Hedgehog-mediated paracrine interaction between hepatic stellate cells and marrow-derived mesenchymal stem cells

During liver injury, bone marrow-derived mesenchymal stem cells (MSCs) can migrate and differentiate into hepatocytes. Hepatic stellate cell (SC) activation is a pivotal event in the development of liver fibrosis. Therefore, we hypothesized that SCs may play an important role in regulating MSC proliferation and differentiation through the paracrine signaling pathway. We demonstrate that MSCs and SCs both express hedgehog (Hh) pathway components, including its ligands, receptors, and target genes. Transwell co-cultures of SCs and MSCs showed that the SCs produced sonic hedgehog (Shh), which enhanced the proliferation and differentiation of MSCs. These findings demonstrate that SCs indirectly modulate the activity of MSCs in vitro via the Hh pathway, and provide a plausible explanation for the mechanisms of transplanted MSCs in the treatment of liver fibrosis.     

遗传标记及其在作物品种鉴定中的应用

本文评述了用于作物品种鉴定的形态标记（morphological markers）、细胞标记（cytological markers）、生化标记（biochemical markers）、分子标记（molecular markers）的优缺点。重点评述了分子标记在作物品种鉴定中的应用。文中除对蛋白质电泳指纹图谱——同工酶和贮藏蛋白（包括醇溶性蛋白、清蛋白、谷蛋白、球蛋白等）电泳产生的指纹图谱的应用外,较详细地介绍了近年来DNA指纹图谱技术;包括限制片段长度多态性（restriction fragment length polymorphism,简称RFLP）、随机扩增多态性DNA (random amplified po lymorphic DNA,简称RAPD)、小卫星DNA（minisatellite DNA）、微卫星DNA（microsatellite DNA）,简单重复序列间扩增（intersimple sequence repeats,简称ISSR）,扩增片段长度多态性（amplified fragment length polymorphism,简称AFLP）以及CAPS (cleaved amplified polymorphic sequences)和SNPS (single nucleotide polymorphisms)对作物品种鉴定和新品种登记,品种纯度和真实性的检验以及品种间亲缘关系的探讨和在分类研究中的贡献等。     

传标记及其在作物品种鉴定中的应用

本文评述了用于作物品种鉴定的形态标记（morphological markers）、细胞标记（cytological markers）、生化标记（biochemical markers）、分子标记（molecular markers）的优缺点。重点评述了分子标记在作物品种鉴定中的应用。文中除对蛋白质电泳指纹图谱－－同工酶和贮茂蛋白（包括醇溶性蛋白、清蛋白、谷蛋白、球蛋白等）电泳产生的指纹图谱的应用外,较详细地介绍了近年来ＤＮＡ指纹图谱技术;包括限制片段长度多态性（restriction fragment length polymorphism,简称ＲＦＬＰ)、随机扩增多态性ＤＮＡ（random amplified polymorphic DNA,简称ＲＡＰＤ）、小卫星ＤＮＡ（minisatellite DNA）、微卫生ＤＡＮmicrosatellite DNA）,简单重复序列间扩增（intersimple sequence repeats,简称ＩＳＳＲ）,扩增片段长度多态性（amplified fragment length polymor-phism,简称ＡＦＬＰ）以及ＣＡＰＳ（cleaved amplified polymorphic sequences）和ＳＮＰＳ（single mucleotide polymor-phisms）对作物品种鉴定和新品种登记,品种纯度和真实性的检验以及品种间亲缘关系的探讨和在分类研究中的贡献等。     

广州市园林植物多样性及其效应的调查研究

选取广州市信园为研究个案,同时先取市内有代表性的5个生态园林区作对比进行植物多样性调查,计算物种多样性指数(SW指数).并把计算结果分别与园区内其它生物多样性的调查结果和环境指标(温度、湿度、土壤含水量、有机质质量分数、含氮量)的检测结果进行分析.结果表明:①有机质质量百分比随植物SW指数上升呈增高趋势, 良好的土壤环境又会促进植物多样性发展;②鸟类、其他小型动物的种类数随植物SW指数上升呈现增高趋势,即植物多样性决定动物物种丰富程度.建议广州市园区的SW指数不应低于2.5.     

