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91.
A headspace solid‐phase microextraction (HS‐SPME) method combined with gas chromatography‐mass spectrometry (GC/MS) was developed and optimized for the extraction and the analysis of volatile organic compounds (VOCs) from lavandin and fine lavender roots. Optimal parameters to extract volatile molecules from ground and intact roots were determined using a divinylbenzene‐carboxen‐polydimethylsiloxane (DVB/CAR/PDMS) coating fiber at 70 °C for 60 min. A total of 99 VOCs, including 40 monoterpenoids, 15 sesquiterpenoids, 1 diterpenoid and 2 coumarins were detected. The main compounds detected in lavandin roots were fenchol, borneol, and coumarin. Performances of the optimized SPME GC/MS method were evaluated via the comparison of VOC emissions between roots from different cultivars of fine lavender (7713 and maillette) and lavandin (abrial and grosso). Chemometric analysis, using partial least squares‐discriminant analysis (PLS‐DA), suggests fifteen significant features as potential discriminatory compounds. Among them, β‐phellandrene allows discrimination between lavender and lavandin varieties. 相似文献
92.
Traditional Mediterranean diet includes the halophyte Crithmum maritimum L. (Apiaceae) which can be found in the coastline of the Balearic Islands but also inland. Both areas differed in the environmental conditions, mainly in salinity which can affect the oxidative status of this species. The aim was to evaluate the antioxidant enzyme activities, polyphenols and the lipid peroxidation in leaves of wild C. maritimum growing in a natural coastal area influenced by marine salinity and an inland area without marine influence. The activities of the antioxidant enzymes catalase, superoxide dismutase and glutathione peroxidase as well as polyphenol and reduced glutathione content were significantly higher in the samples from coastline population, whereas no significant differences were found in glutathione reductase activity and in malondialdehyde levels. The production of H2O2 was also significantly higher in the population from coastline. In conclusion, C. maritimum adapt their antioxidant defense machinery to the different salinity conditions, avoiding the instauration of oxidative stress. 相似文献
93.
Regime shifts of Mediterranean forest carbon uptake and reduced resilience driven by multidecadal ocean surface temperatures 总被引:1,自引:0,他引:1
Jofre Carnicer Cristina Domingo‐Marimon Miquel Ninyerola Jesus Julio Camarero Ana Bastos Jorge Lpez‐Parages Laura Blanquer Beln Rodríguez‐Fonseca Timothy M. Lenton Vasilis Dakos Montserrat Ribas Emilia Gutirrez Josep Peuelas Xavier Pons 《Global Change Biology》2019,25(8):2825-2840
The mechanisms translating global circulation changes into rapid abrupt shifts in forest carbon capture in semi‐arid biomes remain poorly understood. Here, we report unprecedented multidecadal shifts in forest carbon uptake in semi‐arid Mediterranean pine forests in Spain over 1950–2012. The averaged carbon sink reduction varies between 31% and 37%, and reaches values in the range of 50% in the most affected forest stands. Regime shifts in forest carbon uptake are associated with climatic early warning signals, decreased forest regional synchrony and reduced long‐term carbon sink resilience. We identify the mechanisms linked to ocean multidecadal variability that shape regime shifts in carbon capture. First, we show that low‐frequency variations of the surface temperature of the Atlantic Ocean induce shifts in the non‐stationary effects of El Niño Southern Oscillation (ENSO) on regional forest carbon capture. Modelling evidence supports that the non‐stationary effects of ENSO can be propagated from tropical areas to semi‐arid Mediterranean biomes through atmospheric wave trains. Second, decadal changes in the Atlantic Multidecadal Oscillation (AMO) significantly alter sea–air heat exchanges, modifying in turn ocean vapour transport over land and land surface temperatures, and promoting sustained drought conditions in spring and summer that reduce forest carbon uptake. Third, we show that lagged effects of AMO on the winter North Atlantic Oscillation also contribute to the maintenance of long‐term droughts. Finally, we show that the reported strong, negative effects of ocean surface temperature (AMO) on forest carbon uptake in the last decades are unprecedented over the last 150 years. Our results provide new, unreported explanations for carbon uptake shifts in these drought‐prone forests and review the expected impacts of global warming on the profiled mechanisms. 相似文献
94.
