三种益生菌混合培养及共生机理研究

确定酪酸菌、肠膜芽孢杆菌与粪肠球菌三种菌混合工艺.研究了三种菌混合培养的生长曲线,混合批式传49代、混合连续培养考察三种菌的共存稳定性.三种菌无细胞上清液彼此之间均有促进生长作用,混合培养液的菌数较单独培养增加,在稀释率0.042/h,填充床连续培养11d,三种菌可稳定共存,但批式传49代过程中,肠膜芽孢杆菌有消失现象,而酪酸菌、粪肠球菌均较传代前增加了100倍.平板打孔生长圈法、点种法实验分别表明酪酸菌、肠膜芽孢杆菌对粪肠球菌有显著的促进作用,连续培养较批式传代可更好的研究菌际关系.并得到简单易行的复方益生菌剂组方方法.     

生态经济区划研究——以西北6省为例

汇集西北6省区近300个气象站点的多年气象数据,400多个县市的土地利用和社会经济统计数据,提取降水、气温、湿度、土地利用类型、人口、GDP、矿产资源、产业结构(三次产业)等要素,设计了水热状况、人类活动强度、工业化程度、城镇化程度等指标,借助GIS工具,通过要素指标的空间分异特征研究,构筑指标体系,进行了以县域尺度为最小空间单元的生态经济区划研究。根据西北地区县域尺度上的生态环境状况和人类活动状况,将西北划分为3级27个生态经济区。     

A novel piezoelectric quartz micro-array immunosensor based on self-assembled monolayer for determination of human chorionic gonadotropin

A novel multi-channel 2 x 5 model of piezoelectric quartz micro-array immunosensor has been developed for quantitative detection of human chorionic gonadotropin (hCG) in serum or urine samples. Every crystal unit of the fabricated piezoelectric hCG micro-array immunosensor can oscillate independently without interfering each other. A 2 x 5 model of micro-array immunosensor as compared with a one-channel immunosensor can provide eight times higher detection speeds for hCG assay. The anti-hCG antibody is deposited on the gold electrode's surface of 10 MHz quartz AT-cut crystal by self-assembled technique using sulfosuccinimidyl 6-[3'-(2-pyridyldithio) propionamido] hexanoate (Sulfo-LC-SPDP), and serves as an antibody recognizing layer. The highly ordered self-assembled monolayers (SAM) ensure well-controlled surface structure and offer many advantages to the performance of the sensor. Compared with conventional antibody immobilization methods, the amount and the reaction activity of antibody monolayer coated by the SAM binding are bigger than those by the SPA method, and less non-specific binding caused by other analytes in sample is found. Under the optimized experimental conditions, the results showed that micro-array immunosensor quantitatively detected serum or urine hCG in the range of 2.5-500 mIU/ml with high precision (CV<5%); other hormones in human serum and urine did not interfere with the determination markedly. Serum and urine samples of 60 patients were detected by the micro-array immunosensor, and the results agreed well with those given by the commercial radioimmunoassay test kit, with correlation coefficient of 0.92. After regeneration with urea solution the coated immunosensor can be reused five times without appreciable loss of activity.     

糙皮侧耳原基期差异表达基因分析

为解析糙皮侧耳原基期与菌丝期差异表达的基因,本研究以原基期cDNA为检测子（tester）、双核菌丝期cDNA为驱赶子（driver）,采用抑制性消减杂交法（suppression subtractive hybridization,SSH）构建了糙皮侧耳SSH cDNA文库。菌液PCR验证SSH cDNA文库插入cDNA片段后,挑取了2 055个差异转化子,差异转化子经3次反向Northern杂交筛选,得423个信号差异显著的克隆;阳性克隆测序后,经NCBI数据库Blastn和Blastx比对,共得206条差异表达序列（expressed sequence tag,EST）,重复序列去除后,有46个基因参与了细胞急救和防御、能量代谢、转录和蛋白调控、膜蛋白和信号转导,18个基因编码未知功能的推定蛋白,5个无任何同源性的新基因。挑取10个差异表达基因进行半定量RT-PCR,发现这些序列在原基期的表达水平显著高于菌丝期。结果表明,本研究成功构建了糙皮侧耳原基期与菌丝期SSH cDNA文库,为进一步分离糙皮侧耳生长发育相关基因并研究糙皮侧耳的发育机制奠定了基础。     

鲨鱼软骨血管生成抑制因子的纯化和功能

以中国东海鲸鲨软骨为原料,通过盐抽提、丙酮分级沉淀、离子交换层折、分子筛层析、高效液相色谱等步骤,获得鲨鱼软骨血管生成抑制因子－Ｉ（ｓｈａｒｋ ｃａｒｔｉｌｇａｅ ａｎｇｉｏｇｅｎｅｓｉｓ ｉｎｈｉｂｉｔｏｒｙ ｆａｃｔｏｒ－Ｉ,ＳＣＡＩＦ－Ｉ）,对其分子量、抑制血管生成及抑制肿瘤生长活性进行了研究。结果显示ＳＣＡＩＦ－Ｉ分子量１８ｋＤ,在细胞和整体水平上显著抑制新血管生成,显著抑制小鼠肿瘤的生长     

Quality of Life Is Related to Social Support in Elderly Osteoporosis Patients in a Chinese Population

Objective

To explore the association between quality of life and social support in elderly osteoporosis patients in a Chinese population.

