夜蛾科中国一新记录属和二新记录种记述(鳞翅目)

2020年在西藏色季拉山采集过程中发现一中国新记录属长角冬夜蛾属Meganyctycia Hreblay&Ronkay,1998和二中国新记录种:长角冬夜蛾Meganyctycia forcipata Hreblay&Ronkay,1998和褐元夜蛾Avitta rufifrons Moore,[1887].文章详细描...     

The intrinsic contributions of tyrosine, serine, glycine and arginine to the affinity and specificity of antibodies

Synthetic antibody libraries with restricted chemical diversity were used to explore the intrinsic contributions of four amino acids (Tyr, Ser, Gly and Arg) to the affinity and specificity of antigen recognition. There was no correlation between nonspecific binding and the content of Tyr, Ser or Gly in the antigen-binding site, and in fact, the most specific antibodies were those with the highest Tyr content. In contrast, Arg content was clearly correlated with increased nonspecific binding. We combined Tyr, Ser and Gly to generate highly specific synthetic antibodies with affinities in the subnanomolar range, showing that the high abundance of Tyr, Ser and Gly in natural antibody germ line sequences reflects the intrinsic capacity of these residues to work together to mediate antigen recognition. Despite being a major functional contributor to co-evolved protein-protein interfaces, we find that Arg does not contribute generally to the affinity of naïve antigen-binding sites and is detrimental to specificity. Again, this is consistent with studies of natural antibodies, which have shown that nonspecific, self-reactive antibodies are rich in Arg and other positively charged residues. Our findings suggest that the principles governing naïve molecular recognition differ from those governing co-evolved interactions. Analogous studies can be designed to explore the roles of the other amino acids in molecular recognition. Results of such studies should illuminate the basic principles underlying natural protein-protein interactions and should aid the design of synthetic binding proteins with functions beyond the scope of natural proteins.     

抗人尿道高分化鳞癌单克隆抗体的研制

用本室建立的人尿道高分化鳞状上皮癌细胞株(Hus-98)免疫Balb/C小鼠,通过细胞杂交技术及ELISA方法进行筛选,SP免疫组织化学方法进行鉴定,获得10株能稳定分泌、选择性强、效价高的杂交瘤细胞株;同时从乳腺癌组织中提出肿瘤相关抗原行免疫印记实验(Western-blot)。结果6株经4次克隆能稳定分泌抗体Ig,免疫组化显示这些抗体是针对来源于上皮的鳞癌、腺癌细胞膜和细胞浆的,而癌旁正常组织及胎儿组织呈阴性反应;通过western-blot结果显示肿瘤相关抗原分子量为46.414kb。     

体重和温度对褐牙鲆标准代谢的影响

研究表明,褐牙鲆标准代谢率随体重的增加而增加,二者呈幂函数关系;褐牙鲆标准代谢率随温度的升高而增加,二者为指数关系;褐牙鲆标准代谢率与体重和温度的关系用Rs=0.2340W^0.6695e^0.0372T表示。体重和温度对褐牙鲆的标准代谢率没有显著的交互作用。     

Genome-wide identification and abiotic stress responses of <Emphasis Type="Italic">DGK</Emphasis> gene family in maize

Diacylglycerol kinase (DGK) is a kind of phosphokinase that catalyzes the formation of signaling molecule phosphatidic acid. In this study, seven maize (Zea mays) DGK gene family members were identified by an exploration of maize genome via multiple online databases, and designated as ZmDGK1-7, respectively. The proteins encoded by ZmDGKs ranged from 487 to 716 amino acids, and had a molecular weight (MWs) between 54.6 and 80.2 kDa. Phylogenetic analysis revealed that ZmDGKs grouped into three clusters as described for known plant DGK families: Cluster I was composed of three maize DGKs, ZmDGK1, ZmDGK4 and ZmDGK5, cluster II contained ZmDGK6, and the isoforms ZmDGK2, ZmDGK3 and ZmDGK7 fell into cluster III. ZmDGK proteins featured the typical functional domains, while all seven ZmDGKs have a conserved catalytic domain DGKc, only the cluster I ZmDGKs have the DAG/PE binding domain. Most ZmDGK genes showed ubiquitous expression profiles at various developmental stages, while a high relative expression was observed at the tasseling stage. ZmDGK genes exhibited differential expression patterns in response to abiotic stresses including cold, salinity and drought, and all ZmDGK genes were found obviously up-regulated by cold. The distinct roles of ZmDGKs in cold response was also supported by the finding that an accumulation of DGK products–PA under low temperature. This study will help to better understand the roles of DGKs in the development and abiotic stress responses in major crops.     

