NSA－Ⅱ型生理信号处理系统

介绍一种ＩＢＭ－ＰＣ系列微机生理信号采集、处理系统。该系统信号采集卡集程控放大、脉冲甄别、Ａ／Ｄ、Ｄ／Ａ等电路于一体,可满足各种生理信号的采集需要。采集信号时可长时间显示,动态观察。采样后,按需要给出各种图表与结果。部分处理方法采用独特数学模型,使结果更加精确。     

尼群地平对实验性糖尿病大鼠心肌收缩性的影响

腹腔注射链脲佐霉素（６５ｍｇ／ｋｇ）诱发Ｗｉｓｔａｒ大鼠糖尿病。糖尿病发病４周后,向饲料中加尼群地平（３０ｍｇ·ｋｇ－１·ｄ－１）。结果表明,糖尿病４周时大鼠心室舒张功能首先受损,８周后心室舒张和收缩功能均明显受累。尼群地平处理对糖尿病大鼠的心肌收缩性有一定的改善作用。提示尼群地平对大鼠糖尿病性心肌病有一定有益作用。     

藻－菌生态系统代谢功能的生态学研究

在室内模拟条件下,研究了一些生态因子对藻－菌（Ａ＋Ｂ）生态系统代谢有机碳（Ｃ６Ｈ１２Ｏ６）、ＮＨ３－Ｎ和无机磷（ＩＰ）的影响．研究结果表明,当藻－菌生态系统中藻（Ａ）或菌（Ｂ）的起始数量一定时,其代谢Ｃ６Ｈ１２Ｏ６的速率,随与之组合的Ｂ或Ａ的起始数量增加（数量比则相应降低）而增加．在光照和黑暗条件下,Ａ＋Ｂ系统代谢上述３种营养物质的速率均有一定的差异．黑暗下Ｃ６Ｈ１２Ｏ６的平均代谢速率较光照下高１２．３％（Ｐ＜０．０５）,ＩＰ和ＮＨ３－Ｎ的平均代谢速率则分别较光照下低１４．４％（Ｐ＜０．０５）和１６．２％（Ｐ＜０．００１）．在Ａ＋Ｂ系统和Ａ、Ｂ单培养物中,３种营养物质的代谢速率均随有机负荷量增加而增加,而且Ａ＋Ｂ系统的代谢速率分别高于单培养的Ａ和Ｂ,其中ＮＨ３－Ｎ代谢尤为显著．文章还就生态系统结构与功能的关系问题进行了讨论．     

利用马尾松年轮资料重建江苏暖湿地区气候的初步研究

利用树木年轮气候学方法,根据马尾松生长对气候变量具非线性响应,尝试重建江苏暖湿地区过去１１６年（１８７４～１９９０）的年平均气温和降水量。温度的重建效果优于降水量,实测值与重建值两者的缩减误差（ＲＥ）分别为０．１２和０．１０,两者的平均偏差百分率分别为２．３％和２０％。绝大部分重建结果可从观测记录和历史文献中得到验证,但在温度较高或降水量较多的若干年份,实测值与重建值有较大偏差。     

草鱼出血病病毒多肽的荧光染色

将草鱼出血病病毒（ＧｒａｓｓＣａｒｐＨｅｍｏｒｒｈａｇｅＶｉｒｕｓ,ＧＣＨＶ）置于还原性的溶液中,然后加入等体积的ＮａＨＣＯ３配制的异硫氰酸荧光索溶液进行多肽的标记,再经ＳＤＳ－ＰＡＧＥ分析,在紫外灯下即可检测到ＧＣＨＶ全部的１１个结构多肽的荧光带。该方法最小检测量为５００ｎｇ,由该方法回收的多肽具有抗原活性,可作为抗原进行免疫学实验。     

穴位激光照射对兔子宫类固醇激素受体的影响

对兔用Ｈｅ－Ｎｅ激光照射穴位,照射外生殖器,用类固醇激素受体测定法测定子宫雌二醇受体（ＥＲ）和孕酮受体（ＰＲ）含量。照射穴位后部分实验组的ＥＲ和ＰＲ浓度上升,与正常对照组相比较有显著或极显著差别（Ｐ＜０．０５,Ｐ＜０．０１）,ＥＲ提高１．７－３．２倍,ＰＲ提高２．７－３．７倍。实验结果提示激光可通过类固醇激素受体浓度的提高,在受体水平上影响机体代谢。     

Cumulus cells secrete a meiosi-inducing substance by stimulation with forskolin and dibutyric cyclic adenosine monophosphate

