全文获取类型
收费全文 | 31275篇 |
免费 | 2547篇 |
国内免费 | 2702篇 |
专业分类
36524篇 |
出版年
2024年 | 76篇 |
2023年 | 412篇 |
2022年 | 1078篇 |
2021年 | 1670篇 |
2020年 | 1177篇 |
2019年 | 1391篇 |
2018年 | 1353篇 |
2017年 | 999篇 |
2016年 | 1362篇 |
2015年 | 1872篇 |
2014年 | 2248篇 |
2013年 | 2466篇 |
2012年 | 2892篇 |
2011年 | 2473篇 |
2010年 | 1545篇 |
2009年 | 1369篇 |
2008年 | 1567篇 |
2007年 | 1398篇 |
2006年 | 1209篇 |
2005年 | 1064篇 |
2004年 | 937篇 |
2003年 | 858篇 |
2002年 | 724篇 |
2001年 | 632篇 |
2000年 | 518篇 |
1999年 | 489篇 |
1998年 | 322篇 |
1997年 | 312篇 |
1996年 | 306篇 |
1995年 | 282篇 |
1994年 | 255篇 |
1993年 | 164篇 |
1992年 | 222篇 |
1991年 | 166篇 |
1990年 | 141篇 |
1989年 | 122篇 |
1988年 | 81篇 |
1987年 | 105篇 |
1986年 | 60篇 |
1985年 | 59篇 |
1984年 | 47篇 |
1983年 | 32篇 |
1982年 | 34篇 |
1981年 | 20篇 |
1980年 | 8篇 |
1979年 | 6篇 |
1950年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
41.
Jiwen Liu Zice Fu An-Rong Li Michael Johnson Liusheng Zhu Andrew Marcus Jay Danao Tim Sullivan George Tonn Tassie Collins Julio Medina 《Bioorganic & medicinal chemistry letters》2009,19(17):5114-5118
The evaluation of the CXCR3 antagonist AMG 487 in clinic trials was complicated due to the formation of an active metabolite. In this Letter, we will discuss the further optimization of the quinazolinone series that led to the discovery of compounds devoid of the formation of the active metabolite that was seen with AMG 487. In addition, these compounds also feature increased potency and good pharmacokinetic properties. We will also discuss the efficacy of the lead compound 34 in a mouse model of cellular recruitment induced by bleomycin. 相似文献
42.
Mengjiao Zhu Lijie Han Ya Lv Yishan Liu Pan Li Haichun Jing Hongwei Cai 《植物学报(英文版)》2018,60(10):938-955
Tillering contributes to grain yield and plant architecture and therefore is an agronomically important trait in sorghum (Sorghum bicolor). Here, we identified and functionally characterized a mutant of the Non‐dormant Axillary Bud 1 (NAB1) gene from an ethyl methanesulfonate‐mutagenized sorghum population. The nab1 mutants have increased tillering and reduced plant height. Map‐based cloning revealed that NAB1 encodes a carotenoid‐cleavage dioxygenase 7 (CCD7) orthologous to rice (Oryza sativa) HIGH‐TILLERING DWARF1/DWARF17 and Arabidopsis thaliana MORE AXILLARY BRANCHING 3. NAB1 is primarily expressed in axillary nodes and tiller bases and NAB1 localizes to chloroplasts. The nab1 mutation causes outgrowth of basal axillary buds; removing these non‐dormant basal axillary buds restored the wild‐type phenotype. The tillering of nab1 plants was completely suppressed by exogenous application of the synthetic strigolactone analog GR24. Moreover, the nab1 plants had no detectable strigolactones and displayed stronger polar auxin transport than wild‐type plants. Finally, RNA‐seq showed that the expression of genes involved in multiple processes, including auxin‐related genes, was significantly altered in nab1. These results suggest that NAB1 functions in strigolactone biosynthesis and the regulation of shoot branching via an interaction with auxin transport. 相似文献
43.
从皖南尖吻蝮蛇(Agkistrodonacutus)毒液中经DEAE-Sepharose和SephacrylS-200两步凝胶柱层析首次纯化出一种中分子量出血毒素(简称AaHⅣ).经SDS-PAGE和等电聚焦凝胶电泳测定其分子量为44kD,等电点为pH5.0.从500mg粗毒中可获得20mgAaHⅣ纯品.AaHⅣ有较强的出血活性,最小出血剂量(MHD)为0.4μg. 相似文献
44.
