全文获取类型
收费全文 | 574篇 |
免费 | 37篇 |
国内免费 | 4篇 |
出版年
2022年 | 4篇 |
2021年 | 9篇 |
2020年 | 5篇 |
2019年 | 7篇 |
2018年 | 11篇 |
2017年 | 14篇 |
2016年 | 18篇 |
2015年 | 24篇 |
2014年 | 22篇 |
2013年 | 28篇 |
2012年 | 29篇 |
2011年 | 23篇 |
2010年 | 14篇 |
2009年 | 17篇 |
2008年 | 21篇 |
2007年 | 20篇 |
2006年 | 25篇 |
2005年 | 17篇 |
2004年 | 26篇 |
2003年 | 16篇 |
2002年 | 20篇 |
2001年 | 26篇 |
2000年 | 16篇 |
1999年 | 13篇 |
1998年 | 12篇 |
1997年 | 4篇 |
1996年 | 8篇 |
1995年 | 6篇 |
1993年 | 4篇 |
1992年 | 6篇 |
1991年 | 7篇 |
1988年 | 12篇 |
1987年 | 11篇 |
1986年 | 4篇 |
1985年 | 6篇 |
1984年 | 6篇 |
1983年 | 4篇 |
1980年 | 4篇 |
1979年 | 8篇 |
1978年 | 4篇 |
1973年 | 7篇 |
1972年 | 4篇 |
1971年 | 7篇 |
1970年 | 8篇 |
1969年 | 7篇 |
1967年 | 8篇 |
1966年 | 7篇 |
1889年 | 3篇 |
1887年 | 4篇 |
1886年 | 3篇 |
排序方式: 共有615条查询结果,搜索用时 15 毫秒
71.
B Kot A Oszajca A Jakubczak K Bukowski A Wo?niak-Kosek 《Medycyna do?wiadczalna i mikrobiologia》2001,53(1):31-38
The aim of the study was to evaluate the hydrophobic properties of Yersinia enterocolitica and to determine the influence of the culture conditions, such as: type of medium, temperature, and duration of the culture on the manifestation of these properties. The subject of the study were 117 of Y. enterocolitica strains isolated from humans and pigs. The ammonium sulphate salt aggregation test according to Lindahl modified method was used to evaluate the hydrophobic properties of Y. enterocolitica strains. Strains of Y. enterocolitica were cultured for 24 h at 25 degrees C on TSA (Difco) medium. During investigation of the influence of the culture conditions the chosen strains were incubated for 24 h and 48 h at 25 degrees C and 37 degrees C on TSA (Difco), LB (Difco), enrichment agar (Biomed), and enrichment agar with 5% sheep blood (Graso). A total of 44.5%, 17.9%, 9.4%, and 28.2% strains of Y. enterocolitica showed very strong hydrophobic properties, strong hydrophobic properties, some hydrophobic properties, and were non-hydrophobic, respectively when strains of Y. enterocolitica were cultured for 24 h at 25 degrees C on TSA medium. A total of 75.5% strains isolated from humans showed very strong hydrophobic properties and 13.5% strains were non-hydrophobic. Among strains isolated from pigs 30% showed very strong hydrophobic properties but 35% were non-hydrophobic. The hydrophobic properties of Y. enterocolitica depended on the temperature, duration of the culture and the type of media. The highest number of strains with very strong hydrophobic properties (89.6%) was obtained after 48 h of the incubation at 37 degrees C on the enrichment agar with 5% sheep blood. The highest number of non-hydrophobic strains of Y. enterocolitica (28.5%) was obtained after 24 h at 25 degrees C on TSA medium. 相似文献
72.
Insulin-like growth factor I-dependent regulation of prolidase activity in cultured human skin fibroblasts 总被引:3,自引:0,他引:3
Miltyk Wojciech Karna Ewa Wołczyński Sławomir Pałka Jerzy 《Molecular and cellular biochemistry》1998,186(1-2):177-184
The objectives of this article are to: (i) discuss the origins and the nature of ischemic injury, (ii) identify factors influencing the evolution of injury, (iii) consider various cellular targets for ischemic injury, (iv) assess the overall importance of reperfusion injury, (v) discuss conceptual approaches to cardioprotection and (vi) to identify new ideas and approaches in the realm of myocardial protection. In the human heart, myocardial ischemia may take many forms, it may exist for periods as short as a few seconds or minutes, it may last for hours or it may persist for years. In terms of discussing interventions to combat myocardial ischemia, this article will focus on: (i) regional ischemia as occurs during evolving myocardial infarction and (ii) whole heart or global ischemia as occurs during cardiac surgery and transplantation. 相似文献
73.
74.
75.
