L-亮氨酸发酵培养基优化试验

应用Plackett-Burman设计试验从L-亮氨酸基础发酵培养基的10种成分中筛选出5种重要成分,然应用响应面分析试验确定5种重要成分的最适用量。培养基优化后摇瓶分批发酵72h产L-亮氨酸18.05g/L     

山西太岳山南部针阔混交林群落特征及空间分布格局

为探究针阔混交林群落的空间分布特性、种间关系及演替规律,该研究通过点格局分析方法中的Ripley’L函数对山西太岳山南部1 hm~2针阔混交林中主要乔木种群的空间分布格局及空间关联性进行了研究。结果表明:样地内共调查木本植物41科71属76种,以蔷薇科、毛茛科、唇形科、豆科、菊科、百合科为主,科的分布区类型主要以世界广布为主,属的分区类型以北温带为主;辽东栎(Quercus wutaishanica)、槲树(Quercus dentata)、油松(Pinus tabuliformis)和山杨(Populus davidiana)是该群落的主要树种,样地内的林木总径级结构与主要乔木种群的径级结构相似,呈近似正态分布。辽东栎和槲树的空间分布相似,随尺度增大种群的聚集性减弱并逐渐表现出随机分布的格局特征;油松和山杨在一定尺度范围内呈现聚集分布;主要树种均在一定尺度上表现为两两正相关,且槲树与油松、山杨在整个尺度内都表现为显著正相关。山西太岳山南部针阔混交林中各个乔木种群处于相对稳定的状态。     

刺参养殖池塘水体中浮游病毒的丰度

运用荧光显微技术,于2007年3～11月,对大连市附近4个地区的刺参养殖池塘及相应的海域进行了浮游病毒丰度的监测和分析,对刺参养殖池塘生态系统的浮游病毒丰度在时间、空间分布上的变化进行了探讨。荧光显微观察结果显示,刺参养殖池塘浮游病毒在时间和空间分布上均存在极显著差异（P〈0.01）,8月中旬平均丰度达到峰值,为2.54×10^10个/L,7月下旬浮游病毒的平均丰度最低,为1.43×10^9个/L。复州湾海域的浮游病毒丰度显著高于其他地区,该地区浮游病毒丰度平均达到1.17×10^10个/L,夏家河子地区浮游病毒丰度最低,平均为3.89×10^9个/L。同一刺参养殖池塘中部区域的浮游病毒丰度高于进水和排水口。刺参养殖池塘水体中浮游病毒丰度与养殖池塘所处的海区位置、养殖池塘的密度密切相关。     

Three-Dimensional Trabecular Alignment Model

The Shear-slip Mesh Update Method (SSMUM) is being used in flow simulations involving large but regular displacements of one or more boundaries of the computational domain. We follow up the earlier discussion of the method with notes on practical implementation aspects. In order to establish a benchmark problem for this class of flow problems, we define and report results from a two-dimensional viscous flow around a rotating stirrer in a square chamber. The application potential of the method is demonstrated in the context of biomedical design problem, as we perform an analysis of blood flow in a centrifugal left ventricular assist device, or blood pump, which involves a rotating impeller in a non-axisymmetric housing.     

侵染花生的辣椒褪绿病毒S RNA全序列分析

番茄斑萎病毒属(Tospovirus)是布尼亚病毒科(Bunyaviridae)中植物病毒组成的一个属, 病毒粒子为球状,直径80～110nm,粒体外层由一层脂质包裹.基因组属于负单链RNA,由三个片段组成,分别被称为L RNA、M RNA、和S RNA.L RNA为负链、含单个开放阅读框架(ORF),M RNA和S RNA均为双义RNA, 有2个ORF,反向相连.     

单纯疱疹病毒Ⅰ型糖蛋白D在酵母中的表达

从提取的HSV-1基因组中扩增得到编码gD蛋白胞外区1～314aa的基因gDt,将其插入毕赤酵母表达质粒pPIC9K的醇氧化酶(AOX1)启动子下游,构建携带gDt的重组载体,经电转化GS115菌株和G418筛选,得到了高效分泌表达gD蛋白的毕赤酵母菌株,表达量达到250mg/L,该目的蛋白可被gD单抗(1-I-9)特异性识别.表达产物经离子交换、金属螯合、分子筛柱层析纯化后得到纯度较高的重组蛋白.重组gD蛋白免疫BALB/c小鼠可诱生一定水平的特异性抗体,表明该蛋白具有较好的免疫原性,能够诱导小鼠产生体液免疫应答.     

Construction of a trivalent candidate Shigella vaccine strain with host-vector balanced-lethal system

A trivalent liveShigella vaccine candidate FSD01 against S.flexneri 2a, S.sonnei and S.dysenteriae I was constructed. This candidate strain was based on the S.flexneri 2a vaccine T32. By homologous recombination exchange, the chromosomalasd gene of T32 was site-specifically inactivated, resulting in the strain unable to grow normally in LB broth, while anotherasd gene of S.mutans was employed to construct an Asd⁺ complementary vector. This combination ofasd ^- host/Asd⁺ vector formed a balanced-lethal expression system in T32 strain. By use of this system, two important protective antigen genes coding for S.sonnei Form I antigen and Shiga toxin B subunit were cloned and expressed in T32, which led to the construction of trivalent candidate vaccine FSD01. Experimental results showed that this strain was genetically stable, but its recombinant plasmid was non-resistant. Moreover, it was able to effectively express trivalent antigens in one host and induce protective responses in mice against the challenges of the above threeShigella strains.     

番茄‘Ls-89’和‘坂砧2号’幼苗活性氧代谢对南方根结线虫侵染的反应

为了解番茄(Lycopersicon esculentum)砧木幼苗活性氧代谢与抗南方根结线虫(Meloidogyne incognita)的关系,以高感品种‘Ls-89’与高抗品种‘坂砧2号’为材料,采用盆栽人工接种法,研究了南方根结线虫侵染对番茄砧木幼苗活性氧代谢的影响。结果表明,未接种南方根结线虫的番茄砧木幼苗,其根系与叶片活性氧水平及相关酶活性在品种间没有显著差异。接种南方根结线虫后,两品种幼苗根系与叶片的O_2·~–生成速率和H_2O_2含量均升高,且‘坂砧2号’显著高于‘Ls-89’,但‘Ls-89’的MDA含量则显著高于‘坂砧2号’。两品种幼苗根系与叶片的SOD活性均在侵染早期降低,以‘坂砧2号’降幅较大;但POD、CAT活性在侵染早期变化不大,至侵染中后期则显著升高。因此,番茄抗性品种砧木幼苗的膜脂抗氧化能力较强,活性氧水平较高,且SOD活性对南方根结线虫侵染敏感。