小麦高分子量谷蛋白亚基对加工品质影响的效应分析

分析了 2 50份小麦材料的高分子量谷蛋白亚基 (HMW- GS)组成以及其中 66份材料的加工品质及面条制作品质。回归分析表明 :HMW- GS与 1 0种加工品质性状均有显著的线性关系。不同亚基对综合品质效应的得分大小依次为 :Glu- Al,1 >2 * >null;Glu- Bl,1 4 +1 5>7+8>1 7+1 8>>7+9;Glu- Dl,5+1 0 >>2 +1 2 >4+1 2。不同基因位点对品质的贡献大小顺序为 :Glu- Dl>Glu- Al>Glu- Bl。首次提出了 HMW- GS综合品质评分系统     

Stability ofAlternaria toxins in fruit juices and wine

Alternaria alternata is a common fungal parasite on fruits and other plants and produces a number of mycotoxins, including alternariol (3,7,9-trihydroxy-1-methyl-6H-dibenzo [b,d]pyran-6-one), alternariol monomethyl ether (3,7-dihydroxy-9-methoxy-1-methyl-6H-dibenzo[b,d]pyran-6-one), and the mutagen altertoxin I {[1S-(1α,12aβ,12bα)] 1,2,11,12,12a, 12b-hexahydro-1,4,9,12a-tetrahydroxy-3,10-perylenedione}. Alternariol and alternariol monomethyl ether have previously been detected in some samples of fruit beverages. Stability studies of these toxins as well as altertoxin I added to fruit juices and wine (10–100 ng/mL) were carried out. To include altertoxin I in the analysis, cleanup with a polymer-based Varian Abselut solid phase extraction column was used, as recoveries from C-18 columns were low. The stabilities of alternariol and alternariol monomethyl ether in a low acid apple juice containing no declared vitamin C were compared with those in the same juice containing added vitamin C (60 mg/175 ml); there were no apparent losses at room temperature over 20 days or at 80°C after 20 min. in either juice. Altertoxin I was moderately stable in pH 3 buffer (75% remaining after a two week period). Furthermore, altertoxin I was stable or moderately stable in three brands of apple juice tested over 1–27 day periods and in a sample of red grape juice over 7 days. It is concluded that altertoxin I is sufficiently stable to be found in fruit juices and should be included in methods for alternariol and alternariol monomethyl ether.     

认识和改良中国小麦蛋白质量的遗传基础:策略与现有的研究

认识和改良中国小麦蛋白质量的遗传基础:策略与现有的研究@王道文$中国科学院遗传研究所植物细胞与染色体工程国家重点实验室!北京100101@曲乐庆$中国科学院遗传研究所植物细胞与染色体工程国家重点实验室!北京100101@贾旭$中国科学院遗传研究所植物细胞与染色体工程国家重点实验室!北京100101@张相岐$中国科学院遗传研究所植物细胞与染色体工程国家重点实验室!北京100101@万永芳$中国科学院遗传研究所植物细胞与染色体工程国家重点实验室!北京100101@李振声$中国科学院遗传研究所植物细胞与染色体工程国家重点实验室!北京100101小麦;;蛋白…     

蓝尾石龙子的生长、两性异形及雌性繁殖

报道蓝尾石龙子（Eumeces elegans)的生长、两性异形和雌性繁殖,性成熟个体体色的两性差异显著,成年雄性体长、头长和头宽显著大于成年雌性,幼体体长生长率无显著的两性差异。成年雄体体长生长率显著大于成年雌体,因此,个体大小的两性异形是性成熟后发生的,体长小于50mm的幼体,头长和头宽无性差异;当体长大于50mm。雄性头长和头宽随体长的生长率显著大于雌性。并导致头部大小的两性异形,并随个体发育变得越来越显著,蓝尾石龙子产卵雌体的最小体长为69.3mm,大于此体长的雌体均年产单窝卵。窝卵数、窝卵重和平均卵重均与雌体体长呈正相关,平均值分别为6.4、2.783和0.554g。窝卵数与雌体产后状态无关,蓝尾石龙子雌体主要通过增加窝卵数和卵大小来增加繁殖输出。