Kaja Wierucka Sonia Siemianowska Marta Woźniak Katarzyna Jasnosz Magdalena Kieliszczyk Paulina Kozak 《Journal of applied animal welfare science : JAAWS》2016,19(1):62-72
Ethograms and time budgets are crucial for the behavioral assessment of nonhuman animals in zoos, and they serve as references for welfare research. This study was conducted to obtain detailed time budgets of trained Cape fur seals (Arctocephalus pusillus) in captivity, to evaluate variations of these patterns, and to determine whether abnormal behaviors had been displayed. Behavioral data for 3 Cape fur seals in the Wroclaw Zoo were collected, and more than 300 observation hours (during a 12-month period) per individual were analyzed. The studied animals exhibited a diversified repertoire of natural behaviors with apparent seasonal and daily patterns, and they did not present stereotypic behaviors. Significant differences of interaction rates between individuals suggest more frequent affiliative interactions among related animals. The absence of stereotypic behaviors, good health of individuals, and the presence of diversified natural behaviors indicated relatively good welfare of Cape fur seals kept in the Wroclaw Zoo. 相似文献
76.
77.
Microstructure and nanomechanical properties of single stalks from diatom Didymosphenia geminata and their change due to adsorption of selected metal ions
下载免费PDF全文
![点击此处可从《Journal of phycology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Izabela Zgłobicka Adrian Chlanda Michał Woźniak Maciej Łojkowski Robert Szoszkiewicz Marta Mazurkiewicz‐Pawlicka Wojciech Święszkowski Elżbieta Wyroba Krzysztof J. Kurzydłowski 《Journal of phycology》2017,53(4):880-888
We present topographical and nanomechanical characterization of single Didymosphenia geminata stalk. We compared the samples before and after adsorption of metal ions from freshwater samples. Transmission electron microscopy studies of single stalk cross‐sections have shown three distinct layers and an additional thin extra coat on the external layer (called “EL”). Using scanning electron microscopy and atomic force microscopy (AFM), we found that topography of single stalks after ionic adsorption differed significantly from topography of pristine stalks. AFM nanoindentation studies in ambient conditions yielded elastic moduli of 214 ± 170 MPa for pristine stalks and 294 ± 108 MPa for stalks after ionic adsorption. Statistical tests showed that those results were significantly different. We conducted only preliminary comparisons between ionic adsorption of several stalks in air and in water. While the stalks with ions were on average stiffer than the pristine stalks in air, they became more compliant than the pristine stalks in water. We also heated the stalks and detected EL softening at 50°C ± 15°C. AFM nanoindentation in air on the softened samples yielded elastic moduli of 26 ± 9 MPa for pristine samples and 43 ± 22 MPa for stalks with absorbed metal ions. Substantial decrease of the EL elastic moduli after heating was expected. Significantly different elastic moduli for the samples after ionic adsorption in both cases (i.e., for heated and nonheated samples), as well as behavior of the stalks immersed in water, point to permanent structural EL changes due to ions. 相似文献
78.
Sophie S.W. Wang Ching‐Min Huang Yi‐Wei Lee Michael Isaac Chen Szu‐An Chuang Shu‐Hua Chen Ying‐Wei Lu Chun‐Cheng Lin Ka‐Wo Lee Wen‐Hung Hsu Kun‐Pin Wu Yu‐Ju Chen 《Proteomics》2017,17(11)
We reported an integrated platform to explore serum protein variant pattern in cancer and its utility as a new class of biomarker panel for diagnosis. On the model study of serum amyloid A (SAA), we employed nanoprobe‐based affinity mass spectrometry for enrichment, identification and quantitation of SAA variants from serum of 105 gastric cancer patients in comparison with 54 gastritis patients, 54 controls, and 120 patients from other cancer. The result revealed surprisingly heterogeneous and most comprehensive SAA bar code to date, which comprises 24 SAA variants including SAA1‐ and SAA2‐encoded products, polymorphic isoforms, N‐terminal–truncated forms, and three novel SAA oxidized isotypes, in which the variant‐specific peptide sequence were also confirmed by LC‐MS/MS. A diagnostic model was developed for dimension reduction and computational classification of the 24 SAA‐variant bar code, providing good discrimination (AUC = 0.85 ± 3.2E?3) for differentiating gastric cancer group from gastritis and normal groups (sensitivity, 0.76; specificity, 0.81) and was validated with external validation cohort (sensitivity, 0.71; specificity, 0.74). Our platform not only shed light on the occurrence and modification extent of under‐represented serum protein variants in cancer, but also suggested a new concept of diagnostic platform by serum protein variant profile. 相似文献
79.
Front Cover: Exploring the expression bar code of SAA variants for gastric cancer detection
下载免费PDF全文
![点击此处可从《Proteomics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
80.
Jifeng Tang Samantha J Baldwin Jeanne ME Jacobs C Gerard van der Linden Roeland E Voorrips Jack AM Leunissen Herman van Eck Ben Vosman 《BMC bioinformatics》2008,9(1):374