全文获取类型
收费全文 | 6689篇 |
免费 | 789篇 |
国内免费 | 5篇 |
专业分类
7483篇 |
出版年
2021年 | 75篇 |
2019年 | 82篇 |
2018年 | 71篇 |
2017年 | 59篇 |
2016年 | 109篇 |
2015年 | 164篇 |
2014年 | 191篇 |
2013年 | 231篇 |
2012年 | 336篇 |
2011年 | 295篇 |
2010年 | 166篇 |
2009年 | 174篇 |
2008年 | 226篇 |
2007年 | 236篇 |
2006年 | 220篇 |
2005年 | 234篇 |
2004年 | 214篇 |
2003年 | 227篇 |
2002年 | 226篇 |
2001年 | 195篇 |
2000年 | 214篇 |
1999年 | 153篇 |
1998年 | 93篇 |
1997年 | 72篇 |
1996年 | 81篇 |
1995年 | 81篇 |
1994年 | 68篇 |
1993年 | 77篇 |
1992年 | 152篇 |
1991年 | 136篇 |
1990年 | 138篇 |
1989年 | 167篇 |
1988年 | 131篇 |
1987年 | 125篇 |
1986年 | 100篇 |
1985年 | 118篇 |
1984年 | 117篇 |
1983年 | 95篇 |
1982年 | 79篇 |
1981年 | 76篇 |
1980年 | 77篇 |
1979年 | 109篇 |
1978年 | 90篇 |
1977年 | 63篇 |
1976年 | 71篇 |
1975年 | 70篇 |
1974年 | 81篇 |
1973年 | 80篇 |
1972年 | 56篇 |
1971年 | 53篇 |
排序方式: 共有7483条查询结果,搜索用时 0 毫秒
941.
Dieuwke B. van Dorp Alan F. Wright Andrew D. Carothers Elizabeth M. Bleeker-Wagemakers 《Human genetics》1992,88(3):331-334
Summary The results of linkage analysis in a family with X-linked retinitis pigmentosa (XLRP) are presented. Probe M27B (DXS255), localised to Xp11.22, was only loosely linked to XLRP, whereas pHOC3 (OTC), in the more distal Xp21.1 region, was tightly linked. In this family, the conditional probability of an RP3 locus (in Xp21.1–p11.4) was found to be 0.978 compared with 0.021 for an RP2 locus (in Xp11.4–p11.2). Risk assessment showed that 2 out of 4 at risk females showing no clinical abnormality have a high probability of being genetic carriers of XLRP. Some affected males have recurrent respiratory infections as a result of a condition indistinguishable from the immotile cilia syndrome; indeed, there is an association between XLRP and susceptibility to respiratory infections in the majority of affected males. The possibility that previously observed ciliary abnormalities in XLRP patients might be associated specifically with an RP3 locus abnormality is discussed. 相似文献
942.
943.
944.
945.
946.
C. Wright G. Herbert R. Pilkington M. Callaghan S. McClean 《Letters in applied microbiology》2010,50(5):500-506
Aims: To develop a rapid method to quantify the attachment of the cystic fibrosis pathogen, Burkholderia multivorans, to lung epithelial cells (16HBE14o?) using real‐time PCR with a view to monitoring potential inhibition of lung cell attachment. Methods and Results: Mammalian and bacterial DNA were purified from bacteria attached to lung epithelial cells. The relative amount of bacteria attached was determined by amplification of the recA gene relative to the human GAPDH gene, in the presence of SYBR Green®. The method was thoroughly validated and shown to correlate well with traditional plating techniques. Inhibition of bacterial attachment with simple sugars was then evaluated by real‐time PCR. Of the sugars examined, pre‐incubation of B. multivorans with lactose, mannose and xylitol all decreased bacterial adherence to 16HBE14o? cells, while glucose and galactose had no significant effect. Pre‐incubation with lactose had the greatest effect, resulting in reduced adhesion to 35% of untreated controls. Conclusions: This method can be used to quickly and effectively screen novel agents with higher affinities for bacterial adhesins. Significance and Impact of the Study: This method will enable the rapid development of novel agents to inhibit colonization by this pathogen from the environment. 相似文献
947.
An in vitro binding assay involving egg plasma membrane vesicles (PMVs) of Fucus serratus L. and proteins contained in a KCl extract of sperm has been used to identify a sperm protein involved in egg binding. High-performance gel filtration (HPGF) separated the sperm KCl extract into several major fractions, and a protein (apparent M, 60 kDa) was identified as being involved in binding to the egg PMVs. This protein ran on denaturing sodium dodecyl sulfate (SDS)gels with an apparent molecular weight of 27 kDa. This suggests that either the native form of the protein is a dimer or the molecular weight on HPGF is an artifact caused by high ionic strength buffer promoting hydrophobic interactions. When KCl-sol-uble proteins were separated by SDS-polyacrylamide gel electrophoresis (PAGE), blotted onto nitrocellulose, and incubated with biotinylated egg PMVs, these bound to a band at 27 kDa, confirming the role of this protein. Addition of the Fucus sperm extract or HPGF fractions containing the binding protein to eggs in the absence of sperm induced the release of polysaccharides onto the egg cell surface. This labeling was patchy, in contrast to the uniform release of polysaccharides observed when sperm were added to eggs. The monoclonal antibody (MAb) FS17 was raised against the 27-kDa sperm protein. It labeled the sperm body and both flagella by immunofluorescence, though the sperm had to he permeabilized to observe labeling, suggesting that the epitope recognized is not exposed at the cell surface. Addition of FS17 to the KCl extract in the binding assay reduced subsequent binding of egg PMVs. Removal of the 27-kDa protein recognized by FS17 from the sperm extract prevented the binding of egg PMVs in the binding assay and the triggering of the patchy release of polysaccharides when added to eggs. Overall the results suggest that the 27-kDa sperm protein is involved in binding to the egg plasma membrane and can trigger partial activation of the egg . 相似文献
948.
This paper defines a collection of Drosophila deletion mutations (deficiencies) that can be systematically screened for embryonic phenotypes, orphan receptor ligands, and genes affecting protein localization. It reports the results of deficiency screens we have conducted that have revealed new axon guidance phenotypes in the central nervous system and neuromuscular system and permitted a quantitative assessment of the number of potential genes involved in regulating guidance of specific motor axon branches. Deficiency “kits” that cover the genome with a minimum number of lines have been established to facilitate gene mapping. These kits cannot be systematically analyzed for phenotypes, however, since embryos homozygous for many deficiencies in these kits fail to develop due to the loss of key gene products encoded within the deficiency. To create new kits that can be screened for phenotype, we have examined the development of the nervous system in embryos homozygous for more than 700 distinct deficiency mutations. A kit of ∼400 deficiency lines for which homozygotes have a recognizable nervous system and intact body walls encompasses >80% of the genome. Here we show examples of screens of this kit for orphan receptor ligands and neuronal antigen expression. It can also be used to find genes involved in expression, patterning, and subcellular localization of any protein that can be visualized by antibody staining. A subset kit of 233 deficiency lines, for which homozygotes develop relatively normally to late stage 16, covers ∼50% of the genome. We have screened it for axon guidance phenotypes, and we present examples of new phenotypes we have identified. The subset kit can be used to screen for phenotypes affecting all embryonic organs. In the future, these deficiency kits will allow Drosophila researchers to rapidly and efficiently execute genome-wide anatomical screens that require examination of individual embryos at high magnification. 相似文献
949.
950.