全文获取类型
收费全文 | 9646篇 |
免费 | 842篇 |
国内免费 | 8篇 |
出版年
2023年 | 45篇 |
2022年 | 86篇 |
2021年 | 191篇 |
2020年 | 133篇 |
2019年 | 144篇 |
2018年 | 232篇 |
2017年 | 183篇 |
2016年 | 282篇 |
2015年 | 470篇 |
2014年 | 461篇 |
2013年 | 578篇 |
2012年 | 773篇 |
2011年 | 677篇 |
2010年 | 449篇 |
2009年 | 355篇 |
2008年 | 531篇 |
2007年 | 493篇 |
2006年 | 491篇 |
2005年 | 472篇 |
2004年 | 469篇 |
2003年 | 439篇 |
2002年 | 356篇 |
2001年 | 139篇 |
2000年 | 110篇 |
1999年 | 125篇 |
1998年 | 100篇 |
1997年 | 68篇 |
1996年 | 68篇 |
1995年 | 68篇 |
1994年 | 65篇 |
1993年 | 59篇 |
1992年 | 101篇 |
1991年 | 89篇 |
1990年 | 65篇 |
1989年 | 72篇 |
1988年 | 64篇 |
1987年 | 71篇 |
1986年 | 57篇 |
1985年 | 70篇 |
1984年 | 63篇 |
1983年 | 52篇 |
1982年 | 41篇 |
1981年 | 47篇 |
1980年 | 43篇 |
1979年 | 64篇 |
1978年 | 55篇 |
1977年 | 45篇 |
1976年 | 43篇 |
1975年 | 40篇 |
1974年 | 33篇 |
排序方式: 共有10000条查询结果,搜索用时 265 毫秒
11.
12.
Alicia P. Higueruelo Adrian Schreyer G. Richard J Bickerton Tom L. Blundell Will R. Pitt 《PloS one》2012,7(12)
Efforts to increase affinity in the design of new therapeutic molecules have tended to lead to greater lipophilicity, a factor that is generally agreed to be contributing to the low success rate of new drug candidates. Our aim is to provide a structural perspective to the study of lipophilic efficiency and to compare molecular interactions created over evolutionary time with those designed by humans. We show that natural complexes typically engage in more polar contacts than synthetic molecules bound to proteins. The synthetic molecules also have a higher proportion of unmatched heteroatoms at the interface than the natural sets. These observations suggest that there are lessons to be learnt from Nature, which could help us to improve the characteristics of man-made molecules. In particular, it is possible to increase the density of polar contacts without increasing lipophilicity and this is best achieved early in discovery while molecules remain relatively small. 相似文献
13.
Douglas G. Gilmour Walter M. Collins Tom L. Fredericksen Willard E. Urban Jr. Paul F. Ward Nancy L. DiFronzo 《Immunogenetics》1986,23(1):1-6
Chickens of Regional Poultry Research Laboratory (RPRL) inbred line 63 regress sarcomas induced by Bryan high-titer Rous sarcoma virus to a greater extent than chickens of line RPRL 100, although these lines are identical for the major histocompatibility B complex. They differ, however, at three independent autosomal loci: Ly-4 and Th-1 determine the surface alloantigens of partly overlapping subsets of T lymphocytes, and Bu-1 determines a surface alloantigen of B lymphocytes. The association of genotypes at these loci with quantitative variation in their ability to regress Rous sarcomas was tested in segregating F4 generation progeny derived from crosses of lines 100 and 63. The Ly-4 and Bu-1 genotypes showed association with Rous sarcoma regression, but the Th-1 genotype did not. Chickens of the Ly-4
a/Ly-4
a, Bu-1
b/Bu-1
b and Ly-4
b/Ly-4
b, Bu-1
a/Bu-1
a genotypes had a significantly higher regressor ability than the other two double homozygous genotypes. These results indicate that higher regression is associated with (1) interaction between the Ly-4 and Bu-1 loci, and (2) complementation between either the line 6 Ly-4
a allele and the line 100 Bu-1
b allele, or the line 100 Ly-4
b allele and the line 6 Bu-1
a allele. 相似文献
14.