广州市公园耐湿草本植物及其造景应用

选择广州市具有较长的自然式缓坡驳岸,且植物养护管理良好的6个公园为研究对象,调查其耐湿草本植物种类及其景观应用情况,分析广州公园耐湿草本植物造景的特点与存在的问题。研究表明,在调查区域内共记录有耐湿草本植物77种（含变种、变型和品种）,隶属26科52属,其中一年生草本26种,多年生草本50种,蕨类1种;常用的耐湿草本植物以低矮型和中型草本为主。广州公园的耐湿草本植物应用于水边造景有以下四个特点：①色彩调和,展现水乡风光;②线条变化,增添湿地趣味;③适地适草,体现热带特色;④自然种植,创造观景效果。广州公园建设宜考虑适当增加地带性的多年生耐湿草本植物种类。     

流体剪应力对成骨细胞OPG和ODF表达的影响

通过平板流动腔装置对大鼠成骨细胞施以流体剪应力,研究剪应力作用对成骨细胞中骨保护因子(osteoprotegerin,OPG),破骨细胞分化因子(osteoclast differentiation factor,ODF)基因表达的影响.分别考察了剪应力大小和作用时间,以及单一水平和梯度变化的剪切力加载方式的影响.运用RT-PCR和蛋白质印迹技术检测OPG、ODFmRNA和蛋白质表达的变化,结果显示,剪切力作用下OPG的表达得到促进,ODF的表达受到抑制,mRNA与蛋白质表达的变化一致,这种影响与剪切力的大小和作用时间有关.1.0和1.5N/m2的剪应力作用效果比0.5N/m2明显,梯度变化的作用方式在作用效果上与最后一个梯度水平相当的恒定剪应力单独作用没有显著差异,在加载的24h内剪应力对OPG、ODF表达的影响始终存在.这种影响使得OPG/ODF的平衡向着OPG占优的方向发展,这种变化意味着骨吸收会受到抑制,提示力学刺激可能通过OPG/ODF调控系统对骨代谢平衡进行调控.     

不同EGF和胰岛素水平对体外培养的大熊猫皮肤成纤维细胞的影响

用DME∶Ham sF12(1∶1)培养液,添加3个水平的EGF和2个水平的胰岛素,组合成6种培养体系(CS)分别培养大熊猫皮肤成纤维细胞。通过对细胞生长速度和染色体数目变异率进行测定,测得在添加10μg/ml的胰岛素和40 ng/ml的表皮生长因子的培养体系中,以1.673±0.185×105/ml密度接种细胞,经3.5天,密度达到6.890×105/ml,其生长速度最快;染色体数目为二倍体的百分率为75.77%。综合衡量,CS-5在本研究中更适合大熊猫皮肤成纤维细胞的培养。     

氮、磷、钾肥配施对桂花品种'金玉台阁'开花性状及叶片中叶绿素和营养元素含量的影响

以桂花品种'金玉台阁'(Osmanthus fragrans'Jinyu Taige')盆栽苗为供试材料,采用"3414"肥料效应试验设计方案,研究了不同单株施用量CO(NH2)2(0.0、1.0、2.0和3.0 g)、NH4H2PO4(0.0、1.5、3.0和4.5 g)和KCl(0.0、0.5、1.0、1.5 g)对秋冬生长期(2014年10月至2015年2月)'金玉台阁'开花性状及叶片中叶绿素和营养元素的影响进行了比较和分析,并筛选出适宜的氮、磷、钾肥施用量.结果表明:与CK〔CO(NH2)2、NH4H2PO4和KCl的单株施用量均为0.0 g〕组相比,各氮、磷、钾肥配施处理组'金玉台阁'的单株产花量、花瓣相对含水量、花径以及叶片中叶绿素、全氮、全磷和全钾的含量总体上显著升高.CO(NH2)2单株施用量对'金玉台阁'单株产花量、叶片中叶绿素含量和全氮含量的影响最大,NH4H2PO4单株施用量对这3个指标的影响次之;NH4H2PO4单株施用量对'金玉台阁'叶片中全磷含量的影响最大;KCl单株施用量对'金玉台阁'花瓣相对含水量、花径及叶片中全钾含量的影响最大.在秋冬生长期内叶片中叶绿素含量呈先降低后升高的趋势,均在12月份降至最低值.通过建立'金玉台阁'单株产花量(y)与CO(NH2)2、NH4 H2 PO4和KCl的单株施用量(分别为xN、xP和xK)间的肥料效应模型,得到的三元二次肥料效应模型为y=1.2027+0.7561×xN+0.2632×xP+0.4590×xK-0.2761×xN2-0.1201×xP2-0.5007×xK2+0.1901×xN×xP-0.0960×xN×xK+0.1185×xP×xK.以单株产花量为目标,'金玉台阁'的CO(NH2)2、NH4 H2 PO4和KCl的最佳单株施用量分别为2.58、3.56和0.86 g.