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201.
Hendrik Schubert Hans C.P. Matthijs Luuc R. Mur Ulrich Schiewer 《FEMS microbiology ecology》1995,18(3):237-245
Abstract: The influence of intermittent high-light dosage on Synechocystis sp. PCC 6803 with respect to oxygen evolution capacity, fluorescence yield and carotenoid pigment pattern was investigated, using high-light- and low-light-adapted cultures. The results showed that this cyanobacterium was able to survive high light stress for a full day if this stress was applied on and off with intermittently presented recovery periods in darkness. Enhanced respiratory activity in the high-light adapted cells was detected and this may be an important factor in preventing photodamage under high light stress. Cyanobacterial photosynthetic and respiratory electron transfer pathways are both present within the same membrane, and share common electron carriers. The role of respiratory activity in preventing overexcitation of photosystem 2 is discussed with regard to cyanobacterial ecology. 相似文献
202.
Wisselink HW Toirkens MJ del Rosario Franco Berriel M Winkler AA van Dijken JP Pronk JT van Maris AJ 《Applied and environmental microbiology》2007,73(15):4881-4891
For cost-effective and efficient ethanol production from lignocellulosic fractions of plant biomass, the conversion of not only major constituents, such as glucose and xylose, but also less predominant sugars, such as l-arabinose, is required. Wild-type strains of Saccharomyces cerevisiae, the organism used in industrial ethanol production, cannot ferment xylose and arabinose. Although metabolic and evolutionary engineering has enabled the efficient alcoholic fermentation of xylose under anaerobic conditions, the conversion of l-arabinose into ethanol by engineered S. cerevisiae strains has previously been demonstrated only under oxygen-limited conditions. This study reports the first case of fast and efficient anaerobic alcoholic fermentation of l-arabinose by an engineered S. cerevisiae strain. This fermentation was achieved by combining the expression of the structural genes for the l-arabinose utilization pathway of Lactobacillus plantarum, the overexpression of the S. cerevisiae genes encoding the enzymes of the nonoxidative pentose phosphate pathway, and extensive evolutionary engineering. The resulting S. cerevisiae strain exhibited high rates of arabinose consumption (0.70 g h(-1) g [dry weight](-1)) and ethanol production (0.29 g h(-1) g [dry weight](-1)) and a high ethanol yield (0.43 g g(-1)) during anaerobic growth on l-arabinose as the sole carbon source. In addition, efficient ethanol production from sugar mixtures containing glucose and arabinose, which is crucial for application in industrial ethanol production, was achieved. 相似文献
203.
Velamakanni S Wei SL Janvilisri T van Veen HW 《Journal of bioenergetics and biomembranes》2007,39(5-6):465-471
The ATP-binding cassette (ABC) transporter superfamily is one of the largest protein families with representatives in all
kingdoms of life. Members of this superfamily are involved in a wide variety of transport processes with substrates ranging
from small ions to relatively large polypeptides and polysaccharides. The G subfamily of ABC transporters consists of half-transporters,
which oligomerise to form the functional transporter. While ABCG1, ABCG4 and ABCG5/8 are involved in the ATP-dependent translocation
of steroids and, possibly, other lipids, ABCG2 (also termed the breast cancer resistance protein) has been identified as a
multidrug transporter that confers resistance on tumor cells. Evidence will be summarized suggesting that ABCG2 can also mediate
the binding/transport of non-drug substrates, including free and conjugated steroids. The characterization of the substrate
specificities of ABCG proteins at a molecular level might provide further clues about their potential physiological role(s),
and create new opportunities for the modulation of their activities in relation to human disease. 相似文献
204.
