全文获取类型
收费全文 | 170篇 |
免费 | 7篇 |
出版年
2024年 | 1篇 |
2023年 | 2篇 |
2022年 | 7篇 |
2021年 | 2篇 |
2020年 | 8篇 |
2019年 | 4篇 |
2018年 | 8篇 |
2017年 | 3篇 |
2016年 | 3篇 |
2015年 | 7篇 |
2014年 | 16篇 |
2013年 | 8篇 |
2012年 | 18篇 |
2011年 | 16篇 |
2010年 | 10篇 |
2009年 | 5篇 |
2008年 | 11篇 |
2007年 | 6篇 |
2006年 | 9篇 |
2005年 | 6篇 |
2004年 | 5篇 |
2003年 | 5篇 |
2002年 | 5篇 |
2000年 | 5篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1997年 | 2篇 |
1995年 | 1篇 |
1992年 | 1篇 |
1979年 | 1篇 |
排序方式: 共有177条查询结果,搜索用时 15 毫秒
31.
Enterovirus 2B viroporin has been involved in membrane permeabilization processes occurring late during cell infection. Even though 2B lacks an obvious signal sequence for translocation, the presence of a Lys-based amphipathic domain suggests that this product bears the intrinsic capacity for partitioning into negatively charged cytofacial membrane surfaces. Pore formation by poliovirus 2B attached to a maltose-binding protein (MBP) has been indeed demonstrated in pure lipid vesicles, a fact supporting spontaneous insertion into and direct permeabilization of membranes. Here, biochemical evidence is presented indicating that both processes are modulated by phosphatidylinositol and phosphatidylserine, the main anionic phospholipids existing in membranes of target organelles. Insertion into lipid monolayers and partitioning into phospholipid bilayers were sustained by both phospholipids. However, MBP-2B inserted into phosphatidylserine bilayers did not promote membrane permeabilization and addition of this lipid inhibited the leakage observed in phosphatidylinositol vesicles. Mathematical modelling of pore formation in membranes containing increasing phosphatidylserine percentages was consistent with its inhibitory effect arising from a higher reversibility of MBP-2B surface aggregation. These results support that 2B insertion and pore-opening are mechanistically distinguishable events modulated by the target membrane anionic phospholipids. 相似文献
32.
The influence of temperature (T) and initial gut contents onestimates of copepod gut clearance rate constant (GCRC) is reviewedfrom literature data. Previous results on the relationship betweentemperature and GCRC are confirmed, and the inclusion of initialgut contents did not increase the significance of the model.Nevertheless, a significant difference was found between theexperiments performed with pre-fed animals and animals fromthe environment. A high percentage of variability remains unexplained,but part of this is likely to be due to experimental error anddiffer ences in the methods used. 相似文献
33.
34.
Janire De-La-Torre Guillermo Quindós Cristina Marcos-Arias Xabier Marichalar-Mendia María Luisa Gainza Elena Eraso Amelia Acha-Sagredo José Manuel Aguirre-Urizar 《Revista iberoamericana de micología》2018,35(3):134-139
Background
Candida can be implicated in the pathology of chronic periodontitis.Aims
To analyze the oral Candida carriage in patients suffering from chronic periodontitis (CP) and its correlation with the severity of this condition.Methods
Microbiological samples were taken from 155 patients using the oral rinse (OR) technique and by using paper points in the periodontal pockets (GPP). These patients were divided into 3 groups: 89 patients without CP (control), 47 with moderate CP, and 19 with severe CP. Samples were cultured in a Candida chromogenic agar for Candida. Species were identified by microbiological and molecular methods.Results
Candida was isolated in the OR of 45 (50.6%), 21 (44.7%), and 11 (57.9%) patients, respectively, and in the GPP of 32 (36%), 14 (29.2%), and 10 (42.6%) patients from the control, moderate CP and severe CP groups, respectively. Candida was isolated more frequently and in a greater burden in OR than in GPP (p < 0.01). Candida albicans was the most prevalent species. GPP of patients with CP had poor fungal biodiversity (p < 0.01).Conclusions
Colonization by Candida was present in the samples of patients without CP, and with both moderate and severe CP. Nonetheless, patients with severe CP had a higher rate of Candida colonization, especially by C. albicans. 相似文献35.
