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131.
Zhen Wang Xinting Liu Jie Wang Zepeng Fan Jiayu Shen Yuanxing Chen Qingsong Shao Ying Zheng 《化学与生物多样性》2023,20(2):e202200773
Bletilla striata (Thunb.) Reichb.f. is a perennial herb with abundant active ingredients. Previous research mainly focused on its tubers, however, the study on flowers, especially the variation of active ingredient contents at different flowering stages, was rarely seen. This study analyzed the total phenols, flavonoids, polysaccharides, anthocyanins, and cyanidin-3-O-glucoside content of B. striata flowers which were in cultivated in Herb Garden of Zhejiang A&F University and collected in May, 2019, in order to investigate the changes in active ingredients and antioxidant capacity among different flowering stages (bud, initial, and full bloom). Changes in radical scavenging capability of DPPH (1,1-Diphenyl-2-picrylhydrazyl radical), ABTS (2,2′-azinobis(3-ethylbenzthiazoline-6-sulphonate)), and hydroxy were analyzed. Significant differences in active ingredient content of flowers were detected among different flowering stages. The total phenolic content increased continuously during the entire flowering stage. The contents of total flavonoid, total polysaccharide, and cyanidin-3-O-glucoside reached peaks at the initial blooming stage and then fell as the flowering process continued. The antioxidant activity in initial stage was the highest than in any other flowering stages. Therefore, we conclude that the initial blooming stage is the best harvesting stage of B. striata flowers. This study provides a robust basis for the harvest and utilization of B. striata flowers in food, medical, and cosmetic industries. 相似文献
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134.
以质粒pRK404为载体亚克隆含大豆根瘤菌吸氢酶结构基因(hup)的片段,构建成嵌合质粒pHR11、pHR4和pHR10。通过三亲本杂交将这些嵌合质粒导入无吸氢活性的Rhodobactersphaeroides241菌株(Hup-),均获得Hup+的接合子。利用启动子检测质粒pMP220证明,在hup对结构基因上游1.2kb内存在hup启动基因片段。以pRK2013为助质粒可将pHR11导入Enterobactercloacae和Klebsiellaoxytoca等土壤固氮菌株。本文以接合子E.cloacaeEH1113为例,通过对基因组DNASouthern杂交分析证明,嵌合质粒pHR11在EH1113中稳定贮存和复制。H2诱导接合子EH1113吸氢酶活性高表达,吸氢活性与放氢活性比值约为对照的两倍。当以延胡索酸为电子受体时,吸氢酶的吸氢作用支持菌株固氮酶活性的提高。 相似文献
135.
紫米基因与RFLP标记的连锁分析 总被引:12,自引:0,他引:12
选用种皮呈紫黑色的水稻体细胞无性系变异体黑珍米和其种皮呈无色的原始亲本Basmati370配制组合,同时应用121个DNA探针检测了黑珍米与Basmati370之间的RFLP。应用F2和F3群体研究了紫色种皮的遗传控制。结果表明,有一个显性主效基因控制着黑珍米和Basmati370在种皮颜色上的差异。通过多态性DNA探针与种皮颜色的共分离分析,发现该基因与水稻第四染色体上的DNA标记RG329和RG214连锁,与RG329和RG214的遗传图距分别为18.9cM和26.3cM。 相似文献
136.
DISCOVERY OF VERTEBRATE FOSSILS AND PALEOLITHIC ARTIFACTS IN DANJIANG SUBMERGING AREA AND ITS IMPLICATIONS 总被引:1,自引:1,他引:0
IntheareatobefloodedinthesecondengineeringstagefortheDanjiangreservoir,wediscovered16vertebratefossillocalitiesand52Paleolithicsitesin1994,andcollected603artifactsandmanyfossils.Ofthel6new1yfoundvertebratefossillocalities,threearereptilesitesrepresentedbydinosaureggsandlimbbones,andl3producemamma1s,including4Pale0gene,2Ne0geneand7Quaternarysites'ThemammalianlocalitiesareofPale0cene,Eocene,EarlyMiocene,andPli0ceneorEarlyPleistocene,MiddleandIntePleistocene,respectively.Theyfillinsomestrat… 相似文献
137.
A mutant of Rhodobacter capsulatus was identified in which an operon encoding a binding-protein-dependent transporter was interrupted by Tn5 transposition. Cloning and sequence analysis of the wild-type operon revealed a four-gene cluster with similarities to genes encoding periplasmic binding proteins (BztA), integral membrane proteins (BztB and BztC), and ATP-binding proteins (BztD). To assess the function of this putative binding-protein-dependent transport system, a mutant was constructed in which most of the bztABCD operon was deleted and replaced by an antibiotic-resistance marker. The deletion mutant grew more slowly than the wild type in NH-free medium supplemented by glutamate, glutamine, aspartate or asparagine; it was resistant to toxic analogues of Glu, Asp, and Asn at concentrations that inhibited growth of the wild type; and it was defective in the uptake of Glu, Gin, and Asp. A complementing plasmid containing the wild-type copy of bztABCD was able to rescue all the mutant phenotypes. Taken together, these results indicate that the proteins encoded by bztABCD are active in the uptake of Glu, Gin, Asp, and Asn. In addition, competition experiments, in which the ability of each of the four amino acids to compete for the transport of one another was examined, demonstrated that all four substrates share at least one component of this transport system. 相似文献
138.
Esculentoside A (EsA) is a saponin isolated from the roots of Phytolacca esculenta. Previous experiments have shown that it has strong anti-inflammatory effects. Tumour necrosis factor (TNF) is a very important inflammatory mediator. It is known that there are two types of TNF-TNFalpha is from macrophages/monocytes and TNFbeta is from activated lymphocytes. In order to study the mechanism of the anti-inflammatory effect of EsA, it was determined whether TNFalpha production from human peripheral monocytes was altered by EsA under lipopolysaccharide (LPS)-stimulated conditions. EsA was found to decrease TNFalpha production in a dose-dependent manner at concentrations higher than 1 mumol/l EsA. Recent studies have shown that EsA has a curative effect on chocolate cyst and other inflammatory diseases. Our previous studies have shown that EsA could reduce the release of platelet activating factor (PAF) from rat macrophages, and inhibit interleukin-1 and interleukin-6 production from routine macrophages. The reducing effects of EsA on the release of TNFalpha, IL-1, IL-6 and PAF may explain its anti-inflammatory effect. 相似文献
139.
离子导入法和渗透促进剂并用对裸鼠皮肤角质层影响的ESR研究 总被引:8,自引:0,他引:8
通过测定5-唑烷氮氧自由基硬脂酸(5-DSA)标记裸鼠皮肤角质层的ESR谱,研究离子导入法与渗透促进剂100%月桂氮酮(100%AZ)、5%月桂氨 酮/丙二醇溶液(5% AZ/PG)和10%油酸/丙二醇溶液(10%OA/PG)并用对裸鼠皮肤角质层的影响。离子导入法处理皮肤后,标记物序参数降低,各向同性超精细分裂偶合常数增大,表明低密度电流能够引起皮肤角质层细胞间脂质排列有序性降低,流动性增大,极性增大;离子导入法与渗透促进剂并用处理皮肤后,序参数进一步降低,各向同性超精细分裂偶合常数进一步增大,表明二者对皮肤角质层的影响具有协同作用。 相似文献
140.