水稻株形对纹枯病抗性的影响

利用240份源于珍汕97B/明恢63的重组自交系水稻(Oryza sativa L.)群体,连续2年调查纹枯病病级与水稻生育期、株高和叶片长宽等18个株形性状的关系.对株形性状与纹枯病病级进行了偏相关分析.实验结果,只有植株松紧度与病级表型偏相关两年中都达到了显著或极显著水平,倒2叶基角、穗层整齐度等8个性状与病级之间的偏相关只有一年达显著或极显著水平.结合构建的分子标记遗传连锁图谱,对各性状进行QTL定位.在抗纹枯病QTL相近区间仅检测到控制分蘖角、植株松紧度和倒2叶基角的QTLS,未发现其余株形性状QTLs与抗纹枯病QTLs分布在同一染色体上.结果表明,水稻对纹枯病的抗性主要是由本身抗性基因控制,株形对纹枯病抗性表达的影响主要是间接影响,即通过改变田间小气候而影响发病程度.抗纹枯病育种在累加主效抗纹枯病QTLs的同时,也要注重选择不利于纹枯病发展的株形性状.     

植物源调节剂对水稻的影响

植物源调节剂是利用艾蒿为主要原料,首次用提取的混合物研制成的一种植物生长调节剂,使用80mg/L的植物源调节剂混合物水溶液,分别在水稻苗期、拔节期、孕穗期叶面喷洒,清水对照。结果表明:植物源调节剂使水稻增产11.38%,千粒重增加11.45%。株干重增加了17.1%。在灌浆期连续测定表明:相对于CK,穗粒重增加10.9%,叶绿素含量提高14.41%。品质分析结果为:可溶性糖含量提高15.57%、游离氨基酸含量提高18.52%、淀粉含量提高1.59%,可溶性蛋白质降低22.17%、粗纤维降低1.95%、粗脂肪降低1.22%。该植物源调节剂对水稻的生长和产量都有显著的促进效应。     

广东阳春现代樟树树轮宽度变化及其对气候因子的响应

树木年轮方法定年准确、连续性强、分辨率高和易于获取复本等 ,成为全球气候变化研究的重要手段之一。在热带、亚热带地区 ,受树木生理特性的影响 ,树轮研究工作开展较少。通过广东阳春过渡热带现代樟树树木年轮宽度的变化 ,发现秋季降水是影响研究区樟树生长的重要因子。树轮记录的准 4～ 4 .92 a周期的树轮指数变化可能与厄尔尼诺的周期有关 ,即厄尔尼诺引起气候变化对树木生长产生影响。尽管樟树生长快 ,树轮不清晰 ,交叉定年困难 ,但由于研究区存在明显的旱季 ,樟树树轮仍然能够在一定程度上反映气候条件的影响。     

RGD肽与尿激酶B链融合蛋白原核重组表达质粒的构建、表达和功能的初步分析

将化学合成的RGD肽(Arg-Gly-Asp)编码寡核苷酸与尿激酶B链cDNA相连成为融合基因后,克隆至原核表达质粒pBV220中,在P-RP-L启动子的作用下,经42℃热诱导,在大肠杆菌DH5α中获得了融合基因的表达,其表达量占菌体总蛋白的9.2%,表达产物以无活性的包含体形式存在。经变复性处理得到纯化的融合基因的表达产物,经Western blotting分析表明产物具有与天然尿激酶相似的抗原性,体外活性分析显示其具有一定的纤溶和抗血小板聚集的活性。     

Cell Growth and Flavonoids Production in Suspension Culture of Saussurea medusa

Cell suspension cultures were established from Saussurea medusa Maxim. callus cultures. The effects of different rotation speeds of the gyratory shaker, different inoeulum sizes and different pH values of the medium on cell growth and flavonoid formation were studied. The result showed that the optimum rotation speed, inoeulum size and initial pH value of the medium were 90–120 r/min,50– 80 g FW/L and 5.5–6.0 respectively for cell growth and flavonoids formation in the suspension cultures. Sucrose was better than glucose and fructose for the suspension cultures. The optimum concentration of sucrose for cell growth and flavonoid production was 40 g/L, and the concentration of flavonoids could be as high as 1 423.25 mg/L. High performance liquid chromatographic analysis of cell suspension culture extracts showed that the concentrations of jaceosidin and hispidulin in the flavonoids were 22.11% and 0. 15% respectively.     

