全文获取类型
收费全文 | 693849篇 |
免费 | 75446篇 |
国内免费 | 365篇 |
专业分类
769660篇 |
出版年
2016年 | 7616篇 |
2015年 | 10081篇 |
2014年 | 11972篇 |
2013年 | 17907篇 |
2012年 | 20443篇 |
2011年 | 21187篇 |
2010年 | 13969篇 |
2009年 | 12552篇 |
2008年 | 18850篇 |
2007年 | 19778篇 |
2006年 | 18645篇 |
2005年 | 17855篇 |
2004年 | 17655篇 |
2003年 | 17433篇 |
2002年 | 16958篇 |
2001年 | 26913篇 |
2000年 | 27081篇 |
1999年 | 21657篇 |
1998年 | 8158篇 |
1997年 | 8557篇 |
1996年 | 8368篇 |
1995年 | 7779篇 |
1994年 | 7701篇 |
1993年 | 7698篇 |
1992年 | 18967篇 |
1991年 | 18550篇 |
1990年 | 18406篇 |
1989年 | 18107篇 |
1988年 | 17002篇 |
1987年 | 16398篇 |
1986年 | 15282篇 |
1985年 | 15878篇 |
1984年 | 13363篇 |
1983年 | 11660篇 |
1982年 | 9186篇 |
1981年 | 8471篇 |
1980年 | 7860篇 |
1979年 | 13110篇 |
1978年 | 10291篇 |
1977年 | 9680篇 |
1976年 | 9356篇 |
1975年 | 10133篇 |
1974年 | 11053篇 |
1973年 | 10820篇 |
1972年 | 10014篇 |
1971年 | 9087篇 |
1970年 | 7925篇 |
1969年 | 7927篇 |
1968年 | 7314篇 |
1967年 | 6281篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
L Galeazzi G Turchetti G Grilli G Groppa S Giunta 《Applied and environmental microbiology》1986,52(6):1433-1435
Chlorpromazine was used to perform a test for the detection of microbial peroxidase activities. The compound acts as both a cell permeabilizer and a reagent in the procedure developed which allows the detection of peroxidase and peroxidase like reactions both semiquantitatively in whole cell determinations and quantitatively in cell-free supernatants. 相似文献
992.
V L Ganelin A A Denisov G L Shaposhnikov Iu O Sazykin S M Navashin 《Biokhimii?a (Moscow, Russia)》1980,45(3):483-491
Aminoglycoside-3'-phosphotransferase I and II (APT-3'-I and APT-3'-II) has been purified to homogenity from the cells of E. coli containing the plasmids R6 and JR67, respectively. The purification procedure involved competitive affinity chromatography on neomycin-sepharose and gel-filtration on Sephadex G-100. The specific activity of APT-3'-I with the substrates--lividomycin A, neomycin B, paromycin, ribostamycin, kanamycins A and B--are 4.3, 2.8, 2.1, 1.6, 0.9 and 0.8 mol/min. mg protein, respectively. The specific activity of APT-3'-II with the substrates--ribostamycin, paromycin, kanamycins A and B, neomycin B--are 8.0, 7.2, 4.0, 4.5 and 3.6, respectively. Mg2+ is required for the activity of both enzymes. Co2+, Zn2+ and Mn2+ are active in case of APT-3'-I; however, these cations are less active than Mg2+. The pH-optimum of APT-3'-I and APT-3'-II is 7.0--7.5. High ionic strength is required for the activity of both enzymes. The molecular weights of APT-3'-I and APT-3'-II are about 36 000 and 26 000, respectively. The amino acid composition of APT-3'-I and APT-3'-II was determined. Both enzymes contain tryptophane residues whose fluorescence intensity decreased when ATP, but not amino-glycoside antibiotics, is added. The interrelationship between the molecular weights of these enzymes and the sizes of the loops of transposones Tn 601 and Tn 5, encoding APT-3'-I and APT-3'-II, is discussed. 相似文献
993.
I. J. Pickering Graham N. George Verena Van Fleet-Stalder Thomas G. Chasteen Roger C. Prince 《Journal of biological inorganic chemistry》1999,4(6):791-794
Received: 2 April 1999 / Accepted: 17 September 1999 相似文献
994.
995.
SZNAJDER W 《Polski tygodnik lekarski (Warsaw, Poland : 1960)》1952,7(42):1325-1329
996.
