Expression of p21ras-related protein in the plasma and tissue of patients with adenomas and carcinomas of the colon

Over-expression of p21 ras-related protein was determined in the plasma by immunoblotting and in the tissue by immuno-histochemistry in a cohort of patients undergoing colonoscopy. In the plasma samples, p21 ras over-expression was detected in: 9% (4/47) of normal controls; 21% (13/61) of individuals with normal colonoscopies but with a prior history of colonic neoplasia; 12% (4/33) of small adenoma patients, 29% (6/21) of large adenoma patients; 63% (5/8) of carcinoma-in-adenoma patients; 50% (2/4) of Dukes' A carcinoma patients; and 20% (2/10) of Dukes' B-D carcinoma patients. In the tissue samples, p21 ras over-expression was detected in: 25% (2/8) of small adenoma patients; 44% (4/9) of large adenoma patients; 100% (4/4) of carcinoma-in-adenoma patients; and 33% (1/3) of Dukes' B-C carcinoma patients. For matched plasma-tissue pairs, there was a statistically significant correlation for p21 ras over-expression (R = 0.47, p = 0.02).     

癫痫对大鼠学习记忆的影响及与生长抑素的相关性研究

对听源性癫痫大鼠进行迷宫训练,观察癫痫发作对大鼠光分辨学习作业的成绩以及用放射免疫分析法测定的大鼠部分脑区中生长抑素含量的影响。结果表明：（１）癫痫发作对大鼠记忆阶段有明显的抑制作用（Ｐ＜０．０１）;（２）癫痫发作后,在大鼠信息提取明显障碍的同时,其颞叶皮层、下丘脑、垂体及海马中生长抑素的含量明显增加（Ｐ＜０．０５－０．０１）。结果提示：癫痫发作影响大鼠学习记忆过程,在该过程中生长抑素含量发生改变。     

北方稻田生态系统水量平衡及水分效率研究

１９９３～１９９５年研究了５种不同模式水稻田生态系统水量平衡及水分效率结果表明,不同水稻田模式其总耗水量之间有明显差异,其中节水模式和节水节肥模式较常规模式节省灌溉水达１５～２３％,水分生产效率增加３０％以上．各模式蒸发蒸腾耗水量在同一生长季内基本相同．田间结构及调控管理对其无明显影响实测水稻生育期田间蒸发蒸腾量与计算的可能蒸发蒸腾量相差不过５％。     

特种油料植物的种质资源研究

本文对特种油料植物的种质资源研究进行综述,这些植物包括含长链脂肪酸的Crambe,Limnanthes,Lunaria,短链脂肪酸的Cuphea,Lauraceae,羟基脂肪酸的Lesquerella,环氧脂肪酸的Vernonia,Stokesia,以及液体蜡酯的simmondsia等科、属的植物。     

低能量He—Ne激光血管内照射治疗脑血栓形成35例临床观察

本文报道了用低能量Ｈｅ—Ｎｅ激光血管内照射治疗脑血栓形成３５例,与常规药物治疗组３０例对照,结果表明治疗组明显优于对照组（Ｐ＜０．０５）,二者有显著性差异．     

重组人白细胞介素－2在离子色谱分离时的复性

采用ＨＰＩＥＣ应用ＣＭ交换柱分离纯化Ｅ．ｃｏｌｉ工程菌表达的ｒＩＬ－２,与空气氧化复性相比较,又有１倍以上的变性ｒＩＬ－２在色谱过程中得到复性,且溶液ｐＨ影响ｒＩＬ－２的复性和纯化效果：ｐＨ７．０时ｒＩＬ－２复性率高于ｐＨ８．０时,但纯度明显低于ｐＨ８．０时分离的ｒＩＬ－２．     

Rapid recovery of photosynthetic cell suspension cultures following heterotrophic bleaching

Summary Seven suspension-cultured lines of five different species (Amaranthus powellii Datura innoxia, Glycine max, Gossypium hirsutum, andNicotiana tabacum × Nicotiana glutinosa fusion hybrid), which had been grown under photomixotrophic conditions, were placed under heterotrophic conditions (darkness and media with 3% sucrose or starch) where the chlorophyll levels declined to near zero. After three transfers over a 70-d period, the cells were placed back into photomixotrophic or photoautotrophic conditions where regreening occurred rapidly and continued growth was observed. This rapid adaptation to photosynthetic conditions contrasts with the original initiation process for these cultures, which required many months and an apparent selection since many of the original cells died. Thus, these seven photosynthetic cell suspension cultures appear to be different from the original cultures due possible to genetic or adaptive changes.     

人前列腺生长因子的分离纯化及生物活性鉴定

从人良性增生前列腺组织中经硫酸铵沉淀和肝素－琼脂糖凝胶层析纯化出人前列腺生长因子（ｈＰＧＦ）,纯化倍数约１０００倍,ＳＤＳ－ＰＡＧＥ和等电聚焦电泳示分子量约为１７ｋＤ、等电点同标准ｂＦＧＦ,利用分离培养的人前列腺间质成纤维细胞进行活性鉴定,发现以１．３～１．７ｍｏｌ／ＬＮａＣｌ洗脱部分为ｈＰＧＦ,活性最高,对间质成纤维细胞有显著刺激增殖作用。     

广州抽水蓄能电站蓄水库蓄水初期浮游生物调查

对我国首座核电站配套工程的抽水蓄能电站水库蓄水初期的浮游生物进行了调查。蓄水水库由上下两水库组成。两水库的浮游生物种类组成显著不同。上水库浮游植物共记录到３０种（属）,浮游动物１２种（属）;下水库浮游植物１９种（属）;浮游动物２５种（属）。上下两水库浮游生物数量均较贫乏,表明浮游生物群落尚处于初期发育阶段,但在局部浅水处已出现小规模的蓝藻水花,认为是水库蓄水初期必然的结果     

Production and cytogenetics of intergeneric hybrids between Brassica napus and Orychophragmus violaceus

The intergeneric hybrid between Brassica napus and Orychophragmus violaceus was obtained by means of embryo culture technique with the latter as the pollen parent. The hybrid was morphologically intermediate between its parents, but could produce a lot of seeds when selfed. Somatic separation of the genomes from the two parental species was observed during the mitotic divisions of some of the hybrid cells. Thus, the hybrid became the mixoploid in nature, consisting of haploid and diploid cells of B. napus, and a nuclear — cytoplasmic hybrid, with the cytoplasm of B. napus and the nuclei of O. violaceus, and the hybrid cells. Pollen mother cells with 19, 12 and 6 bivalents, respectively, were produced by the hybrid. From the selfed progeny of the hybrid, mainly two kinds of plants, B. napus and the hybrid, were found. The hybrid plants of the selfed progeny again produced two kinds of plants, B. napus and the hybrid.