鼓槌石斛的地理分布与菌根真菌区系组成的相关性

本文研究了兰科石斛属鼓槌石斛的地理分布与菌根真菌区系组成的相关性。采用克隆文库技术对云南省西双版纳和临沧市两个地区的8份野生鼓槌石斛根部样品进行了分析。结果表明,共从两个地区的鼓槌石斛根部获得了14个真菌的可操作分类单元（OTU）,主要隶属于胶膜菌科Tulasnellaceae和小纺锤菌目Atractiellales。分析鼓槌石斛对菌根真菌的专一性和偏好性发现,鼓槌石斛对菌根真菌专一性较低,每个植株都能同时与多种菌根真菌共生;不同地理分布的鼓槌石斛菌根真菌类群存在明显差异,两个地区的鼓槌石斛的菌根真菌中只有1个OTU是相同的,其余均不同。说明菌根真菌可能和鼓槌石斛的生态适应存在一定的相关性。     

Using a non-radioisotopic, quantitative TRAP-based method detecting telomerase activities in human hepatoma cells

INTRODUCTIONTelomerase is a ribonucleoprotein complex that plays a critical role in telomeremaintenance and cellular immortality. Telomerase has been considered as tumordiagnostic marker and potential target for cancer therapyll, 2]. A sensitive, reliableand quantitative assay is of high interest in this field. The development of a very sensitive Telomeric Repeat Amplification Protocol (TRAP) for measuring telomerajseactivity in cell extracts has been proved to be an important tool for…     

细胞松弛素B对多头绒泡菌有丝分裂的影响

将细胞松弛素Ｂ（Ｃｙｔｏｃｈａｌａｓｉｎ Ｂ,ＣＢ）注入同步化的多头绒泡菌原质团,在光镜和电镜下跟踪观察有有丝分裂进程,发现多头绒泡菌进入有丝分裂的时间推迟,与未经ＣＢ处理的样品相比,在Ｓ期注入ＣＢ的样品进入有丝分裂的时间推迟３５ｍｉｎ,在Ｇ２早期注入ＣＢ的样品则推迟２０ｍｉｎ;在Ｇ２中期注入的推迟４５ｍｉｎ;在Ｇ２晚期注入的推迟６０ｍｉｎ,说明抑制肌动蛋白的功能则使有丝分裂受到明显影响。ＣＢ处理     

洛巴口蘑滤液中多糖蛋白复合物的抗肿瘤机制

从洛巴口蘑培养滤液中分离出一种具有抗肿瘤活性的多糖蛋白复合物（PSPC）。PSPC在细胞水平有能力恢复和增强带瘤鼠腹腔浸出细胞的吞噬功能和T细胞的促分裂活性。在分子水平,PSPC能恢复带瘤鼠脾细胞和腹腔浸出细胞内IFN-γ基因的表达水平。另外,PSPC能够下调带瘤鼠脾细胞内“抗细胞因子”TGF－β的表达水平。因此,PSPC的抗肿瘤机制可归咎于寄主介导的免疫调节作用。     

Prevalence and evolution of drug resistance HIV-1 variants in Henan, China

To understand the prevalence and evolution of drug resistant HIV strains in Henan China after the implementation of free antiretroviral therapy for AIDS patients. 45 drug naive AIDS patients, 118 AIDS patients who received three months antiretroviral therapy and 124 AIDS patients who received six months antiretroviral treatment were recruited in the southern part of Henan province. Information on general condition, antiretroviral medicines, adherence and clinical syndromes were collected by face to face interview. Meanwhile, 14ml EDTA anticoagulant blood was drawn. CD4/CD8 T cell count, viral load and genotypic drug resistance were tested. The rates of clinical improvement were 55.1% and 50.8% respectively three months and six months after antiretroviral therapy. The mean CD4 cell count after antiretroviral therapy was significantly higher than in drug naive patients. The prevalence rate of drug resistant HIV strains were 13.9%, 45.4% and 62.7% in drug naive patients, three month treatment patients and six month treatment patients, respectively.The number of resistance mutation codons and the frequency of mutations increased significantly with continued antiretroviral therapy. The mutation sites were primarily at the 103, 106 and 215 codons in the three-month treatment group and they increased to 15 codon mutations in the six-month treatment group. From this result, the evolution of drug resistant strains was inferred to begin with the high level NNRTI resistant strain, and then develop low level resistant strains to NRTIs. The HIV strains with high level resistance to NVP and low level resistance to AZT and DDI were highly prevalent because of the AZT DDI NVP combination therapy. These HIV strains were also cross resistant to DLV, EFV, DDC and D4T. Poor adherence to therapy was believed to be the main reason for the emergence and prevalence of drug resistant HIV strains. The prevalence of drug resistant HIV strains was increased with the continuation of antiretroviral therapy in the southern part of Henan province. Measures, that could promote high level adherence,provide new drugs and change ART regimens in failing patients, should be implemented as soon as possible.