所,北京１０００９１）ＣｏｍｐｕｔｅｒＰｒｏｃｅｓｓｉｎｇｏｆＰＶＣｕｒｖｅＡｎａｌｙｓｉｓａｎｄＩｔｓＡｐｐｌｉｃａｔｉｏｎｉｎｔｈｅＲｅｓｅａｒｃ...     

解离增强镧系元素荧光免疫分析灵敏度的改进

为提高解离增强镧系荧光免疫分析(DELFIA)的灵敏度或信／噪比,进行了一些重要的方法学研究．观察到了对于不同的铕量,荧光响应和信／噪比都随着增强液体积而明显地变化．对于确定的铕量,存在一个最佳体积,且铕量越小,其最佳体积也越小．实验中选择最佳体积是重要的．研究了增强液制备技术,发展了微滴定板条有效的清洗和干燥方法,使本底荧光明显降低．     

触珠蛋白研究进展

触珠蛋白是一种酸性糖蛋白,属急性期反应蛋白之一,由于所含轻链类型的不同,触珠蛋白具有遗传多态性,触珠蛋白的合成和降解主要在肝脏进行,并受细胞因子、前列腺素、激素等的调节,触珠蛋白具有广泛的生物学功能,可能是一种重要的调节蛋白.     

鲑鱼生长激素基因分泌型表达质粒的构建

生长激素(GH)是动物垂体前叶分泌的一种多肽类激素.应用分子重组及PCR等技术,构建了一种鲑鱼生长激素基因分泌型表达质粒pOsGH153,使编码鲑鱼生长激素成熟肽的序列克隆在大肠杆菌分泌型表达载体PIN-Ⅲ-ompA内,直接位于编码大肠杆菌外膜蛋白A信号肽序列的下游,在Lpp-Lac杂合启动子控制下,经IPTG诱导,分子量约23 000的鲑鱼生长激素在大肠杆菌中获得高效表达,该产物具有天然鲑鱼生长激素的免疫活性,直接分泌到细胞周质,而信号肽被自动剪除.     

自然贮存苹果的生化分析及形态解剖学研究

本文对自然贮存六个月的秦冠、红富士及新红星苹果果实在生化成分及显微、超微结构上进行了对比研究,发现红富士果实中果胶质、总糖及粗蛋白含量均高于其它品种;三种果实表面均有不同形态的蜡质分布;果皮角化层以红富士较厚,新红星次之;不同品种果肉细胞的形态有明显差异。本文对此结果进行了初步的探讨,以期为苹果贮存研究积累资料。     

民勤红砂岗地区绵刺分布和繁殖方式及濒危原因调查

本文调查了民勤红砂岗地区绵刺的分布现状和面积,初步观测了它的生活习性和繁殖方式,发现枝条生根是它的重要繁殖方式,并对绵刺濒危的原因和保护措施进行了讨论。     

合理用药生态控制茶小绿叶蝉主要措施与评价

合理用药生态控制茶小绿叶蝉主要措施与评价张觉晚,王沅江（湖南省茶叶研究所,长沙４１０１４５）ＰｒｉｎｃｉｐａｌＴｅｃｈｎｉｑｕｅｓｏｆＲａｔｉｏｎａｌＵｓｅｏｆＰｅｓｔｉｃｉｄｅｆｏｒＥｃｏｌｏｇｉｃａｌＣｏｎｔｒｏｌｏｖｅｒＴｅａＧｒｅｓｎＬｅａｆ...     

短杆菌肽通道内离子K~＋，Na~＋，Li~＋与水的相关性

基于右手螺旋短杆菌肽Ａ离子通道模型,利用分子动力学计算机模拟方法研究了通道内离子Ｋ＋,Ｎａ＋,Ｌｉ＋与水分子的相关性．     

Sucrose Synthase,Starch Accumulation,and Tomato Fruit Sink Strength

Contrasting evidence has accumulated regarding the role of acid invertase and sucrose synthase in tomato fruit sink establishment and maintenance. In this work the relationships among the activities of sucrose synthase and acid invertase, Lycopersicon esculentum Mill cv UC-82B fruit growth, and starch accumulation were analyzed in fruit at 0 to 39 d after anthesis. Sucrose synthase, but not acid invertase, was found to be positively correlated with tomato fruit relative growth rate and with starch content in the pericarp tissue. A similar association between sucrose synthase activity and starch accumulation was also evident in the basal portion of the stem. Heat-shock treatments, which inhibited the increase in sucrose synthase activity at the beginning of the light period and had no effect on acid invertase activity, were used to examine the importance of sucrose synthase in relation to sucrose metabolism and starch synthesis. After the heat-shock treatment, concomitantly with the suppressed sucrose synthase activity relative to the controls, there was a reduction in sucrose cleavage and starch accumulation. These data substantiate the conclusion that, during the early phases of tomato fruit development, sucrose synthase rather than acid invertase is the dominant enzyme in metabolizing imported sucrose, which in turn plays a part in regulating the import of sucrose into the fruit.