The role of the cumulus cells in initiating the resumption of meiosis after exposure to forskolin and dbcAMP was studied in the mouse. The resumption of meiosis was monitored by the percentage of germinal vesicle breakdown (GVBD) and polar body formation (PB). The cumulus-enclosed oocytes (CEO) and denuded oocytes (DO) were cultured with and without hypoxanthine (HX) in the culture medium. Three types of experiments were performed: (1) Effect of forskolin on spontaneous resumption of meiosis, i.e. cultures without HX, and two experiments in which HX is present throughout the culture: (2) Effect of transient exposure to forskolin or dibutyric-cyclic adenosinemonophosphate (dbcAMP) on GVBD prior to continued culture without forskolin or dbcAMP (oocyte priming). (3) Priming of CEO with forskolin for 2 hr, separation of cumulus cells and oocytes, followed by coculture of rejoined cumulus cells and oocytes, or coculture of the cumulus cells and new, unprimed DO. (1) Forskolin inhibited a spontaneous resumption of meiosis in a dose-dependent manner during the first 5 hr of culturing. After 22 hr all controls and CEO resumed meiosis, whereas only half of the DO did. (2) At least 1 hr of priming the CEO with forskolin is needed to induce GVBD and PB formation, but forskolin inhibited the resumption of meiosis when present for 24 hr. Similar results were obtained with a high concentration of dbcAMP. (3) A separation and rejoining of oocytes and cumulus cells after priming induced the resumption of meiosis in a significantly greater number of oocytes than in the control oocytes which were not primed. The GVBD of unstimulated DO also increased significantly when cocultured with cumulus cells from primed CEO. The percentage of GVBD in unprimed DO and in DO isolated from primed CEO was the same. We suggest that within 1–2 hr, forskolin and cAMP stimulate cumulus cells to produce a diffusible meiosis-inducing substance which overcomes HX-inhibition and induces oocyte maturation, including both GVBD and PB formation. The CEO must be primed for more than 2 hr before the resumption of meiosis in DO isolated from such CEO is induced. Oocyte-cumulus connections are crucial as far as initiating the production of a meiosis-inducing substance is concerned. Oocyte-cumulus connections are not needed for transferring this substance to the oocyte. © 1994 Wiley-Liss, Inc.     

缢蛏碱性磷酸酶胍溶液中分子折叠与活力变化研究

报道了缢蛏碱性磷酸酶（简称ＡＬＰ）经不同浓度盐酸胍处理时酶的分子构象所发生的变化以及酶变化和失活的动力学过程。在胍中酶荧光发射峰强度下降,紫外差光谱在２４６ｎｍ和２８５ｎｍ处出现２个负峰,ＣＤ谱中酶的α螺旋度下降,且随浓度增大,变化程度也加大。动力学研究表明,酶在０．５ｍｏｌ／Ｌ、１．０ｍｏｌ／Ｌ、２．０ｍｏｌ／Ｌ３．０ｍｏｌ／Ｌ、４．０ｍｏｌ／Ｌ盐酸胍中的变性速度常数分别为３．２１×１０~（－４）ｓ~（－１）、６．３８×１０~（－４）ｓ~（－１）、２．１７×１０~（－３）ｓ~（－１）、２．３３×１０~（－３）ｓ~９－１）、５．１７×１０~（－３）ｓ~（－１）;而酶在相应盐酸胍中的失活速度常数分别为２．３３×１０~（－４）ｓ~（－１）、３．５７×１０~（－４）ｓ~（－１）、５．８６×１０~（－４）ｓ~（－１）、１．１４×１０~（－３）ｓ~（－１）、３．４５×１０~（－３）ｓ~（－１）;表现为失活与构象伸展变化基本平行。     

Increase in cytoskeletal actin induced by inositol 1,4-bisphosphate in saponin-permeated pig platelets

Inositol 1,4-bisphosphate (IP₂), which rapidly accumulates during cell activation, strongly stimulates an increase in cytoskeletal actin in saponin-permeated platelets, and the effect is insensitive to 5′-Chloro-5′-deoxyadenosine. Within 10 s, the amount of cytoskeletal actin in platelets rapidly increases by 41%, and then slowly increases further. IP₂ induces the increase in cytoskeletal actin in a dose-dependent manner. The half-maximal effect requires approximately 2 μM of IP₂ Inositol 1,4,5- triphosphate, the messenger for Ca²⁺ release, causes the increase in cytoskeletal actin, but is less effective than IP₂. Inositol 1-monophosphate and inositol 2-monophosphate have no effect on cytoskeletal actin. Phorbol 12-myristate 13-acetate, which has been shown to activate IP₃ 5′-phosphatase through protein kinase C, stimulates the increase in cytoskeletal actin. Spermine, an inhibitor of IP₃ 5′-phosphatase, inhibits the thrombin stimulated increase in cytoskeletal actin. These results suggest that IP₂ may be a messenger that controls the organization of actin filaments during cell activation. This study presents the first evidence for IP₂ as a messenger during cell activation.