MyROOT: a method and software for the semiautomatic measurement of primary root length in Arabidopsis seedlings 总被引:1,自引:0,他引:1
Isabel Betegn‐Putze Alejandro Gonzlez Xavier Sevillano David Blasco‐Escmez Ana I. Cao‐Delgado 《The Plant journal : for cell and molecular biology》2019,98(6):1145-1156
Root analysis is essential for both academic and agricultural research. Despite the great advances in root phenotyping and imaging, calculating root length is still performed manually and involves considerable amounts of labor and time. To overcome these limitations, we developed MyROOT, a software for the semiautomatic quantification of root growth of seedlings growing directly on agar plates. Our method automatically determines the scale from the image of the plate, and subsequently measures the root length of the individual plants. To this aim, MyROOT combines a bottom‐up root tracking approach with a hypocotyl detection algorithm. At the same time as providing accurate root measurements, MyROOT also significantly minimizes the user intervention required during the process. Using Arabidopsis, we tested MyROOT with seedlings from different growth stages and experimental conditions. When comparing the data obtained from this software with that of manual root measurements, we found a high correlation between both methods (R2 = 0.997). When compared with previous developed software with similar features (BRAT and EZ‐Rhizo), MyROOT offered an improved accuracy for root length measurements. Therefore, MyROOT will be of great use to the plant science community by permitting high‐throughput root length measurements while saving both labor and time. 相似文献
95.
Susana Toboso‐Chavero Ana Nadal Anna Petit‐Boix Oriol Pons Gara Villalba Xavier Gabarrell Alejandro Josa Joan Rieradevall 《Journal of Industrial Ecology》2019,23(4):767-780
Cities are rapidly growing and need to look for ways to optimize resource consumption. Metropolises are especially vulnerable in three main systems, often referred to as the FEW (i.e., food, energy, and water) nexus. In this context, urban rooftops are underutilized areas that might be used for the production of these resources. We developed the Roof Mosaic approach, which combines life cycle assessment with two rooftop guidelines, to analyze the technical feasibility and environmental implications of producing food and energy, and harvesting rainwater on rooftops through different combinations at different scales. To illustrate, we apply the Roof Mosaic approach to a densely populated neighborhood in a Mediterranean city. The building‐scale results show that integrating rainwater harvesting and food production would avoid relatively insignificant emissions (13.9–18.6 kg CO2 eq/inhabitant/year) in the use stage, but their construction would have low environmental impacts. In contrast, the application of energy systems (photovoltaic or solar thermal systems) combined with rainwater harvesting could potentially avoid higher CO2 eq emissions (177–196 kg CO2 eq/inhabitant/year) but generate higher environmental burdens in the construction phase. When applied at the neighborhood scale, the approach can be optimized to meet between 7% and 50% of FEW demands and avoid up to 157 tons CO2 eq/year. This approach is a useful guide to optimize the FEW nexus providing a range of options for the exploitation of rooftops at the local scale, which can aid cities in becoming self‐sufficient, optimizing resources, and reducing CO2 eq emissions. 相似文献
96.
Dodonov Pavel Harper Karen A. de Oliveira Xavier Rafael Silva Matos Dalva M. 《Plant Ecology》2019,220(7-8):741-756
Plant Ecology - Invasive grasses are an important threat in tropical savannas and grasslands and may be affected by natural and anthropogenic features of the environment. They may affect native... 相似文献
97.
Anthony Perrier Xavier Barlet David Rengel Philippe Prior Stéphane Poussier Stéphane Genin Alice Guidot 《Environmental microbiology》2019,21(8):3140-3152
An evolution experiment with the bacterial plant pathogen Ralstonia solanacearum revealed that several adaptive mutations conferring enhanced fitness in plants arose in the efpR gene encoding a regulator of virulence and metabolic functions. In this study, we found that an efpR mutant systematically displays colonies with two morphotypes: the type S (‘smooth’, similar to the wild type) and the type EV (‘efpR variant’). We demonstrated that the efpH gene, a homologue of efpR, plays a key role in the control of phenotypic heterogeneity, the ΔefpR-ΔefpH double mutant being stably locked into the EV type. Using mixed infection assays, we demonstrated that the type EV is metabolically more proficient than the type S and displays fitness gain in specific environments, whereas the type S has a better fitness into the plant environment. We provide evidence that this efpR-dependent phenotypic heterogeneity is a general feature of strains of the R. solanacearum species complex and could occur in natural conditions. This study highlights the potential role of phenotypic heterogeneity in this plant pathogen as an adaptive trait to changing environments. 相似文献
98.
