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81.
三种鱼类生长激素cDNA基因的结构比较研究 总被引:3,自引:0,他引:3
从厦门海区选取3种生长速度不同的鱼类,真鲈、高体Shi、褐菖You,由它们的脑下垂体中分别提取出总RNA,用逆转录PCR方法(RT-PCR)扩增出生长激素cDNA,克隆到pBluescript载体上的EcoRI位点,并分别测定了这3种鱼的成熟生长激素cRNA序列。将由这3种序列推导出的氨基酸序列同已知的8种不同科鱼类的生长激素氨基酸序列进行比较,分析它们序列的同源性,结果表明它们间的同源性与这些鱼 相似文献
82.
乙肝病毒PreSl片段与乙肝表面抗原羧端的融合表达 总被引:4,自引:0,他引:4
我们利用聚合酶链反应法(PCR)得到了编码乙肝病毒肝细胞受体结合位点PreSl(21 ̄47)的基因片段,并将它分别融合到S基因中相应于第175,188和223位氨基酸残基处。所得到的融合基因插入痘苗病毒表达载体pGJP-5后,在哺乳动物细胞CV-1中进行了暂时表达,对融合蛋白的表达、分泌和抗原性的研究表明,3种融合基因均能表达具有S和PreSl双重抗原性的融合蛋白,但融合位点对表达水平和分泌性质有 相似文献
83.
中国大痣小蜂属食植群记述(膜翅目:长尾小蜂科) 总被引:7,自引:1,他引:6
本文报导中国大痣小蜂属Megastigmus食植群Phytophagous group 12种,其中包括5新种:黄杉大痣小蜂Megastigmus pseudotsugaphilus sp.nov.(寄生华东黄杉Pseudotsugagassenii),拟海棠大痣小蜂Megastigmus pseudomali sp.n.(寄主不明),基室大痣小蜂Megastigmus cellus sp.nov 相似文献
84.
XUZIQIN JINGFENJIA 《Cell research》1995,5(2):187-195
An efficient protocol for plant regeneration from protoplasts of hydroxyproline(HYP)resistant cell line of Onobrychis viciaefolia was established.In SH medium supplemented with 1mg/L2,4-dichlorophenoxy-acetic acid(2,4-D),0.5mg/L kinetin(KT)and 0.2mg/L naphthalene acetic acid(NAA),the division frequency of protoplastderived cells reached up to over 60%,and microcalli were obtained in 5-6wk.Upon transferring them on agar solidified MS medium plus 2mg/L indole-3-acetic acid (IAA),shoots were induced.After cultivating them on MS medium with or without IAA,roots were regenerated.Chromosome number of all protoplast-regenerated plants examined were normal(2n=28).The protoplast-derived calli and plants grew vigorously on the medium containing 10 mmol/L HYP. 相似文献
85.
This paper describes an approach to seek for mouse c-Myc/Myn proteins-bound specific sequences among genomic DNA.cDNA fragment of myn gene was obtained through RT-PCR technique from RNA of NIH3T3 cells.DNA fragments encoding BR/HLH/LZ structure of Myc and Myn proteins were cloned in frame into pGEX-2T vector respectively.Fusion GST-Myc and GST-Myn synthesized in E.coli hosts showed affinity to CACGTG E-box DNA and subsequently interacted with genomic fragments prepared through whole-genome-PCR.A PCR-assisted procedure which combines protein-DNA interaction and affinity chromatography was designed to enrich Myc/Myn bound DNA.At least two genomic DNA fragments obtained exhibit specifical binding capacity to Myc/Myn complex but not to GST alone.Significance of the work and of the technique itself as well asidentification of the DNAs are discussed. 相似文献
86.
午间强光胁迫下SOD对大豆叶片光合机构的保护作用 总被引:5,自引:0,他引:5
晴天田间大豆叶片Pn与Pr均表现出明显的日变化,Pn日变化曲线里双峰型,中午前后降低。Pr随日照强度的增加而增加,至上午11时左右达最大值,然后缓慢下降。普通空气及低氧空气中的AQY均在中午前后降低。SOD活性也有明显的日变化,最高值出现在下午16时左右。强光下喷施SOD抑制剂DDTC明显降低Pn及低氧空气中的AQY;而在低于叶片光合作用饱和光强下喷施同样浓度DDTC则对Pn及低氧空气中的AQY无明显影响。中午前后SOD活性及Pr的增加对于保护光合机构免受强光的破坏具有重要意义。 相似文献
87.
