昆虫抗菌肽及其基因工程研究进展

昆虫抗菌肽是昆虫免疫后存在于血淋巴中的一类活性肽,具有广谱的抗菌、抗病毒、抑制肿瘤的生物活性,具有很高的应用潜力。本文主要介绍昆虫抗菌肽的类型、生理活性、基因的克隆与表达及转基因工程研究进展。 Insect Antibacterial Peptides and Its Progress in Advance on Genetic Engineering GONG Xia,YUE Guo-wei,SHI Yong-hui Laboratory for Animal Nutrition Research,College of Food Science,Southern Yangtze University,Wuxi 214036,China Abstract:Insect antibacterial peptides produced are kinds of bioactive peptides in hameolymph hen insect has been immunized.These peptides have the wide range of antibacterial,antiviral spectrum and inhibiting growth of tumor cell.The types of antibacterial peptides and its physiological activities,cloning and expression of the genes and transgenetic research are summarized.Its application on medicine and gene engineering are prospected. Key words：insect antibacterial peptides; physiological activity; gene     

细菌(Pseudomonas maltophilia)之绒毛膜促性腺激素(hCG)结合物质之研究

 细菌(Pseudomonas moltophilia)与hCG及LH有特异的亲和力,实验发现,细菌之生长曲线与hCG结合活性成平行关系,96小时达高峰,细菌之培养液中含有可溶性结合蛋白,该蛋白经硫酸铵沉淀(80％饱和度)、Sephadex G-100柱层析、DEAE-纤维素柱0.5mol/L NaCl梯度洗脱,再过Sepharose CL-AB柱,收集之活性部分经SDS电泳测得其分子量为70,000,凝胶层析测Stokes radius为41A,Schiff氏染色未见着色带。     

RORα高表达对二烯丙基二硫抑制人胃癌细胞增殖与迁移侵袭的影响

目的观察高表达RORα对二烯丙基二硫(DADS)抑制人胃癌MGC803细胞增殖、迁移与侵袭的影响。方法集落形成实验与流式细胞术检测细胞增殖与细胞周期;细胞划痕和Transwell实验分别检测细胞迁移与侵袭。RT-PCR与Western blot分别检测RORα、MMP-9和TIMP3 mRNA与蛋白表达水平。结果RT-PCR与Western blot检测显示,RORα高表达与DADS处理较对照组与空载体组RORαmRNA与蛋白表达明显上调,DADS+RORα高表达组上调更为显著(P<0.05)。与对照组和空载体组比较,RORα高表达与DADS处理组MMP-9表达下调,TIMP3表达上调,DADS+RORα高表达组改变最为显著。集落形成实验显示,RORα高表达与DADS处理组较对照组与空载体组的集落形成率明显降低。流式细胞术显示,与对照组和空载体组比较,RORα高表达与DADS处理组G2/M期细胞比率明显升高。细胞划痕和Transwell实验显示,RORα高表达与DADS处理组细胞迁移与侵袭能力明显降低。结论RORα高表达可通过上调TIMP3与下调MMP-9促进DADS阻滞MGC803细胞G2/M期和抑制增殖与迁移侵袭。     

Absence of estrogen receptor beta leads to abnormal adipogenesis during early tendon healing by an up‐regulation of PPARγ signalling

Achilles tendon injury is one of the challenges of sports medicine, the aetiology of which remains unknown. For a long time, estrogen receptor β (ERβ) has been known as a regulating factor of the metabolism in many connective tissues, such as bone, muscle and cartilage, but little is known about its role in tendon. Recent studies have implicated ERβ as involved in the process of tendon healing. Tendon‐derived stem cells (TDSCs) are getting more and more attention in tendon physiological and pathological process. In this study, we investigated how ERβ played a role in Achilles tendon healing. Achilles tendon injury model was established to analyse how ERβ affected on healing process in vivo. Cell proliferation assay, Western blots, qRT‐PCR and immunocytochemistry were performed to investigate the effect of ERβ on TDSCs. Here, we showed that ERβ deletion in mice resulted in inferior gross appearance, histological scores and, most importantly, increased accumulation of adipocytes during the early tendon healing which involved activation of peroxisome proliferator‐activated receptor γ (PPARγ) signalling. Furthermore, in vitro results of ours confirmed that the abnormity might be the result of abnormal TDSC adipogenic differentiation which could be partially reversed by the treatment of ERβ agonist LY3201. These data revealed a role of ERβ in Achilles tendon healing for the first time, thereby providing a new target for clinical treatment of Achilles tendon injury.     