No evidence for substantial aerobic methane emission by terrestrial plants: a 13C-labelling approach 总被引:2,自引:0,他引:2
Dueck TA de Visser R Poorter H Persijn S Gorissen A de Visser W Schapendonk A Verhagen J Snel J Harren FJ Ngai AK Verstappen F Bouwmeester H Voesenek LA van der Werf A 《The New phytologist》2007,175(1):29-35
* The results of a single publication stating that terrestrial plants emit methane has sparked a discussion in several scientific journals, but an independent test has not yet been performed. * Here it is shown, with the use of the stable isotope (13)C and a laser-based measuring technique, that there is no evidence for substantial aerobic methane emission by terrestrial plants, maximally 0.3% (0.4 ng g(-1) h(-1)) of the previously published values. * Data presented here indicate that the contribution of terrestrial plants to global methane emission is very small at best. * Therefore, a revision of carbon sequestration accounting practices based on the earlier reported contribution of methane from terrestrial vegetation is redundant. 相似文献
205.
Two recently collected slabs from the Lower Devonian Hunsrück Slate of Bundenbach, Hunsrück region, Germany, with spines of
the acanthodianMachaeracanthus hunsrueckianum n. sp. are described. Both are associations of large and small spines and are the first to show groupings of symmetrical
pairs; the spines are not homologous with those of other acanthodians. A pair of small spines ofMachaeracanthus peracutus
Newberry, 1857 from the Karschheck quarry near Oberkirn, Hunsrück region, Germany, is articulated with the pectoral girdle and is
the first such complex to be described. The only spines whichMachaeracanthus appears to have had were a pair of large and small pectoral spines on each side of the body. These spines could have helped
to prevent the fish from sinking into the mud while resting on the sea floor. 相似文献
206.
Ethylene insensitivity results in down-regulation of rubisco expression and photosynthetic capacity in tobacco 总被引:2,自引:0,他引:2
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Little is known about the effect of hormones on the photosynthetic process. Therefore, we studied Rubisco content and expression along with gas exchange parameters in transgenic tobacco (Nicotiana tabacum) plants that are not able to sense ethylene. We also tested for a possible interaction between ethylene insensitivity, abscisic acid (ABA), and sugar feedback on photosynthesis. We measured Rubisco content in seedlings grown in agar with or without added sugar and fluridone, and Rubisco expression in hydroponically grown vegetative plants grown at low and high CO(2). Furthermore, we analyzed gas exchange and the photosynthetic machinery of transformants and wild-type plants grown under standard conditions. In the presence of exogenous glucose (Glc), agar-grown seedlings of the ethylene-insensitive genotype had lower amounts of Rubisco per unit leaf area than the wild type. No differences in Rubisco content were found between ethylene-insensitive and wild-type seedlings treated with fluridone, suggesting that inhibition of ABA production nullified the effect of Glc application. When larger, vegetative plants were grown at different atmospheric CO(2) concentrations, a negative correlation was found between Glc concentration in the leaves and Rubisco gene expression, with stronger repression by high Glc concentrations in ethylene-insensitive plants. Ethylene insensitivity resulted in plants with comparable fractions of nitrogen invested in light harvesting, but lower amounts in electron transport and Rubisco. Consequently, photosynthetic capacity of the insensitive genotype was clearly lower compared with the wild type. We conclude that the inability to perceive ethylene results in increased sensitivity to Glc, which may be mediated by a higher ABA concentration. This increased sensitivity to endogenous Glc has negative consequences for Rubisco content and photosynthetic capacity of these plants. 相似文献
207.
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209.
There is now evidence that chronic fatigue syndrome (CFS) is accompanied by an increased translocation of endotoxins from gram-negative enterobacteria through the gut wall, as demonstrated by increased prevalences and median values for serum IgM and IgA against the endotoxins of gram-negative enterobacteria. This condition can also be described as increased gut permeability or leaky gut and indicates intestinal mucosal dysfunction (IMD). Here we report a case of a 13 year old girl with CFS who showed very high values for serum IgM against the LPS of some enterobacteria and signs of oxidative and nitrosative stress, activation of the inflammatory response system, and IgG3 subclass deficiency. Upon treatment with specific antioxidants and a "leaky gut diet", which both aim to treat increased gut permeability, and immunoglobins intravenously, the increased translocation of the LPS of gram negative enterobacteria normalized and this normalization was accompanied by a complete remission of the CFS symptoms. 相似文献