单鼻孔-蝶窦入路垂体瘤切除术的体会

目的：探讨经单鼻孔-蝶窦入路垂体瘤切除手术的效果。方法：应用单鼻孔-蝶窦入路切除22例垂体瘤患者。结果：22例患者手术均顺利进行并于术后3月均获随访,全切除14例,次全切除5例,部分切除3例。结论：经单鼻孔-蝶窦入路显微切除垂体瘤属于微侵袭及功能保护性手术,符合现代神经外科的发展方向,严格把握适应症及禁忌症,完善手术技巧是提高手术效果的关键。     

响应面法优化灵芪菌质多糖固态发酵条件

采用单因素试验和响应面分析法优化灵芪菌质多糖固态发酵条件.优化后发酵条件为:采用药性固体发酵培养基,初始pH6.5,接种量10.16ml/100g,发酵温度30.65℃,相对湿度59.54％,发酵周期28d.利用此优化条件进行灵芪菌质多糖发酵,灵芪菌质多糖含量可达9.12±0.09mg/g,较优化前8.06±0.07 ...     

CBR2激活与小胶质细胞的活化和损伤的关系

目的:探讨大麻素CBR2受体激动剂AM1241预处理对脂多糖(LPS)和γ-干扰素(IFN-γ)所致炎症反应对小胶质细胞活化和损伤的影响。方法:联用LPS和IFN-γ作为小胶质细胞损伤模型,将细胞分为Control组、AM1241组、LPS/IFN-γ组和AM1241+LPS/IFN-γ组;AM1241组和AM1241+LPS/IFN-γ组经AM1241预处理2h,LPS/IFN-γ组和AM1241+LPS/IFN-γ组用含LPS和IFN-γ的培养基培养24h。采用MTT法检测细胞代谢率,硝酸还原酶法检测细胞培养液中一氧化氮(NO)释放量,酶联免疫吸附剂测定细胞培养基中炎症因子释放量,倒置相差显微镜观察细胞形态。结果:与LPS/IFN-γ组相比,AM1241+LPS/IFN-γ组细胞代谢率明显升高(P<0.05),NO、TNF-α、IL-1β和IL-10释放量明显减少(P<0.05),活化和损伤程度明显减轻。结论:大麻素CBR2受体激动剂AM1241预处理可减轻LPS和IFN-γ对小胶质细胞的活化和损伤。     

阻塞型睡眠呼吸暂停低通气综合征与代谢综合征组分关系探讨

目的:探讨阻塞型睡眠呼吸暂停低通气综合征(OSAHS)与代谢综合征(MS)组分关系。方法:对我院2003年1月至2010年7月39例诊断为OSAHS住院患者进行回顾性调查,30例同期住院病人为对照组,均记录年龄、性别,测量身高、体重、血压,检测空腹血糖、血脂,分析OSAHS患者合并MS组分情况。结果:1.OSAHS组与对照组比较,体重指数(BMI)、血压、血清甘油三酯、总胆固醇、低密度脂蛋白胆固醇、载脂蛋白A1升高,高密度脂蛋白胆固醇降低(P<0.05);2.OSAHS组与对照组相比较,无MS组分比例低于对照组,差异有统计学意义(P<0.05);3.OSAHS组与对照组相比较,OSAHS组合并MS组分,包括BMI≥25Kg/m2,血脂紊乱的比例,均高于对照组,差异有统计学意义(P<0.05)。结论:OSAHS患者易合并代谢综合征组分。     

单鼻孔- 蝶窦入路垂体瘤切除术的体会

目的:探讨经单鼻孔-蝶窦入路垂体瘤切除手术的效果。方法:应用单鼻孔-蝶窦入路切除22例垂体瘤患者。结果:22例患者手术均顺利进行并于术后3月均获随访,全切除14例,次全切除5例,部分切除3例。结论:经单鼻孔-蝶窦入路显微切除垂体瘤属于微侵袭及功能保护性手术,符合现代神经外科的发展方向,严格把握适应症及禁忌症,完善手术技巧是提高手术效果的关键。     