玉米根冠脱落细胞中微丝分布的荧光显微观察(简报)

在对玉米等主要农作物进行病理及致病毒素毒理分析时,经常选用根冠脱落细胞作为试材。许多学眷认为这些脱落的细胞处于垂死状态,然而,后来的一些研究肯定了Knudson的脱落根冠细胞具有活力的观点。Copoorali无菌培养玉米根冠脱落细胞,发现它们可以分裂;Vermeer等提出存活于玉米根际周围的根冠脱落细胞是根系统范围的扩展,它们在土壤中的作用应予以特别注意;Hawes不仅认为它们为活细胞,而且以它们做实验材料用于毒理分析;Guinal对玉米根冠脱落细胞的来源、细胞结构及生理特性进行了详细研究,肯定了细胞     

狭叶柴胡的抗过敏活性及其有效成分

本文以compound 48／80在体外诱导大鼠腹腔肥大细胞释放组胺,评价狭叶柴胡及其成分的抗过敏活性。狭叶柴胡醇提物及正丁醇部分(100μg／mL)、乙酸乙酯及水部分(10,100μg／mL)显著减少组胺释放,以乙酸乙酯部分的作用最强,狭叶柴胡挥发油(100μg／mL)对组胺释放呈现一定的促进作用。从乙酸乙酯部分分离得到异鼠李素和槲皮素两种主要化合物,均显著抑制组胺释放,表明黄酮为狭叶柴胡抗过敏作用的有效成分。     

Physiological characteristics of the primitive CO2 concentrating mechanism in PEPC transgenic rice

C4 plants such as maize have CO2 concentrating mechanism and higher photosynthetic efficiency than C3 plants, especially under high light intensity, high temperature and drought conditions. In recent years, due to the rapid development of transgenic technique, different transgenic rice plants with high-level expression of C4 genes have been created by the successful introduction of genes encoding the key C4 photosynthetic path enzymes PEPC, PPDK and NADP-ME through agrobacteria-mediated…     

A peptide mimetic of Gal-alpha 1,3-Gal is able to block human natural antibodies

The carbohydrate of Gal-alpha1,3-Gal is thought to be the major antigenic epitope present on pig vascular endothelium. The peptides that mimic the binding of antigenic epitope (Gal-alpha1,3-Gal) to lectin BS-I-B4 were identified from screening a filamentous phage-displayed random library. A phage bearing the peptide NCVSPYWCEPLAPSARA has been identified to bind the lectin strongly. Melibiose was able to inhibit the binding of the human natural anti-alpha Gal antibody to the peptide competitively. Our experiments show that the peptide mimetic of Gal-alpha1,3-Gal is able to inhibit the agglutination of pig RBCs by human natural antibody or lectin BS-I-B4. The peptide inhibitor of human natural antibodies may prove useful in pig-to-human xenotransplantation.     

Duvira Antarctic polysaccharide inhibited H1N1 influenza virus-induced apoptosis through ROS mediated ERK and STAT-3 signaling pathway

Background

The H1N1 influenza virus causes acute respiratory tract infection, and its clinical symptoms are very similar to those of ordinary influenza. The disease develops rapidly. If the flu is not treated, complications such as pneumonia, respiratory failure, and multiple organ damage can occur, resulting in a high fatality rate. Influenza virus mutates rapidly. At present, there is no specific drug for H1N1, so it is an urgent need for clinical care to find new drugs to treat H1N1.

Materials and methods

The polysaccharide derived from Durvillaea Antarctica green algae has a certain antiviral effect. In this study, the results of CCK-8, apoptosis cycle detection, JC-1 and Western blotting proved that Duvira Antarctic polysaccharide (DAPP) has the ability to inhibit H1N1 infection.

Results

CCK-8 test showed that the DAPP with concentration at 32 μg/mL had no toxicity to MDCK cells. In addition, DAPP reduced cell apoptosis by inhibiting the ERK signaling pathway. Meanwhile, DAPP could increase the expression of STAT3 and significantly inhibited proinflammatory cytokines.

Conclusions

In summary, these results suggested that DAPP may be potential with the ability to resist the H1N1 influenza virus.