红腹锦鸡胃肠道内分泌细胞的免疫组织化学定位

应用ABC免疫组织化学方法,对红腹锦鸡(Chrysolophus pictus)胃肠道5-羟色胺(5-hydroxtryptamine,5-HT)、胃泌素(gastfin,GAS)、生长抑素(somatostatin,ss)3种内分泌细胞的分布密度和形态进行了观察.结果显示,5-HT细胞在空肠和直肠分布密度最高,回肠和盲肠次之,十二指肠较少,腺胃和肌胃最少;GAS细胞在十二指肠和直肠分布密度最高,其次是空肠和盲肠,腺胃部最低,肌胃则呈免疫阴性;SS细胞数量较少,在直肠、盲肠处分布密度相对高,其次是十二指肠和空肠,腺胃部最低,肌胃则呈免疫阴性.3种内分泌细胞的形态多样,有圆形、椭圆形、锥体形、杆状和不规则形,其中以圆形、椭圆形为主.细胞分布于固有膜、黏膜上皮细胞基部、黏膜上皮细胞之间、腺泡上皮细胞基部或腺泡上皮细胞之间.红腹锦鸡胃肠道内分泌细胞的形态与其内、外分泌功能是相适应的.     

基于遗传算法的光合细菌过氧化物酶电泳分析方法的建立

建立光合细菌中过氧化物酶的酸性聚丙烯酰胺凝胶电泳(A-PAGE)分析方法。以电泳图谱的谱带数和灰度为指标, 运用正交试验和遗传算法对该方法的电泳条件进行优化。实验结果表明, 当凝胶中Triton X-100含量为0.31%、菌体破碎时间为19 min、样品中Triton X-100含量为0.79%时, 可获得清晰的沼泽红假单胞菌过氧化物酶图谱; 当凝胶中Triton X-100含量为0.27%、菌体破碎时间为16 min、样品中Triton X-100含量为0.76%时, 可获得清晰的球形红细菌过氧化物酶图谱。本方法为光合细菌中过氧化物酶的分析提供了一种新的、简单、快速的手段。     

哺乳动物印记域DLK1-DI03的研究进展

DLK1-D103印记域定位于人14号染色体、小鼠12号染色体及绵羊18号染色体远端,在真哺乳亚纲动物中印记保守.该印记域包含3个编码蛋白的父系表达基因DIk1、Rt11和Di03以及若干大小不同的母系表达印记非编码RNA,如miRNAs、snoRNAs和大型非编码RNA Gtl2等.人和小鼠该印记域内印记基因剂量的改变将导致严重的表型异常甚至胚胎致死,暗示正常的发育需要域内印记基因的正常表达.文章重点论述了哺乳动物DLK1-DI03印记域的印记调控机制和域内印记基因及其功能的研究进展.     

眼镜蛇毒对大鼠弓状核nNOS表达的影响

目的探讨眼镜蛇毒对大鼠弓状核神经元型一氧化氮合酶(neuronal nitric oxide synthase,nNOS)表达的影响。方法采用免疫组织化学方法分别显示眼镜蛇毒组、生理盐水组和正常对照组弓状核的nNOS表达。结果与生理盐水组和正常对照组相比,眼镜蛇毒组弓状核nNOS阳性神经元数量明显减少,细胞平均灰度值明显升高(P0.05)。结论眼镜蛇毒能够使大鼠弓状核nNOS表达降低。     

模拟增温对川西亚高山西类针叶林土壤酶活性的影响

采用开顶式生长室(open top chamber,OTC)模拟增温,同步监测了亚高山人工针叶林和天然针叶林表层土壤温、湿度的变化,以及模拟增温初期土壤转化酶、脲酶、过氧化氢酶和多酚氧化酶活性的变化.结果表明:在整个生长季节中,OTC使人工林和天然林5 am土壤日平均温度分别增加0.61 oC和0.56 ℃,10 am体积含水量分别下降4.10%和2.55%;模拟增温增加了土壤转化酶、脲酶、过氧化氢酶和多酚氧化酶活性.增温与林型的交互作用对土壤脲酶和过氧化氢酶活性有显著影响,而对转化酶和多酚氧化酶影响不显著.增温对过氧化氢酶活性的影响与季节变化相关.在各处理下,天然林土壤酶活性显著高于人工林.土壤酶活性季节动态与土壤温度有着较大相关性,而与土壤水分季节变化关系不明显.模拟增温易于增加土壤酶活性,但增温效应和林型、酶种类和季节变化有一定关系;亚高山针叶林土壤酶活性主要受控于土壤温度,而与土壤水分关系不大.     