新疆六个民族指纹白线的调查研究The hand Print of Six Nationalities in Xinjiang 总被引:7,自引:0,他引:7
张致中 张 虎晁招相 朱叔云ZHANG Zhi-Zhong ZHANG Hu CHAO Zhao-Xiang ZHU Shu-Yun张绍良 ZHANG Shao-Liang 张 倩 ZHANG Qian 《遗传》1994,16(1):5-7
本文首次报道了新家6各民族6134人(南3181人、女2953人)的指纹白线出现频率。结果表明,白线频率女高于男,民族间亦有差异,其中维吾尔、哈萨克、鸟孜别克族频率接近。塔吉克族居住高寒高原地区,属白色人种,频率明显低于以上3个民族,锡伯族和汉族生活习惯近似,且高于以上4个民族,作者认为,白线出现频率的高低与种族、性别、年龄和群体生长环境及生活习惯有关。We’ve studied 6134 individuals (male 3181, fimale 2953) of six nationalities in Xinjiang.The result is that the white line of hand print is associated with rae,sex,age,grow environments of groups and life habbits.The ifference of races is:The appearant rates of white line of Weivuerite,Hasakese and Wuzibese are near,Tajike’s rate is lower than xiboman’s and han are higher than others. 相似文献
88.
本文以凝胶等电聚焦电泳方法测定150例原发性肝癌患者、100例良性肝病患者和112例正常人血清α1AT(Pi)表型的分布。统计学检验说明:(1)我们的烽样结果符合Hardy-Weinberg平衡定律;(2)M2等位基因的相对危险度在2.18-21.96,平均为7.502±3.836。因此,M2的出现在肝癌的检测中有重要意义。 相似文献
89.
Resistance to potato leafroll virus (PLRV), potato virus Y (PVYo) and potato virus X (PVX) was studied in symmetric and asymmetric somatic hybrids produced by electrofusion between Solanum brevidens (2n=2×=24) and dihaploid S. tuberosum (2n=2×=24), and also in regenerants (B-hybrids) derived through protoplast culture from a single somatic hybrid (chromosome number 48). All of the somatic hybrids between 5. brevidens and the two dihaploid lines of potato cv. Pito were extremely resistant to PLRV and PVYoand moderately resistant to PVX, irrespective of their chromosome number and ploidy level (tetraploid or hexaploid). Most (56%) of the asymmetric hybrids of irradiated S. brevidens and the dihaploid line of potato cv. Pentland Crown (PDH40) had high titres of PVYosimilar to those of PDH40, whereas the rest of the hybrids had PVYotitres less than a tenth of those in PDH40. Three B-hybrids had a highly reduced chromosome number (27, 30 and 34), but were however as resistant to PLRV, PVYoand PVX as 5. brevidens. Two asymmetric hybrids and one B-hybrid were extremely resistant to PLRV but susceptible to both PVY and PVX. The results suggested that resistance to PLRV in 5. brevidens is controlled by a gene or genes different from those controlling resistance to PVY and PVX, and the gene(s) for resistance to PVY and PVX are linked in S. brevidens. 相似文献
90.
S. Chao C. Baysdorfer O. Heredia-Diaz T. Musket G. Xu E. H. Coe Jr 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,88(6-7):717-721
We report here the results of mapping a set of 92 leaf cDNA clones in maize. The ends of each of these cDNA clones have previously been partially sequenced, and the sequence comparison has revealed the putative function for 28 clones. It is expected that the RFLP map developed using these expressed sequence tags will be of great importance for future maize genome analysis, such as for PCR-based gene mapping or gene function identification.Contribution from the Missouri Agricultural Experiment Station. Journal Series N. 12,019. 相似文献