全文获取类型
收费全文 | 4387篇 |
免费 | 297篇 |
国内免费 | 4篇 |
专业分类
4688篇 |
出版年
2024年 | 3篇 |
2023年 | 18篇 |
2022年 | 64篇 |
2021年 | 82篇 |
2020年 | 68篇 |
2019年 | 87篇 |
2018年 | 126篇 |
2017年 | 112篇 |
2016年 | 179篇 |
2015年 | 295篇 |
2014年 | 298篇 |
2013年 | 347篇 |
2012年 | 375篇 |
2011年 | 371篇 |
2010年 | 246篇 |
2009年 | 222篇 |
2008年 | 267篇 |
2007年 | 259篇 |
2006年 | 228篇 |
2005年 | 222篇 |
2004年 | 199篇 |
2003年 | 165篇 |
2002年 | 162篇 |
2001年 | 60篇 |
2000年 | 43篇 |
1999年 | 31篇 |
1998年 | 32篇 |
1997年 | 16篇 |
1996年 | 20篇 |
1995年 | 9篇 |
1994年 | 11篇 |
1993年 | 7篇 |
1992年 | 6篇 |
1991年 | 3篇 |
1990年 | 5篇 |
1989年 | 9篇 |
1988年 | 3篇 |
1987年 | 4篇 |
1986年 | 3篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1979年 | 4篇 |
1977年 | 3篇 |
1976年 | 2篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1966年 | 2篇 |
1961年 | 1篇 |
排序方式: 共有4688条查询结果,搜索用时 0 毫秒
41.
Mi Hye Kim Jae Yeop Kim Jung‐Hak Kim Hyun‐Shik Lee Jae‐Won Huh Dong‐Seok Lee 《Cell biology international》2020,44(10):2086-2093
Reactive oxygen species (ROS) act as signaling molecules to regulate various cell functions. Numerous studies have demonstrated ROS to be essential for the differentiation of adipocytes. Peroxiredoxins (Prxs) are a ubiquitous family of antioxidant enzymes in mammalian cells. Prx2 is present in the cytoplasm and cell membranes and demonstrates ROS scavenging activity. We focused on Prx2 involvement in regulating adipogenesis and lipid accumulation and demonstrated that Prx2 expression was upregulated during adipocyte differentiation. In addition, the silencing of Prx2 (shPrx2) inhibited adipogenesis by modulating adipogenic gene expression, and cell death was enhanced via increased ROS production in shPrx2‐3T3‐L1 cells. These results demonstrate that shPrx2 triggers adipocyte cell death and weakens adipocyte function via ROS production. Taken together, our data suggest the participation of Prx2 in adipocyte function and differentiation. Our results also imply that the downregulation of Prx2 activity could help prevent obesity. Overall, findings support the development of ROS‐based therapeutic solutions for the treatment of obesity and obesity‐related metabolic disorders. 相似文献
42.
Ilya A. Udalov Won Je Lee Kirill Lotonin Alexey Smirnov 《The Journal of eukaryotic microbiology》2020,67(1):132-139
A new marine species of naked lobose amoebae Pseudoparamoeba garorimi n. sp. (Amoebozoa, Dactylopodida) isolated from intertidal marine sediments of Garorim Bay, Korea was studied with light and transmission electron microscopy. This species has a typical set of morphological characters for a genus including the shape of the locomotive form, type of subpseudopodia and the tendency to form the single long waving pseudopodium in locomotion. Furthermore, it has the same cell surface structures as were described for the type species, Pseudoparamoeba pagei: blister‐like glycostyles with hexagonal base and dome‐shaped apex; besides, cell surface bears hair‐like outgrowths. The new species described here lacks clear morphological distinctions from the two other Pseudoparamoeba species, but has considerable differences in the 18S rDNA and COX1 gene sequences. Phylogenetic analysis based on 18S rDNA placed P. garorimi n. sp. at the base of the Pseudoparamoeba clade with high PP/BS support. The level of COX1 sequence divergence was 22% between P. garorimi n. sp. and P. pagei and 25% between P. garorimi n. sp. and P. microlepis. Pseudoparamoeba species are hardly distinguishable by morphology alone, but display clear differences in 18S rDNA and COX1 gene sequences. 相似文献
43.
Woo Yong Sung Ji Won Yu Jong Tae Hwang Hee Jin Nam Ji Ye Park Yongae Kim Jang‐Hee Cho 《Journal of peptide science》2020,26(8)
Antimicrobial peptides are class of small, positively charged peptides known for their broad‐spectrum antimicrobial activity. Antimicrobial activities for most antimicrobial peptides have largely remained elusive, particularly in the lactic acid bacteria. However, recently our investigation using LPcin‐YK3, an antimicrobial peptide from bovine milk, suggests that in vitro antimicrobial activity was reduced over 100‐fold compared with pathogenic bacteria. Additionally, for the structural study of how antimicrobial peptide undergoes its reaction at the proteolytic pathway of lactic acid bacteria based on degradation assay and propidium iodide staining, we performed molecular docking for interaction between oligopeptide‐binding protein A and LPcin‐YK3 peptide. Given that degradation related to the LPcin‐YK3 peptide in lactic acid bacteria proteolytic system, the inhibitory inactivity of LPcin‐YK3 against beneficial lactic acid bacteria strains may be one of the primary pharmacological properties of recombinant peptide discovered in bovine milk. These results provide structural and functional insights into the proteolytic mechanism and possibility as a putative substrate of oligopeptide‐binding protein A in respect of LPcin‐YK3 peptide. 相似文献
44.
The hypothesis that environmental heterogeneity promotes species richness by increasing opportunities for niche partitioning is a fundamental paradigm in ecology. However, recent studies suggest that heterogeneity–diversity relationships (HDR) are more complex than expected from this niche‐based perspective, and often show a decrease in richness at high levels of heterogeneity. These findings have motivated ecologists to propose new mechanisms that may explain such deviations. Here we provide an overview of currently recognised mechanisms affecting the shape of HDRs and present a conceptual model that integrates all previously proposed mechanisms within a unified framework. We also translate the proposed framework into an explicit community dynamic model and use the model as a tool for generating testable predictions concerning how landscape properties interact with species traits in determining the shape of HDRs. Our main finding is that, despite the enormous complexity of such interactions, the predicted HDRs are rather simple, ranging from positive to unimodal patterns in a highly consistent and predictable manner. 相似文献
45.
Lee Seokjin Trịnh Cao Sơn Lee Won Je Jeong Chan Young Truong Hai An Chung Namhyun Kang Chon-Sik Lee Hojoung 《Journal of plant research》2020,133(2):231-244
Journal of Plant Research - Plant growth promoting rhizobacteria (PGPR) are a group of bacteria that promote plants growth in the rhizosphere. PGPRs are involved in various mechanisms that... 相似文献
46.
47.
48.
49.
50.