Angiotensin II induces NF-kappa B activation in HUVEC via the p38MAPK pathway

Angiotensin II (Ang II) is the main active peptide of the renin–angiotensin system (RAS), producing a number of inflammatory mediators that lead to endothelial dysfunction and the progression of atherosclerosis. Ang II-induced NF-κB nuclear translocation plays a pivotal role in this response. This study examines the NF-κB activation mechanism elicited by Ang II in human umbilical vein endothelial cells (HUVEC). Electrophoretic mobility shift assays and Western blotting revealed that Ang II, signaling via AT₁, produces a time-dependent increase in NF-κB DNA binding and IκB degradation. These results also demonstrate that Ang II leads to MAPK phosphorylation and p38MAPK pathway-induced NF-κB activation. Furthermore, AT₁ is required for p38MAPK phosphorylation induced by Ang II. This study provides evidence that Ang II elicits NF-κB activation via the p38MAPK pathway in HUVEC.     

The Tissue Distribution and Developmental Changes of ghrelin mRNA Expression in Sheep

Male Kazak sheep and Xinjiang fine wool sheep, six for each different age group (days 2, 30, 60, 90 and 120), were used in the present study to investigate the tissue distribution and developmental changes of ghrelin mRNA expression in abomasum; however, there was no 120-day-old Kazak sheep. After measurement of body weight, the tissues such as hypothalamus, pituitary, heart, liver, rumen, reticulum, omasum, abomasum, duodenum, and longissimus dorsi muscle were sampled. And the total RNA of different tissues was extracted to determine the abundance of ghrelin mRNA by RT-PCR and real-time PCR. The results showed that (1) for both breeds, body weight among different ages was significantly different (P<0.05). And from day 30 to 90, the body weight of Kazak was significantly higher than that of Xinjiang (P<0.01); (2) Ghrelin mRNA existed in all the above tissues and was significantly higher in the abomasum than in other tissues (P<0.05); (3) the temporal patterns of abomasum ghrelin mRNA expression in Kazak and Xinjiang were similar. From day 2 to 60 in Kazak and 2 to 90 in Xinjiang, there was a steady increase in the ghrelin mRNA level. By day 60 in Kazak and day 90 in Xinjiang, the level reached a plateau and remained steady. These results also demonstrated that from birth to day 90, ghrelin mRNA level was significantly higher in Kazak than in Xinjiang (P<0.01).     

三分三的离体培养和植株再生

1植物名称三分三(AnisodusacutangulusC.Y.WuetC.Chen)。2材料类别无菌苗叶片。3培养条件(1)种子无菌萌发培养基:MS+6-BA0.5mg·L-1(单位下同);(2)愈伤组织和胚状体诱导培养基:MS+6-BA1.0+2,4-D0.5;(3)生根培养基:MS+NAA1.0。以上培养基均附加3%蔗糖和0.8%琼脂,pH5.8。培养温度为(25±1)℃,光照时间12h·d-1,光照强度为40μmol·m-2·s-1。4生长与分化情况5意义与进展三分三为茄科山莨菪属(也有列入安妮莎属)多年生草本植物,主要分布于我国云南西北部海拔2750～3000m的山区。根、茎、叶和种子均富含生物碱,味苦、辛,性温,有剧毒,具…     

野生稻高产QTL高效表达的光合生理基础

以携带野生稻高产QTL的晚稻新恢复系远恢611所配部分强优势组合为材料,对其部分光合生理指标进行测定的结果表明,远恢611系列组合杂种优势强,穗大粒多,库容量大,具有超高产潜力;后期上面3片功能叶宽大、直立、叶面积大,与茎秆夹角小,不披垂;比叶重大而稳定,不早衰;剑叶净光合速率高,库很大且源较足是远恢611系列组合高产的主要生理原因;也可能是野生稻高产QTL高效表达的重要生理基础。     

斑贝链霉菌接合转移系统的构建及透明颤菌血红蛋白基因的表达对其次级代谢的影响

以pSET152和pHZ1358为出发质粒,通过供体大肠杆菌ET12567(pUZ8002)和S17-1接合转移斑贝链霉菌(Streptomycesbambergiensis),构建和优化了接合转移体系。采用OOE-PCR技术构建含ermEp*与vhb结构基因融合片段的整合型表达载体pBIB2005,转化ET12567(pUZ8002)后,属间接合转移至斑贝链霉菌。通过PCR、CO结合差光谱验证vhb基因在斑贝链霉菌中整合表达。摇瓶发酵显示VHb蛋白能改善菌体对氧的需求,一定程度上促进细胞生长,提高抗生素产量。