Brain and body weights, contents of DNA and protein and activities of 1,6-diphosphofructoaldolase (aldolase, EC 4.1.2.13), creatine phosphokinase (CPK, EC 2.7.3.2), and isocitric dehydrogenase (ICD, EC 1.1.1.42) in brain (minus cerebellum and brain stem) were studied in control and food-deprived rats at 7, 14 and 21 days of postnatal age. Activities of all three enzymes per brain were less in the food-deprived animals. In both groups of rats the ratios of aldolase/DNA and CPK/DNA increased with maturation, indicating that increasing activity per brain during maturation was the result of both increased activity per cell and increased numbers of cells. The ratio of ICD/DNA decreased with maturation but was essentially the same in both the food-deprived and control groups. Increase of ICD activity per brain with maturation was attributable to increased numbers of cells. Food deprivation in immature animals resulted in lowered activities per brain for aldolase, CPK and ICD because of diminished cell multiplication. 相似文献
15.
16.
Nina Fudge Charles D A Wolfe Christopher McKevitt 《BMJ (Clinical research ed.)》2008,336(7639):313-317
Objectives To understand how the policy of user involvement is interpreted in health service organisations and to identify factors that influence how user involvement is put into practice.Design Ethnographic study using participant observation, interviews, and collection of documentary evidence.Setting A multiagency modernisation programme to improve stroke services in two London boroughs.Participants Service users, National Health Service managers, and clinicians.Results User involvement in the programme was initiated and led by professionals. Professionals determined the areas of service improvement service users could participate in. A wide range of activities were considered “user involvement,” from patient satisfaction surveys to service users delivering peer support. Involvement tended to be most active in the least technical areas and areas with least input from clinicians. Factors that might explain this included organisational structure, the vagueness of the concept of user involvement, the value attributed to service users’ experiential knowledge, and variations in professional and service user understandings of and commitment to involvement. The gains of involvement were harder to identify in terms of impact on services. More evident were the personal gains for those involved: satisfaction of feeling listened to by professionals, social opportunities of meeting others in a similar situation, and increased knowledge about stroke and services available.Conclusions User involvement may not automatically lead to improved service quality. Healthcare professionals and service users understand and practise user involvement in different ways according to individual ideologies, circumstances, and needs. Given the resource implications of undertaking user involvement in service development there is a need for critical debate on the purpose of such involvement as well as better evidence of the benefits claimed for it. 相似文献
17.
Summary Cellular fatty acids inLactobacillus büchneri were transmethylated with H2SO4 catalyst in methanol at elevated temperature. By optimising the reaction time and the amounts of catalyst and methanol used at a fixed temperature it was possible to maximise the lactobacillic acid yield. The yield of lactobacillic acid with this method was better than with the traditional method using base-catalysed saponification followed by HCL-catalysed methylation. 相似文献
18.
Tom M. Spight 《Journal of experimental marine biology and ecology》1973,13(3):215-228
Shell shape, as expressed in terms of log weight/log length, (relative weight), changes progressively as Thais lamellosa Gmelin grows. Relative weight decreases at all sites, but the decrease is relatively rapid at localities with low food supply and correspondingly low growth rates. Snails at these sites are ‘slender’ relative to those at sites with greater barnacle populations. The sitespecific shapes are phenotypic, since snails with fat and with slender parents all became slender in the laboratory where food was readily available and growth rates were higher than those observed at most field localities. Since these snails did not become ‘fat’, food abundance is not the immediately causal, environmental factor promoting a slow change in relative weight. Two other species of Thais live in the same places and undergo similar changes in relative weight. 相似文献
19.
A cohort of 169 births to women who were exposed throughout pregnancy to chloroquine 300 mg base once a week for chemosuppression of malaria was studied. The birth defects in this cohort were compared with those in a control group of 454 births to women who were not exposed to chloroquine, most of whom lived in non-malarious areas. The proportion of birth defects in the exposed group was not significantly different from that in the control group. This observation must be considered within the limitations of the study, which could detect only a strong teratogenic effect. It could not exclude risks lower than a 5.7-fold increase in the incidence of birth defects when chloroquine was used. Women using chloroquine during pregnancy for chemosuppression of malaria can be reassured that it is not a strong teratogen, but if it is to be used the risk of developing malaria should be balanced against the lack of data to determine whether it carries a low teratogenic risk. 相似文献
20.