Strategies for the computation of infrared CD and absorption spectra of biological molecules: ribonucleic acids.

We report observed and computed infrared (vibrational) circular dichroism spectra of a number of polyribonucleic acids in aqueous solutions in the 1600-1750 cm-1 spectral region, in which C = O and some nucleotide base ring stretching vibrations occur. The experimental data are compared with results calculated using different levels of sophistication within the exciton approach. We find that observed band shapes are generally well reproduced by these models, particularly if care is taken to determine the direction of the vibrational dipole transition moments accurately.     

Conformation and activity ofPhaseolus coccineus var. rubronanus lectin

The conformation of native and denaturedPhaseolus coccineus var. rubronanus lectin was studied by circular dichroism (CD) and correlated to the hemagglutinating activity. The far-UV CD spectrum at 25°C showed a broad, negative band around 223 nm and a positive one at 196 nm. CD data analysis of the lectin indicated a -sheet-rich protein. At high temperatures, the spectrum was blue-shifted with increasing magnitude; these changes correlated well with the loss of the activity. The conformation of lectin betweenpH 2 and 10 remained essentially unchanged. AtpH 13 the CD spectrum resembled that of unordered form with a negative band near 200 nm and the activity was completely lost. The denatured lectin in 6 M guanidine hydrochloride would be renatured upon diluting the denaturant to 0.75 M; the changes in CD spectrum again correlated well with the loss of the activity. The effect of sodium dodecyl sulfate on the lectin was drastic; it sharply increased thea-helix at the expense of the -sheet and reduced the activity; the changes reached a plateau above 20 mM surfactant.     

He—Ne激光对君子兰幼果和胡萝卜根的愈伤组织形成及生长的影响

利用6mv的He—Ne激光器,每天对君子兰幼果、胡萝卜根的外植体辐照5分钟,连续辐照20天,对愈伤组织的形成和生长有一定的促进作用。但同样条件下,辐照10分钟,对君子兰幼果,胡萝卜根的愈伤组织形成和生长都有明显的抑制作用。     

激光辐照真菌的酯酶,淀粉酶同工酶研究

本文报导了用He—Ne激光辐照真菌米曲酶的酯酶同工酶和淀粉同工酶的分析研究结果,实验表明激光对米曲霉同工酶有一定的影响。     

10CM短柱快速分离3-MH的实验研究

本研究在改进后短程序基础上,对氨基酸分离柱进行了改进。改进后的分离柱长为10cm。比原来20cm长柱分离3—MH的时间缩短了近1/2。实验所得的(回收率为97.59%,分离度0.89±0.02。变异系数1.17)这些指标较国外用其它方法所得的结果有良好的相关性。多次测定结果说明长柱与短柱比较无明显差异。证明了短柱对3—MH含量无影响。这一改进所建立的方法大大地缩短了样品的分析时间,节约了大量进口试剂,开展这方面的工作将有利益提高严重烧伤、创伤后蛋白质代谢和营养学等方面的研究水平。     

酵母多肽对几种细胞DNA合成的影响

<正> 我们用国内的新鲜酵母为材料,从中分离纯化到一种多肽——酵母多肽,其分子量为14kD。在低血清培养体系中能促使人成纤维细胞(HFB)和人脐静脉内皮细胞(HUVEC)的DNA合成。当培养液中酵母多肽的浓度为1μg/mL时能引起最大的刺激作用。但此多肽对胎牛心脏内皮细胞(FBHEC)的DNA合成则无作用。     

江苏海岸带陆生盐土植物矿质元素含量的特点及生物循环

陆生盐土植物在生长过程中吸收积累了大量的Cl和Na;从海向陆随着土壤和植被的生态演替,植物中Cl和Na的浓度逐渐降低;N与Cl、Na有相似的水平分布规律;植物种类是影响元素吸收积累的主要因素。在盐地碱蓬中N、P、K、Ca、Mg、Na、Cl、Mn和Zn的含量均是生长前期较高,随着其生长老化逐渐降低,大穗结缕草、白茅与盐地碱蓬相比,Ca的含量前期低后期高,Na、Mn、Cu和Zn的季节变化不明显。参加盐地碱蓬系统生物循环的元素中,Cl和Na的比例最大,在大穗结缕草和白茅生态系统中比例较小;由于白茅被收割利用,一些元素从此生态系统中流失。     

枯草杆菌BS224菌在防治Ⅲ°烧伤创面感染中痂下组织细菌数量的动态观察

对48例Ⅲ°烧伤病人的创面,定量植入枯草杆菌BS224菌后,分别在24h、48h、72h及96h做痂下组织细菌定量检测。结果显示:枯草杆菌对痂下组织的致病菌有明显的拮抗作用。感染创面的BS224菌体数量24—48小时显著增加,72—96小时而下降。与清洁创面的BS224菌动态变化上相同,呈常态曲线的规律变化。     

人巨细胞病毒对单纯疱疹病毒1型抗原表达的影响

我们用免疫胶体金色埋前标记技术和免疫荧光技术研究了人胚肺细胞(HEL)内,人巨细胞病毒(HCMV-AD_(169))对单纯疱疹病毒1型(HSV-ⅠSM_(44))抗原表达的影响,旨在探讨在细胞这一微生境内,一病毒对另一病毒可能发生的影响。电镜下计数HSV-1组和HCMV HSV-1组特异性结合金颗粒数得HSV-1组为657个,HCMV HSV-1组的总数为283个。t检验P<0.01,差别非常显著。并且HSV-1组细胞的胞浆中的病毒颗粒,比HCMV HSV-1组明显多。荧光显微镜下:HSV-1组阳性细胞数为689个HCMV HSV-1组只有484个,经poisson分布u检验,P<0.01,差别非常显著。免疫荧光实验还表明:HSV-1组,抗血清在1:320时仍有荧光清晰的阳性细胞,而HCMV HSV-1组,抗血清在1:160时,却无荧光阳性细胞。细胞病变效应(CPE)动态观察显示:HSV-1组8小时即有细胞病变,24小时蔓延整个单层;而HCMV HSV-1组超感染14小时才有细胞病变。24小时约有75％细胞受累。结果表明HCMV对HSV-1的抗原表达有明显的抑制作用。对抑制作用的可能机理及其在分子生态学中的意义,进行了讨论。