全文获取类型
收费全文 | 66618篇 |
免费 | 5684篇 |
国内免费 | 6082篇 |
出版年
2024年 | 113篇 |
2023年 | 767篇 |
2022年 | 1478篇 |
2021年 | 3299篇 |
2020年 | 2209篇 |
2019年 | 2750篇 |
2018年 | 2548篇 |
2017年 | 1940篇 |
2016年 | 2713篇 |
2015年 | 4066篇 |
2014年 | 4847篇 |
2013年 | 5212篇 |
2012年 | 6162篇 |
2011年 | 5662篇 |
2010年 | 3453篇 |
2009年 | 3216篇 |
2008年 | 3629篇 |
2007年 | 3263篇 |
2006年 | 2778篇 |
2005年 | 2479篇 |
2004年 | 2046篇 |
2003年 | 1807篇 |
2002年 | 1531篇 |
2001年 | 1274篇 |
2000年 | 1084篇 |
1999年 | 1071篇 |
1998年 | 672篇 |
1997年 | 651篇 |
1996年 | 620篇 |
1995年 | 533篇 |
1994年 | 502篇 |
1993年 | 392篇 |
1992年 | 530篇 |
1991年 | 464篇 |
1990年 | 397篇 |
1989年 | 306篇 |
1988年 | 286篇 |
1987年 | 233篇 |
1986年 | 172篇 |
1985年 | 227篇 |
1984年 | 133篇 |
1983年 | 118篇 |
1982年 | 92篇 |
1981年 | 70篇 |
1980年 | 57篇 |
1979年 | 74篇 |
1978年 | 66篇 |
1977年 | 48篇 |
1976年 | 49篇 |
1973年 | 48篇 |
排序方式: 共有10000条查询结果,搜索用时 500 毫秒
71.
72.
73.
X. Y. Li C. M. Han Y. Wang H. Z. Liu Z. F. Wu Q. H. Gao S. H. Zhao 《Animal genetics》2010,41(5):537-540
RIG-1 signalling is responsible for the detection of cytoplasmic viral RNA molecules. DEXH (Asp-Glu-X-His) box polypeptide 58 (encoded by DHX58) is a negative regulator of the RIG-1 signalling pathway. In human, the DHX58 gene can be upregulated and can inhibit the RIG-1 signalling pathway during viral infection. In this study, porcine DHX58 gene expression patterns were studied. According to our results, the porcine DHX58 gene was upregulated not only by the stimulation of Poly I:C but also by the stimulation of 1ipopolysaccharides (LPS). One polymorphism (g.4919G>C), detected in the ninth intron, was significantly associated with some blood parameters including the red cell distribution width of 1-day-old pigs and white blood cell counts, lymphocyte absolute counts, and platelet distribution width of 17-day-old pigs (P < 0.05). Moreover, the individuals with the genotype GG have a significantly higher mean white blood cell count than individuals with genotype CC or GC (P < 0.05). Our study indicates that DHX58 is an important gene that is associated with the immune response in swine. 相似文献
74.
75.
76.
Yan-Hong He Guo-Gui Ning Ya-Lin Sun Yan Hu Xing-Yu Zhao Man-Zhu Bao 《Molecular breeding : new strategies in plant improvement》2010,26(1):19-29
A male sterile line was isolated in marigold (Tagetes erecta L.) and cytological analysis determined this to be a novel genic male sterility trait (Tems). Through the use of amplified fragment length polymorphisms (AFLPs) and bulked segregant analysis (BSA), tightly linked
markers of Tems were identified with a view towards a map-based cloning strategy. It was found that spontaneous homeotic conversion of floral
organs was the underlying cause of the male sterility in this marigold line. Thus, petals of male sterile plants resembled
sepal-like structures and the stamens were partially converted to styles, although without the full characteristics or function
of the true style organs. We have constructed a fine marker-based map for the Tems gene. This is intended to provide a tool for marker assisted selection (MAS) strategies in hybrid breeding and map-based
cloning strategies for the male sterility locus. We discuss the significance of this spontaneously derived genic male sterility
trait relating to the homeotic conversion of floral organs in marigold. 相似文献
77.
Inhibitors of ADP-ribosylation inhibited the myeloid differentiation of murine myelomonocytic leukemia, WEHI-3BD+ cells induced by granulocyte colony-stimulating factor. Benzamide, at 2.0 mM, inhibited 50% of the WEHI-3BD+ cell differentiation but had no significant effect on the proliferation. However, benzylaminododecylguanine hydrochloride and p-methoxylbenzylaminodecamethylene guanidine sulfate at 2.0 and 2.2 microM, respectively, inhibited 50% of proliferation but had no effect at all on differentiation. The differential effects of inhibitors provide a model to study the role of ADP-ribosylation in myeloid differentiation. 相似文献
78.
The addition of 0.167 to 4.0 mM cAMP to gel-filtered rabbit reticulocyte lysates stimulates the initial rate and the extent of polypeptide synthesis. The stimulation is at the initiation step of polypeptide synthesis as measured by the (i) increased dipeptide, methionyl-valine, accumulation in the presence of the specific initiation inhibitor, pactamycin, and (ii) increased formation of the 40 S and 80 S initiation complex when gel-filtered lysates are incubated with [35S]Met-tRNAFMet. Furthermore, a synergistic stimulation of protein synthesis is observed when cAMP and hexose phosphates (which alone elicit a 1.8-fold stimulation of protein synthesis) are added simultaneously to gel-filtered rabbit reticulocyte lysates. These results indicate that cAMP and hexose phosphates are both essential to maintain the high rate of initiation. 相似文献
79.
80.