Methods

A total of 214 elderly patients who underwent bone mineral density screening were divided into two groups: elderly patients with primary osteoporosis (case group, n = 112) and normal elderly patients (control group, n = 102). Quality of life and social support were compared between the two groups.

Results

Quality of life and social support were significantly different between the case and control groups. The physical function, role-physical, bodily pain, general health, vitality, social-functioning, role-emotional and mental health scores in case group were significantly lower than those in the control group (P < 0.01). The objective support, subjective support, utilization of support, and total scores in case group were significantly lower than those in the control group (P < 0.01). Quality of life and social support were positively correlated in the case group (r = 0.672, P < 0.01).

Conclusion

Quality of life and social support in elderly patients with osteoporosis in China were poorer than in elderly patients without osteoporosis and were positively correlated. Our findings indicate that increased efforts to improve the social support and quality of life in elderly osteoporosis patients are urgently needed in China. Further longitudinal studies should be conducted to provide more clinical evidence to determine causative factors for the observed association between risk factors and outcomes.     

青海湖四种繁殖水鸟活动区域的研究

2006年4-9月,采用彩色标记、无线电遥测和卫星跟踪等方法,对青海湖四种繁殖水鸟斑头雁（Anser indicus）、棕头鸥（Larus brunnicephalus）、渔鸥（L．ichthyaetus）和鸬鹚（Phalacrocorax carbo）的活动区域进行了研究。采用“绳套法”捕捉了45只斑头雁,其中6只于4月安装了无线电发射器,6只于7月安装了卫星发射器;采用“拉网法”捕捉了104只棕头鸥,其中6只于4月安装了无线电发射器;采用“绳套法”捕捉了51只渔鸥,其中2只于4月安装了无线电发射器;采用“扣网法”捕捉了75只鸬鹚,其中6只于5月和6月安装了无线电发射器,4只于8月安装了卫星发射器。通过研究,获得了上述四种繁殖水鸟在青海湖的活动区域,即：斑头雁有3个主要的活动区域,棕头鸥有1个,渔鸥有4个,鸬鹚有2个。其中从鸬鹚岛、蛋岛、布哈河口、铁卜恰河口至泉湾区域是上述四种繁殖水鸟共有的活动区域,该区域也是春秋迁徙季节众多水鸟的重要取食地和停歇地。     

高致病性2型猪链球菌plcR基因敲除株的构建及其相关生物学特性的研究

【目的】构建高致病性2型猪链球菌05ZYH33菌株plcR基因敲除株,通过比较突变株与野生株生物学特性的差异,研究plcR基因在2型猪链球菌致病过程中的作用。【方法】利用同源重组技术敲除plcR基因,多重交叉PCR及RT-PCR鉴定并测序验证。比较野生株与突变株基本生物学特性的差异,小鼠攻毒实验分析plcR基因缺失对细菌毒力的影响。【结果】经RT-PCR证实05SSU0241与05SSU0242共转录,通过多重交叉PCR及RT-PCR证实成功构建plcR基因缺失突变株,基本生物学特性显示突变株的生长速率、菌落形态、溶血活性均无显著改变,小鼠致病性试验结果显示,野生株攻毒的小鼠死亡率为70%,突变株攻毒的小鼠死亡率为40%,毒力较野生株显著降低。【结论】plcR基因作为2型猪链球菌有毒株基因组中特有的外源基因,在细菌致病过程中具有重要作用。     

基于形态性状的淫羊藿属（小檗科）的分支分析

小檗科（Berberidaceae）淫羊藿属（Epimedium Linn.）植物全世界约有55种,中国分布有44种,其余种类则分布于东亚、克什米尔、北非和高加索等地。该属植物具有复杂的形态学变异（例如：单叶、一回三出复叶、二回三出复叶,花茎具两叶、三叶或一叶,花瓣无距、有距、距多样式等）,导致仅依靠直观判断不能对该属系统进行划分。李超等[1]采用数量分类法对该属55种植物的分类结果多数与Stearn[2]25-26和王悦云等[3]的研究结果一致,但部分种类的分类仍存异议,例如：Sect.