Diego de Souza Gonalves Marina da Silva Ferreira Kamilla Xavier Gomes Claudia Rodríguez‐de La Noval Susie Coutinho Liedke Giovani Carlo Veríssimo da Costa Patricia Albuquerque Juliana Reis Cortines Regina Helena Saramago Peralta Jos Mauro Peralta Arturo Casadevall Allan J. Guimares 《Cellular microbiology》2019,21(10)
Free‐living amoebae (FLAs) are major reservoirs for a variety of bacteria, viruses, and fungi. The most studied mycophagic FLA, Acanthamoeba castellanii (Ac), is a potential environmental host for endemic fungal pathogens such as Cryptococcus spp., Histoplasma capsulatum, Blastomyces dermatitides, and Sporothrix schenckii. However, the mechanisms involved in this interaction are poorly understood. The aim of this work was to characterize the molecular instances that enable Ac to interact with and ingest fungal pathogens, a process that could lead to selection and maintenance of possible virulence factors. The interaction of Ac with a variety of fungal pathogens was analysed in a multifactorial evaluation that included the role of multiplicity of infection over time. Fungal binding to Ac surface by living image consisted of a quick process, and fungal initial extrusion (vomocytosis) was detected from 15 to 80 min depending on the organism. When these fungi were cocultured with the amoeba, only Candida albicans and Cryptococcus neoformans were able to grow, whereas Paracoccidioides brasiliensis and Sporothrix brasiliensis displayed unchanged viability. Yeasts of H. capsulatum and Saccharomyces cerevisiae were rapidly killed by Ac; however, some cells remained viable after 48 hr. To evaluate changes in fungal virulence upon cocultivation with Ac, recovered yeasts were used to infect Galleria mellonella, and in all instances, they killed the larvae faster than control yeasts. Surface biotinylated extracts of Ac exhibited intense fungal binding by FACS and fluorescence microscopy. Binding was also intense to mannose, and mass spectrometry identified Ac proteins with affinity to fungal surfaces including two putative transmembrane mannose‐binding proteins (MBP, L8WXW7 and MBP1, Q6J288). Consistent with interactions with such mannose‐binding proteins, Ac–fungi interactions were inhibited by mannose. These MBPs may be involved in fungal recognition by amoeba and promotes interactions that allow the emergence and maintenance of fungal virulence for animals. 相似文献
99.
Sez‐Jade Chen Nattawut Sinsuebphon Alena Rudkouskaya Margarida Barroso Xavier Intes Xavier Michalet 《Journal of biophotonics》2019,12(3)
We introduce a simple new approach for time‐resolved multiplexed analysis of complex systems using near‐infrared (NIR) dyes, applicable to in vitro and in vivo studies. We show that fast and precise in vitro quantification of NIR fluorophores' short (subnanosecond) lifetime and stoichiometry can be done using phasor analysis, a computationally efficient and user‐friendly representation of complex fluorescence intensity decays obtained with pulsed laser excitation and time‐gated camera imaging. We apply this approach to the study of binding equilibria by Förster resonant energy transfer using two different model systems: primary/secondary antibody binding in vitro and ligand/receptor binding in cell cultures. We then extend it to dynamic imaging of the pharmacokinetics of transferrin engagement with the transferrin receptor in live mice, elucidating the kinetics of differential transferrin accumulation in specific organs, straightforwardly differentiating specific from nonspecific binding. Our method, implemented in a freely‐available software, has the advantage of time‐resolved NIR imaging, including better tissue penetration and background‐free imaging, but simplifies and considerably speeds up data processing and interpretation, while remaining quantitative. These advances make this method attractive and of broad applicability for in vitro and in vivo molecular imaging and could be extended to applications as diverse as image‐guided surgery or optical tomography. 相似文献
100.
Capparicordis, genus novum is established for Capparicordis crotonoides, C. tweediana, and C. yunckeri, all new combinations here established for three former species of the New World Capparis, Sect. Colicodendron. The first two are xerophytic shrubs or small trees easily separated by flower color, and the last is a grapple-hook scrambler of which flowers are unknown. They have allopatric distributions: C. crotonoides is found in Peru and Ecuador west of the Andes, C. tweediana from Argentina to Bolivia and Paraguay east of the Andes, whereas C. yunckeri is a rare, local endemic from the arid woodlands near Coyoles in northern Honduras. All have stellate pubescence, broadly cordate to subrotund-reniform leaves with (sub)palmate venation at the leaf blade base; a valvate calyx with closed aestivation; baccate subspherical fruits dehiscent by 2–4 valves (indehiscent in C. yunckeri?); cochleate-reniform seeds surrounded by a pulp-derived sarcotesta densely infiltrated by unbranched, unicellular hairs from the testa; and snow-white embryos. Capparicordis crotonoides has n=8 chromosomes. 相似文献