关爱解剖学教师生命，远离肿瘤

解剖学是重要的基础医学学科之一。由于解剖学教师常年接触福尔马林,长期在弥散有甲醛的环境中工作,这类人群恶性肿瘤发病率明显高于正常人群。关爱这一特殊战线上默默奉献的的生命,让他们远离肿瘤是我们需要重视的问题。     

广东地区花绒寄甲替代寄主的研究

将花绒寄甲Dastarcus heloporoides Fairmaire幼虫接于替代寄主黄粉甲Tenebrio molitor、吉丁虫Buprestidaesp和大麦虫Zophobas morio,结果表明,黄粉甲幼虫、蛹和成虫均能繁育出花绒寄甲成虫,平均羽化率能达到20%以上,是较好的替代寄主.用黄粉甲繁育的花绒寄...     

重组人血小板生成素早期干预救治重症急性放射病猴的实验研究

重组人血小板生成素（rhTPO）是一种能促进巨核系祖细胞增殖、分化生成血小板的造血因子,研究表明它能促进射线照射小鼠造血功能恢复,前期工作证明rhTPO早期干预可显著提高致死剂量照射小鼠的活存率.本文以7.0Gy照射恒河猴为重度骨髓型急性放射病（ARS）模型,研究了rhTPO早期干预对重症ARS的治疗作用,并与WR2721和＂500＂的辐射防护作用进行了比较,结果发现rhTPO早期干预可明显促进ARS猴造血功能恢复,改善ARS猴症状,简化对症治疗措施,提高重度骨髓型ARS猴活存率,其对重度骨髓型ARS的防治作用优于现有的辐射防护药WR2721和＂500＂,有望开发成安全有效的新型辐射防治药物.     

Association of adiponectin SNP+45 and SNP+276 with type 2 diabetes in Han Chinese populations: a meta-analysis of 26 case-control studies

Recently, many studies have reported that the SNP+45(T>G) and SNP+276(G>T) polymorphisms in the adiponectin gene are associated with type 2 diabetes (T2DM) in the Chinese Han population. However, the previous studies yielded many conflicting results. Thus, a meta-analysis of the association of the adiponectin gene with T2DM in the Chinese Han population is required. In the current study, we first determined the distribution of the adiponectin SNP+276 polymorphism in T2DM and nondiabetes (NDM) control groups. Our results suggested that the genotype and allele frequencies for SNP+276 did not differ significantly between the T2DM and NDM groups. Then, a meta-analysis of 23 case-control studies of SNP+45, with a total of 4161 T2DM patients and 3709 controls, and 11 case-control studies of SNP+276, with 2533 T2DM patients and 2212 controls, was performed. All subjects were Han Chinese. The fixed-effects model and random-effects model were applied for dichotomous outcomes to combine the results of the included studies. The results revealed a trend towards an increased risk of T2DM for the SNP+45G allele as compared with the SNP+45T allele (OR = 1.34; 95% CI, 1.11–1.62; P<0.01) in the Chinese Han population. However, there was no association between SNP+276 and T2DM (OR = 0.90; 95% CI, 0.73–1.10; P = 0.31). The results of our association study showed there was no association between the adiponectin SNP+276 polymorphism and T2DM in the Yunnan Han population. The meta-analysis results suggested that the SNP+45G allele might be a susceptibility allele for T2DM in the Chinese Han population. However, we did not observe an association between SNP+276 and T2DM.     

多形汉逊酵母研究进展

作为研究甲醇代谢、过氧化物酶体稳态和硝酸盐吸收的模式生物,多形汉逊酵母近年来在基础研究领域日益受到重视。在工程应用领域,利用多形汉逊酵母表达真核外源基因有特殊的优势。譬如容易得到高拷贝,在含油酸的培养条件下能够表达膜蛋白等。已有多种外源蛋白在多形汉逊酵母系统中得到表达。本文综述了多形汉逊酵母的基本生物学性质、基础研究领域概况及其在外源基因表达方面的特点和进展。