Irigoien Xabier; Verheye Hans M.; Harris Roger P.; Harbour Derek 《Journal of plankton research》2005,27(8):735-742
We have analysed the daily egg production (EPR) and hatchingsuccess rates of the calanoid copepods Calanoides carinatusand Rhincalanus nasutus as a function of nano- and microplanktonconcentration and composition in the northern Benguela upwellingsystem off Namibia. Food concentration explained 55% (R. nasutus)to 62% (C. carinatus) of the EPR variability. We found no relationbetween the residuals of the food concentrationEPR regressionand the percentage of the different taxonomic components ofthe nano- and microplankton. Nor was there a relation with theproportion of the diatom Skeletonema costatum that dominatedthe major blooms or with the number of nano- and microplanktonspecies. We conclude that food quality differences could notbe attributed to the relative composition of microplanktonicparticles of the different groups (i.e. taxonomic composition). 相似文献
36.
37.
Pablo García-Díaz David S. L. Ramsey Andrew P. Woolnough Marc Franch Gustavo A. Llorente Albert Montori Xabier Buenetxea Asier R. Larrinaga Matthieu Lasceve Alberto Álvarez José María Traverso Aitor Valdeón Ariñe Crespo Virginia Rada Enrique Ayllón Vicente Sancho J. Ignacio Lacomba José Vicente Bataller Miguel Lizana 《Biological invasions》2017,19(9):2739-2750
38.
39.
Guillem Chust J. Icarus Allen Laurent Bopp Corinna Schrum Jason Holt Kostas Tsiaras Marco Zavatarelli Marina Chifflet Heather Cannaby Isabelle Dadou Ute Daewel Sarah L. Wakelin Eric Machu Dhanya Pushpadas Momme Butenschon Yuri Artioli George Petihakis Chris Smith Veronique Garçon Katerina Goubanova Briac Le Vu Bettina A. Fach Baris Salihoglu Emanuela Clementi Xabier Irigoien 《Global Change Biology》2014,20(7):2124-2139
Ocean warming can modify the ecophysiology and distribution of marine organisms, and relationships between species, with nonlinear interactions between ecosystem components potentially resulting in trophic amplification. Trophic amplification (or attenuation) describe the propagation of a hydroclimatic signal up the food web, causing magnification (or depression) of biomass values along one or more trophic pathways. We have employed 3‐D coupled physical‐biogeochemical models to explore ecosystem responses to climate change with a focus on trophic amplification. The response of phytoplankton and zooplankton to global climate‐change projections, carried out with the IPSL Earth System Model by the end of the century, is analysed at global and regional basis, including European seas (NE Atlantic, Barents Sea, Baltic Sea, Black Sea, Bay of Biscay, Adriatic Sea, Aegean Sea) and the Eastern Boundary Upwelling System (Benguela). Results indicate that globally and in Atlantic Margin and North Sea, increased ocean stratification causes primary production and zooplankton biomass to decrease in response to a warming climate, whilst in the Barents, Baltic and Black Seas, primary production and zooplankton biomass increase. Projected warming characterized by an increase in sea surface temperature of 2.29 ± 0.05 °C leads to a reduction in zooplankton and phytoplankton biomasses of 11% and 6%, respectively. This suggests negative amplification of climate driven modifications of trophic level biomass through bottom‐up control, leading to a reduced capacity of oceans to regulate climate through the biological carbon pump. Simulations suggest negative amplification is the dominant response across 47% of the ocean surface and prevails in the tropical oceans; whilst positive trophic amplification prevails in the Arctic and Antarctic oceans. Trophic attenuation is projected in temperate seas. Uncertainties in ocean plankton projections, associated to the use of single global and regional models, imply the need for caution when extending these considerations into higher trophic levels. 相似文献
40.
Lissete Sánchez-Magraner Itziar M. D. Posada Nagore Andraka F. Xabier Contreras Ana R. Viguera Diego M. A. Guérin José L. R. Arrondo Hugo L. Monaco Félix M. Goñi 《The Journal of membrane biology》2014,247(2):155-165
Human phospholipid scramblase 1 (SCR) is a 318 amino acid protein that was originally described as catalyzing phospholipid transbilayer (flip-flop) motion in plasma membranes in a Ca2+-dependent, ATP-independent way. Further studies have suggested an intranuclear role for this protein in addition. A putative transmembrane domain located at the C terminus (aa 291–309) has been related to the flip-flop catalysis. In order to clarify the role of the C-terminal region of SCR, a mutant was produced (SCRΔ) in which the last 28 amino acid residues were lacking, including the α-helix. SCRΔ had lost the scramblase activity and its affinity for Ca2+ was decreased by one order of magnitude. Fluorescence and IR spectroscopic studies revealed that the C-terminal region of SCR was essential for the proper folding of the protein. Moreover, it was found that Ca2+ exerted an overall destabilizing effect on SCR, which might facilitate its binding to membranes. 相似文献