M A Chebotareva L P Grokhovski? T G David'ian 《Zhurnal evoliutsionno? biokhimii i fiziologii》1987,23(5):588-592
Studies have been made on the lipid composition of total lipids, triglycerides and their fatty acids, cholesterol and phospholipids in the vertebral column of young and adult rabbits. It was shown that the content of total lipids and triglycerides increases, whereas that of cholesterol and phospholipids decreases with age. The content of total lipids in the vertebral column is 10 times higher as compared to that in the bones of the extremities. Mid-thoracic part of the vertebral column exhibits higher lipid content than other thoracic parts of the column. Lipid content of the vertebral processes is lower than that of the vertebral bodies. These data indicate lipid specificity and heterogeneity of bone tissue of the vertebral column. The main fatty acids of vertebral triglycerides are presented by those with 14-18 carbon atoms (90%), no acids with 22 atoms were found. Higher content of the linoleic acid (19%) and higher total unsaturation of triglycerides were found in the bone tissue of rabbits in comparison with those of man. 相似文献
997.
998.
W J Allard C D Cheli D L Morris J Goldblatt Y Pierre L Kish Y Chen J Dai R L Vessella D W Chan M K Schwartz Z Zhou K K Yeung 《The International journal of biological markers》1999,14(2):73-83
We conducted a multicenter evaluation of the analytical and clinical performance of the automated Bayer Immuno 1 complexed PSA (cPSA) assay, and compared assay performance to the Bayer Immuno 1 PSA assay. We sought to determine whether measurements of cPSA could be of clinical utility in the management of patients with prostate cancer. Results of the 10-day imprecision across three evaluation sites produced total CV < 2.50% and an analytical sensitivity of 0.02 microgram/L. There was an increased trend in clinical sensitivity for prostate cancer with increasing stage of disease (71-86%). Clinical specificity for patients with benign urogenital disease was 74.8%, and for other nonprostate diseases ranged from 91.1-100%. Retrospective serial monitoring of 155 patients with prostate cancer demonstrated concordance of cPSA measurements to clinical status for 97% of the patients analyzed. Results from the clinical studies using the Bayer Immuno 1 cPSA assay were comparable to results obtained with the Bayer Immuno 1 PSA assay. The Bayer Immuno 1 cPSA assay demonstrates analytical performance and clinical effectiveness in the management of prostate cancer patients during the course of disease and therapy. 相似文献
999.
To study the role of cell division in the process of nucleoid segregation, we measured the DNA content of individual nucleoids in isogenic Escherichia coli cell division mutants by image cytometry. In pbpB(Ts) and ftsZ strains growing as filaments at 42 degrees C, nucleoids contained, on average, more than two chromosome equivalents compared with 1.6 in wild-type cells. Because similar results were obtained with a pbpB recA strain, the increased DNA content cannot be ascribed to the occurrence of chromosome dimers. From the determination of the amount of DNA per cell and per individual nucleoid after rifampicin inhibition, we estimated the C and D periods (duration of a round of replication and time between termination and cell division respectively), as well as the D' period (time between termination and nucleoid separation). Compared with the parent strain and in contrast to ftsQ, ftsA and ftsZ mutants, pbpB(Ts) cells growing at the permissive temperature (28 degrees C) showed a long D' period (42 min versus 18 min in the parent) indicative of an extended segregation time. The results indicate that a defective cell division protein such as PbpB not only affects the division process but also plays a role in the last stage of DNA segregation. We propose that PbpB is involved in the assembly of the divisome and that this structure enhances nucleoid segregation. 相似文献
1000.
N-Methyl-D-Aspartate receptors in acquisition of the short-term memory in the honeybeeApis mellifera
N. G. Lopatina I. V. Ryzhova E. G. Chesnokova L. A. Dmitrieva 《Journal of Evolutionary Biochemistry and Physiology》2000,36(3):294-300
Role of NMDA receptors in the process of associative learning has been studied in the honeybeeApis mellifera L. in behavior experiments, using method of conditional reflexes. To determine pharmacological profile of NMDA receptors,
effects of Mg2+ ions, NMDA, glycine (Gly), antagonist of the glycine site 5,7-dichlorokynurenic acid (DCK), competitive antagonists of NMDA
receptors: D,L-2-aminophosphovalerate (APV), L-2-aminophosphobutyrate (APB), and D-glutamyl-aminomethylphosphonic acid (GAMP)
as well of antagonists of NMDA receptor ion channels MK-801 and ketamine (Ket) were studied on acquisition of alimentary conditional
reflex and its retention in memory. NMDA increased capacity for learning by stimulating shortterm memory. The NMDA receptor
co-agonist Gly activated this NMDA effect. DCK eliminated the NMDA and Gly stimulatoty effects. All tested antagonists at
millimolar concentrations inhibited associative function. The data obtained confirm our hypothesis about participation of
NMDA receptors in processes of formation of short-term memory in the honeybeeApis mellifera and suggest that functional characteristics of the NMDA receptors involved in the process of associative learning in the
honeybee resemble those in mammals. 相似文献