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Production of lutein by the green microalga Chlorella protothecoides grown heterotrophically in a fermentor using glucose as the carbon source and urea as the nitrogen source was investigated.
An unstructured kinetic model was proposed to describe the microalgal culture system including cell growth, lutein formation,
as well as glucose and nitrogen consumption. The inhibition potentials of biomass, product and substrates on growth and lutein
formation were examined and incorporated into the kinetic model. Values of the kinetic model parameters were estimated. The
resulting model predictions were in good agreement with the experimental results. The model can be helpful in scale-up, optimization
and control of the C. protothecoides culture process, and can also be used as a guideline for similar microalgal cultivation systems.
Received 28 January 1999/ Accepted in revised form 27 August 1999 相似文献
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X-W Zhang X-Y Zeng T Sun X Liu D-X Gu 《Journal of industrial microbiology & biotechnology》1999,23(2):103-105
A fusion gene coding human granulocyte-macrophage colony stimulating factor (GM-CSF) and leukemia inhibitory factor (LIF)
cDNAs was inserted into the transfer vector pSXIVVI+ X3 with the control of Syn and XIV promoters. The Sf9 cells (Spodoptera frugiperda) were co-transfected with the recombinant plasmid and TnNPV DNA (Trichoplusia ni nuclear polyhedrosis virus DNA). The fusion
protein recombinant human granulocyte-macrophage colony stimulating factor (GM-CSF) and leukemia inhibitory factor (LIF) could
be synthesized in cells infected with recombinant virus at a level of about 23% of their total cellular protein. Activity
analysis of the fusion protein in infected cells revealed that it exhibited the dual activities of GM-CSF and LIF. Western
blot analysis of the expressed fusion protein in infected larvae showed that the virus-mediated fusion protein, with a molecular
weight of ∼35 kDa, is confirmed with immunoreactivity.
Received 02 December 1998/ Accepted in revised form 07 May 1999 相似文献