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971.
Zhijing Xie Xin Sun Johannes Lux TingWen Chen Mikhail Potapov Donghui Wu Stefan Scheu 《Ecology and evolution》2022,12(2)
Altitudinal changes in the diversity of plants and animals have been well documented; however, soil animals received little attention in this context and it is unclear whether their diversity follows general altitudinal distribution patterns. Changbai Mountain is one of few well‐conserved mountain regions comprising natural ecosystems on the Eurasian continent. Here, we present a comprehensive analysis of the diversity and community composition of Collembola along ten altitudinal sites representing five vegetation types from forest to alpine tundra. Among 7834 Collembola individuals, 84 morphospecies were identified. Species richness varied marginally significant with altitude and generally followed a unimodal relationship with altitude. By contrast, the density of Collembola did not change in a consistent way with altitude. Collembola communities changed gradually with altitude, with local habitat‐related factors (soil and litter carbon‐to‐nitrogen ratio, litter carbon content, and soil pH) and climatic variables (precipitation seasonality) identified as major drivers of changes in Collembola community composition. Notably, local habitat‐related factors explained more variation in Collembola assemblages than climatic variables. The results suggest that local habitat‐related factors including precipitation and temperature are the main drivers of changes in Collembola communities with altitude. Specifically, soil and litter carbon‐to‐nitrogen ratio correlated positively with Collembola communities at high altitudes, whereas soil pH correlated positively at low altitudes. This documents that altitudinal gradients provide unique opportunities for identifying factors driving the community composition of not only above‐ but also belowground invertebrates. 相似文献
972.
973.
974.
Xinxin Wang Xin Ma Gaobo Wei Weirui Ma Zhen Zhang Xuepeng Chen Lei Gao Zhenbo Liu Yue Yuan Lizhi Yi Jun Wang Toshinobu Tokumoto Junjiu Huang Dahua Chen Jian Zhang Jiang Liu 《基因组蛋白质组与生物信息学报(英文版)》2021,19(1):48-63
DNA methylation is a prevalent epigenetic modification in vertebrates, and it has been shown to be involved the regulation of gene expression and embryo development. However, it remains unclear how DNA methylation regulates sexual development, especially in species without sex chromosomes. To determine this, we utilized zebrafish to investigate DNA methylation reprogramming during juvenile germ cell development and adult female-to-male sex transition.We reveal that primordial germ cells(PGCs) undergo significant DNA methylation reprogramming during germ cell development, and the methylome of PGCs is reset to an oocyte/ovary-like pattern at 9 days post fertilization(9 dpf). When DNA methyltransferase(DNMT) activity in juveniles was blocked after 9 dpf, the zebrafish developed into females. We also show that Tet3 is involved in PGC development. Notably, we find that DNA methylome reprogramming during adult zebrafish sex transition is similar to the reprogramming during the sex differentiation from 9 dpf PGCs to sperm. Furthermore, inhibiting DNMT activity can prevent the female-to-male sex transition, suggesting that methylation reprogramming is required for zebrafish sex transition. In summary, DNA methylation plays important roles in zebrafish germ cell development and sexual plasticity. 相似文献
975.
为获得辽东丁香(Syringa villosa subsp. wolfii)叶绿体全基因组的基本特征,采用高通量测序技术分析了其叶绿体基因组序列信息,并讨论其系统演化位置。结果表明:(1)辽东丁香叶绿体基因组全长156 517 bp,具有典型的四分体结构;具有131个功能基因,包括36个tRNA基因、8个rRNA基因和87个蛋白质编码基因。(2)该叶绿体基因组蛋白编码区的总密码子偏好性(RSCU)分析显示,RSCU值>1的密码子有31个,其中以A/U碱基结尾的有21个;RSCU值<1的密码子有34个,其中以G/C碱基结尾的密码子有22个。(3)在辽东丁香的叶绿体基因组中,检测出334个散在重复序列,包括170个正向重复序列和164个回文重复序列;检测到227个SSR位点,其中226个位点成功设计出PCR引物。(4)最大似然法构建系统进化树分析显示,辽东丁香与云南丁香(S. yunnanensis)亲缘关系最近。本研究通过对辽东丁香叶绿体基因组重复序列、IR边界、系统发育等进行分析,为辽东丁香后续的分子标记开发、系统发育分析、物种资源鉴定评价、DNA条形码开发等提供参考。 相似文献
976.
977.
Xin Li Chao Pan Zhicheng Liu Peng Sun Xiaoting Hua Erling Feng Yunsong Yu Jun Wu Li Zhu Hengliang Wang 《Microbial biotechnology》2022,15(2):703-716
Acinetobacter baumannii poses a serious threat to human health, mainly because of its widespread distribution and severe drug resistance. However, no licensed vaccines exist for this pathogen. In this study, we created a conjugate vaccine against A. baumannii by introducing an O-linked glycosylation system into the host strain. After demonstrating the ability of the vaccine to elicit Th1 and Th2 immune responses and observing its good safety in mouse a model, the strong in vitro bactericidal activity and prophylactic effects of the conjugate vaccine against infection were further demonstrated by evaluating post-infection tissue bacterial loads, observing suppressed serum pro-inflammatory cytokine levels. Additionally, the broad protection from the vaccine was further proved via lethal challenge with A. baumannii. Overall, these results indicated that the conjugate vaccine could elicit an efficient immune response and provide good protection against A. baumannii infection in murine sepsis models. Thus, the conjugate vaccine can be considered as a promising candidate vaccine for preventing A. baumannii infection. 相似文献
978.
979.
目的:了解F10基因在部分正常组织及肿瘤组织中的表达情况。方法:利用原位杂交和免疫组化方法对F10在部分正常组织和肿瘤组织中的mRNA和蛋白表达情况进行分析。结果:F10基因不仅在腺癌组织中表达呈阳性,在鳞癌组织中表现出较腺癌更强的强阳性,并且在正常组织中也有一定的表达。结论:F10是一个在多种组织普遍表达的细胞内蛋白,其功能可能与物质转运相关。 相似文献