华南胡椒化学成分的研究

从胡椒科植物华南胡椒（Ｐｉｐｅｒ ａｕｓｔｒｏｓｉｎｅｎｓｅ Ｃ．ＤＣ．）中分离出七个成分,经光谱分析和理化常数测定,分别鉴定为Ｎ－ｉｓｏｂｕｔｙｌ－３（３＇,４＇－ｍｅｔｈｙｌｅｎｅｄｉｏｘｙ－５＇－ｍｅｔｈｏｘｙｐｈｅｎｙｌ）－２Ｅ－ｔｒｉｍｏｎｏｉｅｎａｍｉｄｅ（Ｉ）、Ｎ－ｉｓｏｂｕｔｙｌ－７（３＇,４＇－ｍｅｔｙｌｅｎｅｄｉｏｘｙｐｈｅｎｙｌ）－２Ｅ,４Ｅ－ｈｅｐｔａｄｉｅｎａｍｉｄｅ（Ⅱ     

病毒基因组启动子识别的人工神经网络方法

本文运用神经网络方法,并结合病毒基因分子生物学有关理论与统计事实,对病毒基因启动子区域进行了识别,文中选择了共35个基因组,作为研究对象．学习组选择了28个基因组,预测组选择了7个基因组,结果表明,将神经网络模型与病毒基因有关理论相结合,能够运用计算方法,以大量的可能启动子组合中排列出唯一的启动子区域．     

新的生物杀虫剂螨虫素对棉花、蔬菜害虫的毒力评价及应用技术研究

本文通过生物杀虫剂螨虫素对棉花、蔬菜害虫的毒力评价及田间应用研究表明,它对棉朱砂叶螨和菜青虫两种害虫杀伤力最强,其中LC90分别为0．078ppm和0．013ppm。兼有胃毒、触杀作用,残效期较长,分别达13天和9天。田间小区试验认为防治菜青虫和茄朱砂叶螨以2ppm浓度为宜。防治适期为卵孵化初期或低龄幼(若)虫（螨)期,其防效能达90%以上。该药特点用药量低、效果好、无公害、值得推广。     

生态蛇胆美容霜对治疗蠕形螨病及护肤美容的效果观察

本文报道了微生态制剂－生态蛇胆美容霜治疗蠕形螨病及其合并症的观察结果。用透明胶带定量计数法检查１１３１人,其中检出毛囊虫阳性者４０４例,定居率为３５．７％,检出毛囊出共４１８只。对４０４例毛囊虫阳性分两组,即实验组,和对照组进行对照。结果实验组２５１例中只有２例仍为阳性,其余转为阴性。     

湿地松粉蚧夏季数量凋落的原因分析

利用生命表技术及其相应的控制指数分析方法,对新侵入害虫湿地松粉蚧Ｏｒａｏｅｌｌａａ－ｃｕｔａ（Ｌｏｂｄｅｌｌ）夏季数量凋落的某些因子进行了量化分析。结果表明：在广东南部的新侵入区,夏季高温引起的松梢迅速老化,上代为害以后引起的营养质量的变化、拥挤以及煤污病的严重发生等,均对湿地松粉蚧夏季种群数量的凋落有着明显的影响,其总的排除作用控制指数ＥＩＰＣ为４６．８９,如果排除这几个因子的作用,下代种群的数量将为当代的４６．８９倍。     

Saturated Molecular Map of the Rice Genome Based on an Interspecific Backcross Population

A molecular map has been constructed for the rice genome comprised of 726 markers (mainly restriction fragment length polymorphisms; RFLPs). The mapping population was derived from a backcross between cultivated rice, Oryza sativa, and its wild African relative, Oryza longistaminata. The very high level of polymorphism between these species, combined with the use of polymerase chain reaction-amplified cDNA libraries, contributed to mapping efficiency. A subset of the probes used in this study was previously used to construct an RFLP map derived from an inter subspecific cross, providing a basis for comparison of the two maps and of the relative mapping efficiencies in the two crosses. In addition to the previously described PstI genomic rice library, three cDNA libraries from rice (Oryza), oat (Avena) and barley (Hordeum) were used in this mapping project. Levels of polymorphism detected by each and the frequency of identifying heterologous sequences for use in rice mapping are discussed. Though strong reproductive barriers isolate O. sativa from O. longistaminata, the percentage of markers showing distorted segregation in this backcross population was not significantly different than that observed in an intraspecific F(2) population previously used for mapping. The map contains 1491 cM with an average interval size of 4.0 cM on the framework map, and 2.0 cM overall. A total of 238 markers from the previously described PstI genomic rice library, 250 markers from a cDNA library of rice (Oryza), 112 cDNA markers from oat (Avena), and 20 cDNA markers from a barley (Hordeum) library, two genomic clones from maize (Zea), 11 microsatellite markers, three telomere markers, eleven isozymes, 26 cloned genes, six RAPD, and 47 mutant phenotypes were used in this mapping project. Applications of a molecular map for plant improvement are discussed.     

Analysis of genome-specific sequences inPhleum species: Identification and use for study of genomic relationships

Sau3AI shot gun cloning and colony hybridization with total genomic probes were used to isolate genome-specific sequences inPhleum species. The total DNA isolated from diploid speciesP. alpinum andP. bertolonii was partially digested withSau3AI and cloned using pUC19 as a vector to anE. coli strain DH5mcr. A partial genomic DNA library consisting of 3030 colonies for the genome ofP. alpinum and one consisting of 3240 colonies for the genome ofP. bertolonii were constructed. Twelve hundred and thirty colonies from the DNA library ofP. alpinum and 1320 from that ofP. bertolonii were respectively blotted to membrane filters and hybridized to the total genomic probes from these two species. Eight clones specific toP. alpinum and 13 specific toP. bertolonii were isolated through colony hybridization and further dot-blot hybridization. Most of these clones may carry highly or moderately repetitive sequences. Three sequences specific toP. alpinum and 3 specific toP. bertolonii were used as probes to hybridize theEcoRI-digested DNA samples from four species,P. alpinum,P. bertolonii,P. pratense andP. montanum, on Southern blot. The results from these hybridization experiments showed that all 3P. bertolonii-specific probes and 2 of the 3P. alpinum-specific probes hybridized to the DNA ofP. pratense, thus confirming the conclusion of the close relationships between the cultivated timothy and its two wild relatives that was drawn in our previous study using the C-banding technique.