45.
籼粳亚种间杂交稻米脂肪含量的遗传分析 总被引:4,自引:0,他引:4
用包括基因型×环境互作效应的种子性状遗传模型,研究了籼粳亚种间杂交稻米脂肪含量的遗传特性,结果表明:在籼粳杂种中,脂肪含量的遗传表达主要受控于种子直接加性效应和母体加性效应,以前者为主.基因型X环境互作主要表现为显性(包括直接显性和母体显性)X环境以及细胞质X环境工作.直接近传率和母体遗传率都极显著.此外,根据遗传效应预测值对供试条本的利用价值作了评价. 相似文献
46.
47.
48.
Redox-active iron mediates amyloid-beta toxicity 总被引:12,自引:0,他引:12
Rottkamp CA Raina AK Zhu X Gaier E Bush AI Atwood CS Chevion M Perry G Smith MA 《Free radical biology & medicine》2001,30(4):447-450
While amyloid-beta toxicity is mediated by oxidative stress and can be attenuated by antioxidants, the actual biochemical mechanism underlying neurotoxicity remains to be established. However, since aggregated amyloid-beta can interact with transition metals, such as iron, both in vitro and in vivo, we suspected that bound iron might be the mediator of toxicity such that holo- and apo-amyloid would have differential effects on cellular viability. Here we demonstrate that when amyloid-beta is pretreated with the iron chelator deferoxamine, neuronal toxicity is significantly attenuated while conversely, incubation of holo-amyloid-beta with excess free iron restores toxicity to original levels. These data, taken together with the known sequelae of amyloid-beta, suggest that the toxicity of amyloid-beta is mediated, at least in part, via redox-active iron that precipitates lipid peroxidation and cellular oxidative stress. 相似文献
49.
Xiang Chen 《Journal of biological physics》2013,39(4):607-624
A benzamide molecule is used as a “reader” molecule to form hydrogen bonds with five single DNA bases, i.e., four normal single DNA bases A,T,C,G and one for 5methylC. The whole molecule is then attached to the gold surface so that a meta-molecule junction is formed. We calculate the transmission function and conductance for the five metal–molecule systems, with the implementation of density functional theory-based non-equilibrium Green function method. Our results show that each DNA base exhibits a unique conductance and most of them are on the pS level. The distinguishable conductance of each DNA base provides a way for the fast sequencing of DNA. We also investigate the dependence of conductivity of such a metal–molecule system on the hydrogen bond length between the “reader” molecule and DNA base, which shows that conductance follows an exponential decay as the hydrogen bond length increases, i.e., the conductivity is highly sensitive to the change in hydrogen bond length. 相似文献
50.
Li W Tian H Li L Li S Yue W Chen Z Qi L Hu W Zhu Y Hao B Gao C Si L Gao F 《生物化学与生物物理学报(英文版)》2012,44(7):577-583
Lung cancer is the leading cause of cancer-related mortality all over the world. In recent years, pulmonary adenocarcinoma has surpassed squamous cell carcinoma in frequency and is the predominant form of lung cancer in many countries. Epidemiological investigations have shown an inverse relationship between garlic (Allium sativum) consumption and death rate from many cancers. Diallyl trisulfide (DATS) is one of the garlic-derived compounds (also known as: organosulfer compounds, OSC). DATS can induce apoptosis and inhibit the growth of many cancer cell lines. Our study demonstrated that the apoptotic incidents induced by DATS were a mitochondria-dependent caspase cascade through a significant decrease of the anti-apoptotic Bcl-2 that resulted in up-regulation of the ratio of Bax/Bcl-2 and the activity of caspase-3, -8, and -9. Eventually, DATS induced the apoptosis and inhibited the proliferation in a concentration- and time-dependent manner. Furthermore, by establishing an animal model of female BALB/c nude mice with A549 xenografts, we found that oral gavage of DATS significantly retarded growth of A549 xenografts in nude mice without causing weight loss or any other side effects compared with the control group. All the evidence both in vitro and in vivo suggested that DATS could be an ideal anti-cancer drug. 相似文献