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131.
Xin Jinge Zeng Dong Wang Hesong Sun Ning Zhao Ying Dan Yan Pan Kangcheng Jing Bo Ni Xueqin 《Probiotics and antimicrobial proteins》2020,12(1):184-193
Probiotics and Antimicrobial Proteins - Numerous studies have investigated the beneficial effects of Lactobacillus johnsonii strain BS15 on mice and broilers. This study aimed to understand the... 相似文献
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Sura Ali Benjamin Jenkins Jiujun Cheng Briallen Lobb Xin Wei Suhelen Egan Trevor C. Charles Brendan J. McConkey John Austin Andrew C. Doxey 《Molecular microbiology》2020,114(6):979-990
S-layers are paracrystalline proteinaceous lattices that surround prokaryotic cells, forming a critical interface between the cells and their extracellular environment. Here, we report the discovery of a novel S-layer protein present in the Gram-negative marine organism, Pseudoalteromonas tunicata D2. An uncharacterized protein (EAR28894) was identified as the most abundant protein in planktonic cultures and biofilms. Bioinformatic methods predicted a beta-helical structure for EAR28894 similar to the Caulobacter S-layer protein, RsaA, despite sharing less than 20% sequence identity. Transmission electron microscopy revealed that purified EAR28894 protein assembled into paracrystalline sheets with a unique square lattice symmetry and a unit cell spacing of ~9.1 nm. An S-layer was found surrounding the outer membrane in wild-type cells and completely removed from cells in an EAR28894 deletion mutant. S-layer material also appeared to be “shed” from wild-type cells and was highly abundant in the extracellular matrix where it is associated with outer membrane vesicles and other matrix components. EAR28894 and its homologs form a new family of S-layer proteins that are widely distributed in Gammaproteobacteria including species of Pseudoalteromonas and Vibrio, and found exclusively in marine metagenomes. We propose the name Slr4 for this novel protein family. 相似文献
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Qundan Lv Jie Qiu Jie Liu Zheng Li Wenting Zhang Qin Wang Jie Fang Junjie Pan Zhengdao Chen Wenliang Cheng Michael S. Barker Xuehui Huang Xin Wei Kejun Cheng 《The Plant journal : for cell and molecular biology》2020,103(5):1910-1923
Chimonanthus salicifolius, a member of the Calycanthaceae of magnoliids, is one of the most famous medicinal plants in Eastern China. Here, we report a chromosome‐level genome assembly of C. salicifolius, comprising 820.1 Mb of genomic sequence with a contig N50 of 2.3 Mb and containing 36 651 annotated protein‐coding genes. Phylogenetic analyses revealed that magnoliids were sister to the eudicots. Two rounds of ancient whole‐genome duplication were inferred in the C. salicifolious genome. One is shared by Calycanthaceae after its divergence with Lauraceae, and the other is in the ancestry of Magnoliales and Laurales. Notably, long genes with > 20 kb in length were much more prevalent in the magnoliid genomes compared with other angiosperms, which could be caused by the length expansion of introns inserted by transposon elements. Homologous genes within the flavonoid pathway for C. salicifolius were identified, and correlation of the gene expression and the contents of flavonoid metabolites revealed potential critical genes involved in flavonoids biosynthesis. This study not only provides an additional whole‐genome sequence from the magnoliids, but also opens the door to functional genomic research and molecular breeding of C. salicifolius. 相似文献
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Yaxing Chen Chen Qin Jianhan Huang Xin Tang Chang Liu Keru Huang Jianguo Xu Gang Guo Aiping Tong Liangxue Zhou 《Cell proliferation》2020,53(3)
Central nervous system (CNS) maintains a high level of metabolism, which leads to the generation of large amounts of free radicals, and it is also one of the most vulnerable organs to oxidative stress. Emerging evidences have shown that, as the key homeostatic cells in CNS, astrocytes are deeply involved in multiple aspects of CNS function including oxidative stress regulation. Besides, the redox level in CNS can in turn affect astrocytes in morphology and function. The complex and multiple roles of astrocytes indicate that their correct performance is crucial for the normal functioning of the CNS, and its dysfunction may result in the occurrence and progression of various neurological disorders. To date, the influence of astrocytes in CNS oxidative stress is rarely reviewed. Therefore, in this review we sum up the roles of astrocytes in redox regulation and the corresponding mechanisms under both normal and different pathological conditions. 相似文献
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该文报道了产自中国西藏自治区墨脱县境内的线柱苣苔属(Rhynchotechum Blume)中国分布新记录——小花线柱苣苔(R. parviflorum Blume)。该新记录种常生长在林中溪流附近的崖壁以及次生林下阴湿生境中,主要辨别特征为叶基本对生,花萼裂片被绢毛,花梗被黄褐色绒毛,花冠筒较小,子房具短柔毛,果无毛至微柔毛。印度学者于2020年记载为印度新分布,而原始文献中记录的凭证标本采集点位于中国西藏自治区墨脱县境内,故对原文记述的产地信息提出质疑。同时,在前人的研究中部分馆藏的线柱苣苔属植物标本被认定为该新记录种,在此一并提出该新记录种在中国的分布地理信息和详细描述。 相似文献